Cryo-EM structure of C6-ceramide-bound SPT-ORMDL3 complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7yiu
Q-score: 0.616
Xie T, Liu P, Wu X, Dong F, Zhang Z, Yue J, Mahawar U, Farooq F, Vohra H, Fang Q, Liu W, Wattenberg BW, Gong X
Nat Commun (2023) 14 pp. 3475-3475 [ Pubmed: 37308477 DOI: doi:10.1038/s41467-023-39274-y ]
- N-((e,2s,3r)-1,3-dihydroxyoctadec-4-en-2-yl)hexanamide (397 Da, Ligand)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Serine palmitoyltransferase 1 (46 kDa, Protein from Homo sapiens)
- Hexadecane (226 Da, Ligand)
- Serine palmitoyltransferase small subunit a (10 kDa, Protein from Homo sapiens)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Homo sapiens)
- Orm1-like protein 3 (17 kDa, Protein from Homo sapiens)
- Spt-ormdl3 complex (Complex from Homo sapiens)
- Serine palmitoyltransferase 1 (5 kDa, Protein from Homo sapiens)
Cryo-EM structure of SPT-ORMDL3 complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7yiy
Q-score: 0.637
Xie T, Liu P, Wu X, Dong F, Zhang Z, Yue J, Mahawar U, Farooq F, Vohra H, Fang Q, Liu W, Wattenberg BW, Gong X
Nat Commun (2023) 14 pp. 3475-3475 [ Pubmed: 37308477 DOI: doi:10.1038/s41467-023-39274-y ]
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Serine palmitoyltransferase 1 (46 kDa, Protein from Homo sapiens)
- Serine palmitoyltransferase small subunit a (10 kDa, Protein from Homo sapiens)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Homo sapiens)
- Orm1-like protein 3 (17 kDa, Protein from Homo sapiens)
- Spt-ormdl3 complex (Complex from Homo sapiens)
- Serine palmitoyltransferase 1 (5 kDa, Protein from Homo sapiens)
Cryo-EM structure of SPT-ORMDL3 (ORMDL3-deltaN2) complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7yj1
Q-score: 0.58
Xie T, Liu P, Wu X, Dong F, Zhang Z, Yue J, Mahawar U, Farooq F, Vohra H, Fang Q, Liu W, Wattenberg BW, Gong X
Nat Commun (2023) 14 pp. 3475-3475 [ Pubmed: 37308477 DOI: doi:10.1038/s41467-023-39274-y ]
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Serine palmitoyltransferase 1 (46 kDa, Protein from Homo sapiens)
- Orm1-like protein 3 (17 kDa, Protein from Homo sapiens)
- Serine palmitoyltransferase small subunit a (10 kDa, Protein from Homo sapiens)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Homo sapiens)
- Spt-ormdl3 complex (Complex from Homo sapiens)
- Serine palmitoyltransferase 1 (5 kDa, Protein from Homo sapiens)
Cryo-EM structure of SPT-ORMDL3 (ORMDL3-N13A) complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7yj2
Q-score: 0.611
Xie T, Liu P, Wu X, Dong F, Zhang Z, Yue J, Mahawar U, Farooq F, Vohra H, Fang Q, Liu W, Wattenberg BW, Gong X
Nat Commun (2023) 14 pp. 3475-3475 [ Pubmed: 37308477 DOI: doi:10.1038/s41467-023-39274-y ]
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Serine palmitoyltransferase 1 (46 kDa, Protein from Homo sapiens)
- Orm1-like protein 3 (17 kDa, Protein from Homo sapiens)
- Serine palmitoyltransferase small subunit a (10 kDa, Protein from Homo sapiens)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Homo sapiens)
- Spt-ormdl3 complex (Complex from Homo sapiens)
- Serine palmitoyltransferase 1 (5 kDa, Protein from Homo sapiens)
Cryo-EM structure of the dimeric atSPT-ORM1 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yjk
Q-score: 0.556
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ Pubmed: 36989369 DOI: doi:10.1126/sciadv.adg0728 ]
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Long chain base biosynthesis protein 1 (53 kDa, Protein from Arabidopsis thaliana)
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 2a (54 kDa, Protein from Arabidopsis thaliana)
Cryo-EM structure of the monomeric atSPT-ORM1 complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7yjm
Q-score: 0.585
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ Pubmed: 36989369 DOI: doi:10.1126/sciadv.adg0728 ]
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Atlcb1 (46 kDa, Protein from Arabidopsis thaliana)
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Atlcb1 (6 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 2a (54 kDa, Protein from Arabidopsis thaliana)
Cryo-EM structure of the monomeric atSPT-ORM1 (ORM1-N17A) complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7yjn
Q-score: 0.526
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ Pubmed: 36989369 DOI: doi:10.1126/sciadv.adg0728 ]
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 1 (6 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 1 (46 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 2a (54 kDa, Protein from Arabidopsis thaliana)
Cryo-EM structure of the monomeric atSPT-ORM1 (LCB2a-deltaN5) complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7yjo
Q-score: 0.621
Liu P, Xie T, Wu X, Han G, Gupta SD, Zhang Z, Yue J, Dong F, Gable K, Niranjanakumari S, Li W, Wang L, Liu W, Yao R, Cahoon EB, Dunn TM, Gong X
Sci Adv (2023) 9 pp. eadg0728-eadg0728 [ Pubmed: 36989369 DOI: doi:10.1126/sciadv.adg0728 ]
- Spt-orm1 complex (Complex from Arabidopsis thaliana)
- Transmembrane protein, putative (duf3317) (9 kDa, Protein from Arabidopsis thaliana)
- N-[(2s,3r,4e)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide (650 Da, Ligand)
- Atlcb1 (46 kDa, Protein from Arabidopsis thaliana)
- Long chain base biosynthesis protein 2a (53 kDa, Protein from Arabidopsis thaliana)
- [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine (336 Da, Ligand)
- Ormdl family protein (18 kDa, Protein from Arabidopsis thaliana)
- Atlcb1 (6 kDa, Protein from Arabidopsis thaliana)