Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
Structure of rat beta-arrestin 1 bound to allosteric inhibitor
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9dnm
Q-score: 0.466
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14r,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one (366 Da, Ligand)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
Stabilized beta-arrestin 1-V2T subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6ni2
Q-score: 0.348
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- Beta-arrestin-1 (44 kDa, Protein from Bos taurus)
- Fab30 light chain (23 kDa, Protein from synthetic construct)
- Fab30 heavy chain (25 kDa, Protein from synthetic construct)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Vasopressin 2 tail (Complex from Homo sapiens)
- Nanobody 32 (Complex from Lama glama)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Fab30 (Complex from synthetic construct)
- Beta-arrestin-1 (Complex from Bos taurus)
- Stabilized beta-arrestin 1-v2t subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ni3
Q-score: 0.39
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- B2v2r-gs protein subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- B2v2r-gs protein subcomplex (Complex from Homo sapiens)
- Endolysin,beta-2 adrenergic receptor chimera (57 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- 8-[(1r)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2h-1,4-benzoxazin-3(4h)-one (370 Da, Ligand)
Structure of a GPCR-G protein-beta-arrestin mega-complex
Nguyen AH, Thomsen ARB, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]