In situ structure of the non-replicative Chikungunya virus nonstructural protein complex
Tan YB, Chmielewski D, Law MCY, Zhang K, He Y, Chen M, Jin J, Luo D
Sci Adv (2022) 8 pp. eadd2536-eadd2536 [ Pubmed: 36449616 DOI: doi:10.1126/sciadv.add2536 ]
Molecular architecture of the chikungunya virus replication complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7y38
Q-score: 0.539
Tan YB, Chmielewski D, Law MCY, Zhang K, He Y, Chen M, Jin J, Luo D
Sci Adv (2022) 8 pp. eadd2536-eadd2536 [ Pubmed: 36449616 DOI: doi:10.1126/sciadv.add2536 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Alphavirus replication complex (100 kDa, Complex)
- Rna-directed rna polymerase nsp4 (68 kDa, Protein from Onyong-nyong virus)
- Protease nsp2 (89 kDa, Protein from Chikungunya virus strain S27-African prototype)
- Mrna-capping enzyme nsp1,affinity-tag (strepii-3xflag) (64 kDa, Protein from Chikungunya virus strain S27-African prototype)
- Zinc ion (65 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nsp1 dodecameric ring (Complex from Chikungunya virus strain S27-African prototype)
- Nsp2 helicase-protease; docked on top of viral complex with synthetic rna bound (Complex from Chikungunya virus strain S27-African prototype)
- Nsp4 rdrp; docked within nsp1 central cavity (Complex from Onyong-nyong virus)
- Rna (5'-r(p*cp*cp*a)-3') (894 Da, RNA from in vitro transcription vector pT7-TP(deltai))
In situ structure of the Chikungunya virus replication complex
Tan YB, Chmielewski D, Law MCY, Zhang K, He Y, Chen M, Jin J, Luo D
Sci Adv (2022) 8 pp. eadd2536-eadd2536 [ Pubmed: 36449616 DOI: doi:10.1126/sciadv.add2536 ]
MDA5 CARDs-MAVS CARD polyUb complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7dni
Q-score: 0.497
Song B, Chen Y, Liu X, Yuan F, Tan EYJ, Lei Y, Song N, Han Y, Pascal BD, Griffin PR, Luo C, Wu B, Luo D, Zheng J
Immunity (2021) 54 pp. 2218- [ DOI: doi:10.1016/j.immuni.2021.09.008 Pubmed: 34644557 ]
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- K63-polyub bound mda5cards-mavscard complex (Complex from Homo sapiens)
- Mitochondrial antiviral-signaling protein (11 kDa, Protein from Homo sapiens)
- Interferon-induced helicase c domain-containing protein 1 (23 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7dnj
Q-score: 0.48
Song B, Chen Y, Liu X, Yuan F, Tan EYJ, Lei Y, Song N, Han Y, Pascal BD, Griffin PR, Luo C, Wu B, Luo D, Zheng J
Immunity (2021) 54 pp. 2218- [ DOI: doi:10.1016/j.immuni.2021.09.008 Pubmed: 34644557 ]
- Interferon-induced helicase c domain-containing protein 1 (23 kDa, Protein from Homo sapiens)
- K63polyub (Complex from Homo sapiens)
- Ubiquitin (8 kDa, Protein from Homo sapiens)
- Mda5 cards (Complex)
- K63polyub bound mda5 cards complex (Complex from Homo sapiens)