CryoEM structure of ZIKV rsNS1
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8wn8
Q-score: 0.33
Chew BLA, Ngoh AQ, Phoo WW, Weng MJG, Sheng HJ, Chan KWK, Tan EYJ, Gelbart T, Xu C, Tan GS, Vasudevan SG, Luo D
Npj Viruses (2024) 2 pp. 14- [ DOI: doi:10.1038/s44298-024-00024-6 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Zika virus (Virus from Zika virus)
- Non-structural protein 1 (41 kDa, Protein from Zika virus)
ZIKV rsNS1 in complex with Fab AA12 and anti-fab nanobody
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8wnp
Q-score: 0.428
Chew BLA, Ngoh AQ, Phoo WW, Weng MJG, Sheng HJ, Chan KWK, Tan EYJ, Gelbart T, Xu C, Tan GS, Vasudevan SG, Luo D
Npj Viruses (2024) 2 pp. 14- [ DOI: doi:10.1038/s44298-024-00024-6 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Non-structural protein 1 (40 kDa, Protein from Zika virus)
- Zikv rsns1 in complex with fab aa12 and anti-fab nanobody (Complex from Zika virus)
- Vh chains (23 kDa, Protein from Homo sapiens)
- Vl chains (23 kDa, Protein from Homo sapiens)
ZIKV rsNS1 in complex with Fab GB5 and anti-fab nanobody
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8wnu
Q-score: 0.331
Chew BLA, Ngoh AQ, Phoo WW, Weng MJG, Sheng HJ, Chan KWK, Tan EYJ, Gelbart T, Xu C, Tan GS, Vasudevan SG, Luo D
Npj Viruses (2024) 2 pp. 14- [ DOI: doi:10.1038/s44298-024-00024-6 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Non-structural protein 1 (40 kDa, Protein from Zika virus)
- Vh (23 kDa, Protein from Homo sapiens)
- Zikv rsns1 in complex with fab gb5 and anti-fab nanobody (Complex from Zika virus)
- Vl (23 kDa, Protein from Homo sapiens)
CryoEM structure of ZIKV rsNS1 filament
Resolution: 8.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 8wo0
Q-score: 0.088
Chew BLA, Ngoh AQ, Phoo WW, Weng MJG, Sheng HJ, Chan KWK, Tan EYJ, Gelbart T, Xu C, Tan GS, Vasudevan SG, Luo D
Npj Viruses (2024) 2 pp. 14- [ DOI: doi:10.1038/s44298-024-00024-6 ]
- Zikv recombinantly secreted ns1 filament (Complex from Zika virus)
- Non-structural protein 1 (40 kDa, Protein from Zika virus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
ZIKV rsNS1 in complex with Fab EB9 and anti-fab nanobody
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8wo4
Q-score: 0.416
Chew BLA, Ngoh AQ, Phoo WW, Weng MJG, Sheng HJ, Chan KWK, Tan EYJ, Gelbart T, Xu C, Tan GS, Vasudevan SG, Luo D
Npj Viruses (2024) 2 pp. 14- [ DOI: doi:10.1038/s44298-024-00024-6 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Vl (23 kDa, Protein from Homo sapiens)
- Vh (24 kDa, Protein from Homo sapiens)
- Zikv rsns1 in complex with fab aa12 and anti-fab nanobody (Complex from Homo sapiens)
- Anti-fab nanobody (15 kDa, Protein from Lama glama)
- Non-structural protein 1 (41 kDa, Protein from Zika virus)
cryo-EM structure of human TMEM63A
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8wua
Q-score: 0.241
Wu X, Shang T, Lu X, Luo D, Yang D
Proteins (2024) 92 pp. 750-756 [ Pubmed: 38217391 DOI: doi:10.1002/prot.26660 ]
- Csc1-like protein 1 (82 kDa, Protein from Homo sapiens)
- Human tmem63a (Complex from Homo sapiens)
CryoEM structure of isNS1 in complex with Fab56.2 and HDL
Chew BLA, Ngoh ANQ, Phoo WW, Chan KWK, Ser Z, Tulsian NK, Lim SS, Weng MJG, Watanabe S, Choy MM, Low J, Ooi EE, Ruedl C, Sobota RM, Vasudevan SG, Luo D
eLife (2024) 12 [ DOI: doi:10.7554/eLife.90762 Pubmed: 38787378 ]
- Ternary complex of fab56.2 with dengue virus nonstructural protein 1 and high density lipoprotein (Complex)
- Fab 56.2 heavy chain (Macromolecule from Mus musculus)
- Core protein (Macromolecule from Dengue virus type 2)
- Isns1ts (Complex from Dengue virus type 2)
- Monoclonal antibody fragment 56.2 (Complex from Mus musculus)
- Fab 56.2 light chain (Macromolecule from Mus musculus)
CryoEM structure of isNS1 in complex with Fab56.2
Chew BLA, Ngoh ANQ, Phoo WW, Chan KWK, Ser Z, Tulsian NK, Lim SS, Weng MJG, Watanabe S, Choy MM, Low J, Ooi EE, Ruedl C, Sobota RM, Vasudevan SG, Luo D
eLife (2024) 12 [ DOI: doi:10.7554/eLife.90762 Pubmed: 38787378 ]
- Ternary complex of fab56.2 with dengue virus nonstructural protein 1 and high density lipoprotein (Complex)
- Fab56.2 heavy chain (Macromolecule from Mus musculus)
- Fab56.2 (Complex from Mus musculus)
- Fab56.2 light chain (Macromolecule from Mus musculus)
- Core protein (Macromolecule from dengue virus type 2)
- Isns1ts (Complex from Dengue virus type 2)
Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GpppAmU
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 8jce
Q-score: 0.643
Law MCY, Zhang K, Tan YB, Nguyen TM, Luo D
J Biol Chem (2023) 299 pp. 105415-105415 [ DOI: doi:10.1016/j.jbc.2023.105415 Pubmed: 37918803 ]
- Zinc ion (65 Da, Ligand)
- Mrna-capping enzyme nsp1 (61 kDa, Protein from Chikungunya virus strain S27-African prototype)
- [(2~{r},3~{r},4~{r},5~{r})-5-(6-azanyl-7,8-dihydropurin-9-yl)-2-[[[[[(2~{r},3~{s},4~{r},5~{r})-5-(2-azanyl-7-methyl-6-oxidanylidene-1,8-dihydropurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-4-methoxy-oxolan-3-yl] [(2~{r},3~{s},4~{r},5~{s})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate (1 kDa, Ligand)
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
In situ structure of the non-replicative Chikungunya virus nonstructural protein complex
Tan YB, Chmielewski D, Law MCY, Zhang K, He Y, Chen M, Jin J, Luo D
Sci Adv (2022) 8 pp. eadd2536-eadd2536 [ DOI: doi:10.1126/sciadv.add2536 Pubmed: 36449616 ]