CHMP1B/IST1 ssRNA bound copolymer
Resolution: 2.95 Å
EM Method: Helical reconstruction
Fitted PDBs: 8v2q
Q-score: 0.568
Talledge N, Glover J, McCullough J, Alian A, Sadler JBA, Dempsey N, Laughlin TG, Nguyen HC, Wenzel D, Lalonde MS, Ventimiglia LN, LaJoie D, Iwasa J, Starling T, Padilla-Parra S, Ullman KS, Frost A, Sundquist WI, Martin-Serrano J
To Be Published
- Ist1 (Complex from Homo sapiens)
- Charged multivesicular body protein 1b (chmp1b) (Complex from Homo sapiens)
- Ist1 homolog (39 kDa, Protein from Homo sapiens)
- Charged multivesicular body protein 1b (22 kDa, Protein from Homo sapiens)
- Chmp1b/ist1 copolymer bound to a 60-mer oligonucleotide of ssrna (397 kDa, Complex from Homo sapiens)
CryoEM of ssDNA bound CHMP1B/IST1 copolymer assembly
Resolution: 3.01 Å
EM Method: Helical reconstruction
Fitted PDBs: 8v2r
Q-score: 0.551
Talledge N, Glover J, McCullough J, Alian A, Sadler JBA, Dempsey N, Laughlin TG, Nguyen HC, Wenzel D, Lalonde MS, Ventimiglia LN, LaJoie D, Iwasa J, Starling T, Padilla-Parra S, Ullman KS, Frost A, Sundquist WI, Martin-Serrano J
To Be Published
- Chmp1b/ist1 copolymer bound to a 60-mer oligonucleotide of ssdna (397 kDa, Complex from Homo sapiens)
- Ist1 (Complex from Homo sapiens)
- Charged multivesicular body protein 1b (chmp1b) (Complex from Homo sapiens)
- Ist1 homolog (39 kDa, Protein from Homo sapiens)
- Charged multivesicular body protein 1b (22 kDa, Protein from Homo sapiens)
CHMP1B/IST1 dsDNA bound copolymer
Resolution: 2.72 Å
EM Method: Helical reconstruction
Fitted PDBs: 8v2s
Q-score: 0.6
Talledge N, Glover J, McCullough J, Alian A, Sadler JBA, Dempsey N, Laughlin TG, Nguyen HC, Wenzel D, Lalonde MS, Ventimiglia LN, LaJoie D, Iwasa J, Starling T, Padilla-Parra S, Ullman KS, Frost A, Sundquist WI, Martin-Serrano J
To Be Published
- Chmp1b/ist1 copolymer bound to a 60-mer oligonucleotide of ssdna (397 kDa, Complex from Homo sapiens)
- Charged multivesicular body protein 1b (chmp1b) (Complex from Homo sapiens)
- Increased sodium tolerance 1 (ist1) (Complex from Homo sapiens)
- Ist1 homolog (39 kDa, Protein from Homo sapiens)
- Charged multivesicular body protein 1b (22 kDa, Protein from Homo sapiens)
CryoEM reconstruction of membrane-bound ESCRT-III filament composed of CHMP1B+IST1 (right-handed)
Resolution: 3.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 6tz4
Q-score: 0.253
Nguyen HC, Talledge N, McCullough J, Sharma A, Moss 3rd FR, Iwasa JH, Vershinin MD, Sundquist WI, Frost A
Nat Struct Mol Biol (2020) 27 pp. 392-399 [ Pubmed: 32251413 DOI: doi:10.1038/s41594-020-0404-x ]
- Ist1 homolog (21 kDa, Protein from Homo sapiens)
- Charged multivesicular body protein 1b (22 kDa, Protein from Homo sapiens)
- Membrane-bound escrt-iii copolymer filament composed of chmp1b and ist1 (Complex from Homo sapiens)
CryoEM reconstruction of membrane-bound ESCRT-III filament composed of CHMP1B+IST1 (left-handed)
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6tz5
Q-score: 0.258
Nguyen HC, Talledge N, McCullough J, Sharma A, Moss 3rd FR, Iwasa JH, Vershinin MD, Sundquist WI, Frost A
Nat Struct Mol Biol (2020) 27 pp. 392-399 [ Pubmed: 32251413 DOI: doi:10.1038/s41594-020-0404-x ]
- Ist1 homolog (21 kDa, Protein from Homo sapiens)
- Charged multivesicular body protein 1b (22 kDa, Protein from Homo sapiens)
- Membrane-bound escrt-iii copolymer filament composed of chmp1b and ist1 (Complex from Homo sapiens)
CryoEM reconstruction of membrane-bound ESCRT-III filament composed of CHMP1B only
Resolution: 6.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 6tz9
Q-score: 0.186
Nguyen HC, Talledge N, McCullough J, Sharma A, Moss 3rd FR, Iwasa JH, Vershinin MD, Sundquist WI, Frost A
Nat Struct Mol Biol (2020) 27 pp. 392-399 [ Pubmed: 32251413 DOI: doi:10.1038/s41594-020-0404-x ]
CryoEM reconstruction of ESCRT-III filament composed of IST1 NTD R16E K27E double mutant
Resolution: 7.2 Å
EM Method: Helical reconstruction
Fitted PDBs: 6tza
Q-score: 0.189
Nguyen HC, Talledge N, McCullough J, Sharma A, Moss 3rd FR, Iwasa JH, Vershinin MD, Sundquist WI, Frost A
Nat Struct Mol Biol (2020) 27 pp. 392-399 [ Pubmed: 32251413 DOI: doi:10.1038/s41594-020-0404-x ]
Spastin Hexamer in complex with substrate peptide
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6pek
Q-score: 0.323
Han H, Schubert HL, McCullough J, Monroe N, Purdy MD, Yeager M, Sundquist WI, Hill CP
J Biol Chem (2020) 295 pp. 435-443 [ DOI: doi:10.1074/jbc.AC119.009890 Pubmed: 31767681 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Spastin hexamer in complex with substrate peptide (Complex from Homo sapiens)
- Spastin (54 kDa, Protein from Homo sapiens)
- Substrate peptide, tyr-glu-tyr-glu-tyr-glu-tyr-glu (1 kDa, Protein from synthetic construct)
CryoEM reconstruction of IST1-CHMP1B copolymer filament bound to ssDNA at 2.9 Angstrom resolution
Resolution: 2.9 Å
EM Method: Helical reconstruction
Fitted PDBs: 6e8g
Q-score: 0.537
Talledge N, McCullough J, Wenzel DM, Nguyen HC, Lalonde M, Bajorek M, Skalicky JJ, Frost A, Sundquist WI
bioRxiv (2018) [ DOI: doi:10.1101/386532 ]
- Ist1 homolog (40 kDa, Protein from Homo sapiens)
- Charged multivesicular body protein 1b (22 kDa, Protein from Homo sapiens)
- Helical assembly of ist1 and chmp1b protein bound to ssdna (Complex from Homo sapiens)