Structure of an orphan GPCR-G protein signaling complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7wz4
Q-score: 0.523
Chen G, Xu J, Inoue A, Schmidt MF, Bai C, Lu Q, Gmeiner P, Liu Z, Du Y
Nat Commun (2022) 13 pp. 2375-2375 [ DOI: doi:10.1038/s41467-022-30081-5 Pubmed: 35501348 ]
- Gpr88-gi1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Gpr88-gi1 signaling complex (Complex)
- Probable g-protein coupled receptor 88 (55 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7w6p
Q-score: 0.056
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ Pubmed: 36173843 DOI: doi:10.1126/science.abn7065 ]
- N-pyridin-4-ylisoquinolin-4-amine (221 Da, Ligand)
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
Cryo-EM structure of the alpha2A adrenergic receptor GoA signaling complex bound to a biased agonist
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7w7e
Q-score: 0.466
Fink EA, Xu J, Hubner H, Braz JM, Seemann P, Avet C, Craik V, Weikert D, Schmidt MF, Webb CM, Tolmachova NA, Moroz YS, Huang XP, Kalyanaraman C, Gahbauer S, Chen G, Liu Z, Jacobson MP, Irwin JJ, Bouvier M, Du Y, Shoichet BK, Basbaum AI, Gmeiner P
Science (2022) 377 pp. eabn7065-eabn7065 [ Pubmed: 36173843 DOI: doi:10.1126/science.abn7065 ]
- 5-(3-bicyclo[4.2.0]octa-1,3,5-trienyl)-1,2,3,6-tetrahydropyridine (185 Da, Ligand)
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Signaling complex of alpha2a adrenergic receptor with goa (Complex from Homo sapiens)
Structure of the alpha2A-adrenergic receptor GoA signaling complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ej0
Q-score: 0.426
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Scfv16 (32 kDa, Protein from Mus musculus)
- Noradrenaline (170 Da, Ligand)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to brimonidine
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ej8
Q-score: 0.482
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Scfv16 (32 kDa, Protein from Mus musculus)
- Brimonidine (292 Da, Ligand)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to dexmedetomidine
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7eja
Q-score: 0.378
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Scfv16 (32 kDa, Protein from Mus musculus)
- 4-[(1~{s})-1-(2,3-dimethylphenyl)ethyl]-1~{h}-imidazole (200 Da, Ligand)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
Structure of the alpha2A-adrenergic receptor GoA signaling complex bound to oxymetazoline
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ejk
Q-score: 0.453
Xu J, Cao S, Hubner H, Weikert D, Chen G, Lu Q, Yuan D, Gmeiner P, Liu Z, Du Y
Sci Adv (2022) 8 pp. eabj5347-eabj5347 [ DOI: doi:10.1126/sciadv.abj5347 Pubmed: 35245122 ]
- Scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Alpha2a-adrenergic receptor go signaling complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor (50 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Oxymetazoline (260 Da, Ligand)
- Alpha2a-adrenergic receptor go signaling complex (Complex from Homo sapiens)
Structure of the GPR88-Gi1 signaling complex bound to a synthetic ligand
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7ejx
Q-score: 0.606
Chen G, Xu J, Inoue A, Schmidt MF, Bai C, Lu Q, Gmeiner P, Liu Z, Du Y
Nat Commun (2022) 13 pp. 2375-2375 [ DOI: doi:10.1038/s41467-022-30081-5 Pubmed: 35501348 ]
- Gpr88-gi1 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Gpr88-gi1 signaling complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (1r,2r)-n-[(2s,3s)-2-azanyl-3-methyl-pentyl]-n-[4-(4-propylphenyl)phenyl]-2-pyridin-2-yl-cyclopropane-1-carboxamide (455 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Probable g-protein coupled receptor 88 (40 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
cryo-EM structure of alpha2BAR-GoA complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6k41
Q-score: 0.556
Yuan D, Liu Z, Kaindl J, Maeda S, Zhao J, Sun X, Xu J, Gmeiner P, Wang HW, Kobilka BK
Nat Chem Biol (2020) 16 pp. 507-512 [ Pubmed: 32152538 DOI: doi:10.1038/s41589-020-0492-2 ]
- 4-[(1~{s})-1-(2,3-dimethylphenyl)ethyl]-1~{h}-imidazole (200 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Mus musculus)
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor,endolysin,alpha-2b adrenergic receptor,alpha-2b adrenergic receptor (58 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha2bar-goa complex (150 kDa, Complex from Spodoptera)
cryo-EM structure of alpha2BAR-Gi1 complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6k42
Q-score: 0.299
Yuan D, Liu Z, Kaindl J, Maeda S, Zhao J, Sun X, Xu J, Gmeiner P, Wang HW, Kobilka BK
Nat Chem Biol (2020) 16 pp. 507-512 [ Pubmed: 32152538 DOI: doi:10.1038/s41589-020-0492-2 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Bos taurus)
- 4-[(1~{s})-1-(2,3-dimethylphenyl)ethyl]-1~{h}-imidazole (200 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Mus musculus)
- Scfv (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Alpha-2a adrenergic receptor,endolysin,alpha-2b adrenergic receptor,alpha-2b adrenergic receptor (58 kDa, Protein from Homo sapiens)
- Alpha2bar-gi1 complex (150 kDa, Complex from Spodoptera)