Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
Structure of rat beta-arrestin 1 bound to allosteric inhibitor
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9dnm
Q-score: 0.466
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14r,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one (366 Da, Ligand)
Stabilized beta-arrestin 1-V2T subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6ni2
Q-score: 0.348
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ Pubmed: 31740855 DOI: doi:10.1038/s41594-019-0330-y ]
- Stabilized beta-arrestin 1-v2t subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Vasopressin 2 tail (Complex from Homo sapiens)
- Beta-arrestin-1 (Complex from Bos taurus)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Fab30 light chain (23 kDa, Protein from synthetic construct)
- Nanobody 32 (Complex from Lama glama)
- Fab30 heavy chain (25 kDa, Protein from synthetic construct)
- Beta-arrestin-1 (44 kDa, Protein from Bos taurus)
- Fab30 (Complex from synthetic construct)
B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ni3
Q-score: 0.39
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ Pubmed: 31740855 DOI: doi:10.1038/s41594-019-0330-y ]
- Endolysin,beta-2 adrenergic receptor chimera (57 kDa, Protein from Homo sapiens)
- B2v2r-gs protein subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- 8-[(1r)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2h-1,4-benzoxazin-3(4h)-one (370 Da, Ligand)
- B2v2r-gs protein subcomplex (Complex from Homo sapiens)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
Structure of a GPCR-G protein-beta-arrestin mega-complex
Nguyen AH, Thomsen ARB, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ Pubmed: 31740855 DOI: doi:10.1038/s41594-019-0330-y ]