The potassium-selective channelrhodopsin HcKCR1 and HcKCR2 in lipid nanodisc
Tajima S, Kim YS, Fukuda M, Jo Y, Wang PY, Paggi JM, Inoue M, Byrne EFX, Kishi KE, Nakamura S, Ramakrishnan C, Takaramoto S, Nagata T, Konno M, Sugiura M, Katayama K, Matsui TE, Yamashita K, Kim S, Ikeda H, Kim J, Kandori H, Dror RO, Inoue K, Deisseroth K, Kato HE
Cell (2023) [ Pubmed: 37652010 DOI: 10.1016/j.cell.2023.08.009 ]
Image sets:
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 8h86
Q-score: 0.661
Tajima S, Kim YS, Fukuda M, Jo Y, Wang PY, Paggi JM, Inoue M, Byrne EFX, Kishi KE, Nakamura S, Ramakrishnan C, Takaramoto S, Nagata T, Konno M, Sugiura M, Katayama K, Matsui TE, Yamashita K, Kim S, Ikeda H, Kim J, Kandori H, Dror RO, Inoue K, Deisseroth K, Kato HE
Cell (2023) 186 pp. 4325-4344.e26 [ DOI: doi:10.1016/j.cell.2023.08.009 Pubmed: 37652010 ]
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Retinal (284 Da, Ligand)
- Hckcr1 (Complex from Hyphochytrium catenoides)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Hckcr1 (31 kDa, Protein from Hyphochytrium catenoides)
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8h87
Q-score: 0.682
Tajima S, Kim YS, Fukuda M, Jo Y, Wang PY, Paggi JM, Inoue M, Byrne EFX, Kishi KE, Nakamura S, Ramakrishnan C, Takaramoto S, Nagata T, Konno M, Sugiura M, Katayama K, Matsui TE, Yamashita K, Kim S, Ikeda H, Kim J, Kandori H, Dror RO, Inoue K, Deisseroth K, Kato HE
Cell (2023) 186 pp. 4325-4344.e26 [ DOI: doi:10.1016/j.cell.2023.08.009 Pubmed: 37652010 ]
- Water (18 Da, Ligand)
- Retinal (284 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Hckcr2 (Complex from Hyphochytrium catenoides)
- Hckcr2 (34 kDa, Protein from Hyphochytrium catenoides)
Cryo-EM structure of the potassium-selective channelrhodopsin HcKCR1 H225F mutant in lipid nanodisc
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8iu0
Q-score: 0.636
Tajima S, Kim YS, Fukuda M, Jo Y, Wang PY, Paggi JM, Inoue M, Byrne EFX, Kishi KE, Nakamura S, Ramakrishnan C, Takaramoto S, Nagata T, Konno M, Sugiura M, Katayama K, Matsui TE, Yamashita K, Kim S, Ikeda H, Kim J, Kandori H, Dror RO, Inoue K, Deisseroth K, Kato HE
Cell (2023) 186 pp. 4325-4344.e26 [ DOI: doi:10.1016/j.cell.2023.08.009 Pubmed: 37652010 ]
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Retinal (284 Da, Ligand)
- Hckcr1 (Complex from Hyphochytrium catenoides)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Hckcr1 (31 kDa, Protein from Hyphochytrium catenoides)
The pump-like chanelrhodopsin ChRmine
Kishi KE, Kim YS, Fukuda M, Inoue M, Kusakizako T, Wang PY, Ramakrishnan C, Byrne EFX, Thadhani E, Paggi JM, Matsui TE, Yamashita K, Nagata T, Konno M, Quirin S, Lo M, Benster T, Uemura T, Liu K, Shibata M, Nomura N, Iwata S, Nureki O, Dror RO, Inoue K, Deisseroth K, Kato HE
Cell (2022) [ DOI: 10.1016/j.cell.2022.01.007 Pubmed: 35114111 ]
Image sets:
2.02 angstrom cryo-EM structure of the pump-like channelrhodopsin ChRmine
Resolution: 2.02 Å
EM Method: Single-particle
Fitted PDBs: 7w9w
Q-score: 0.708
Kishi KE, Kim YS, Fukuda M, Inoue M, Kusakizako T, Wang PY, Ramakrishnan C, Byrne EFX, Thadhani E, Paggi JM, Matsui TE, Yamashita K, Nagata T, Konno M, Quirin S, Lo M, Benster T, Uemura T, Liu K, Shibata M, Nomura N, Iwata S, Nureki O, Dror RO, Inoue K, Deisseroth K, Kato HE
Cell (2022) 185 pp. 672- [ Pubmed: 35114111 DOI: doi:10.1016/j.cell.2022.01.007 ]
- Water (18 Da, Ligand)
- Chrmine (Complex from Rhodomonas lens)
- Retinal (284 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Chrmine (35 kDa, Protein from Rhodomonas lens)
2.12 angstrom cryo-EM map of the pump-like channelrhodopsin ChRmine with Fab antibody fragment
Kishi KE, Kim YS, Fukuda M, Inoue M, Kusakizako T, Wang PY, Ramakrishnan C, Byrne EFX, Thadhani E, Paggi JM, Matsui TE, Yamashita K, Nagata T, Konno M, Quirin S, Lo M, Benster T, Uemura T, Liu K, Shibata M, Nomura N, Iwata S, Nureki O, Dror RO, Inoue K, Deisseroth K, Kato HE
Cell (2022) 185 pp. 672-689.e23 [ Pubmed: 35114111 DOI: doi:10.1016/j.cell.2022.01.007 ]
- Chrmine with fab (Protein from Rhodomonas lens)
- Chrmine (Complex from Rhodomonas lens)