LPHN3 (ADGRL3) 7TM domain bound to tethered agonist in complex with G protein heterotrimer
Barros-Álvarez X, Nwokonko RM, Vizurraga A, Matzov D, He F, Papasergi-Scott MM, Robertson MJ, Panova O, Yardeni EH, Seven AB, Kwarcinski FE, Su H, Peroto MC, Meyerowitz JG, Shalev-Benami M, Tall GG, Skiniotis G
Nature (2022) 604 pp. 757-762 [ DOI: 10.1038/s41586-022-04575-7 Pubmed: 35418682 ]
Image sets:
GPR56 (ADGRG1) 7TM domain bound to tethered agonist in complex with G protein heterotrimer
Barros-Álvarez X, Nwokonko RM, Vizurraga A, Matzov D, He F, Papasergi-Scott MM, Robertson MJ, Panova O, Yardeni EH, Seven AB, Kwarcinski FE, Su H, Peroto MC, Meyerowitz JG, Shalev-Benami M, Tall GG, Skiniotis G
Nature (2022) 604 pp. 757-762 [ DOI: 10.1038/s41586-022-04575-7 Pubmed: 35418682 ]
Image sets:
RMC-5552 in complex with mTORC1 and FKBP12
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8era
Q-score: 0.42
Burnett GL, Yang YC, Aggen JB, Pitzen J, Gliedt MK, Semko CM, Marquez A, Evans JW, Wang G, Won WS, Tomlinson ACA, Kiss G, Tzitzilonis C, Thottumkara AP, Cregg J, Mellem KT, Choi JS, Lee JC, Zhao Y, Lee BJ, Meyerowitz JG, Knox JE, Jiang J, Wang Z, Wildes D, Wang Z, Singh M, Smith JAM, Gill AL
J Med Chem (2023) 66 pp. 149-169 [ DOI: doi:10.1021/acs.jmedchem.2c01658 Pubmed: 36533617 ]
- Regulatory-associated protein of mtor (149 kDa, Protein from Homo sapiens)
- (3s,5r,6r,7e,9r,10r,12r,14s,15e,17e,19e,21s,23s,26r,27r,30r,34as)-5,9,27-trihydroxy-3-{(2r)-1-[(1s,3r,4r)-4-hydroxy-3-methoxycyclohexyl]propan-2-yl}-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-5,6,9,10,12,13,14,21,22,23,24,25,26,27,32,33,34,34a-octadecahydro-3h-23,27-epoxypyrido[2,1-c][1,4]oxazacyclohentriacontine-1,11,28,29(4h,31h)-tetrone (916 Da, Ligand)
- Peptidyl-prolyl cis-trans isomerase fkbp1a (11 kDa, Protein from Homo sapiens)
- 1-[6-{[(3m)-4-amino-3-(2-amino-1,3-benzoxazol-5-yl)-1h-pyrazolo[3,4-d]pyrimidin-1-yl]methyl}-3,4-dihydroisoquinolin-2(1h)-yl]-3-hydroxypropan-1-one (484 Da, Ligand)
- Serine/threonine-protein kinase mtor (289 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Rmc-5552-mtorc1-fkbp12 (Complex from Homo sapiens)
Single Particle CryoEM of inactive SSTR2 bound to Nb6
Robertson MJ, Papasergi-Scott M, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
bioRxiv (2021) [ DOI: 10.1101/2021.11.02.466983 ]
Image sets:
Single Particle CryoEM of inactive MOR bound to Mb6 and alvimopan
Robertson MJ, Papasergi-Scott M, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
bioRxiv (2021) [ DOI: 10.1101/2021.11.02.466983 ]
Image sets:
Single Particle CryoEM of inactive hNTSR1 bound to Nb6 and SR antagonist
Robertson MJ, Papasergi-Scott M, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
bioRxiv (2021) [ DOI: 10.1101/2021.11.02.466983 ]
Image sets:
Single Particle CryoEM of inactive H2R in complex with Nb6M, NabFab, and anti-fab nanobody
Robertson MJ, Papasergi-Scott M, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
bioRxiv (2021) [ DOI: 10.1101/2021.11.02.466983 ]
Image sets:
CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7ul2
Q-score: 0.645
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- 2-[[1-(7-chloranylquinolin-4-yl)-5-(2,6-dimethoxyphenyl)pyrazol-3-yl]carbonylamino]adamantane-2-carboxylic acid (587 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ntsr1 (Complex from Homo sapiens)
- Complex of ntsr1 and nb6 (Complex from Homo sapiens)
- Neurotensin receptor 1 (47 kDa, Protein from Homo sapiens)
CryoEM Structure of Inactive H2R Bound to Famotidine, Nb6M, and NabFab
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ul3
Q-score: 0.36
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nb6m (Complex from Lama glama)
- Nabfab (Complex from synthetic construct)
- H2r (Complex from Homo sapiens)
- Histamine h2 receptor (44 kDa, Protein from Homo sapiens)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nanobody 6m (14 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Complex of h2r, nb6m, and nabfab (Complex from Homo sapiens)
- Famotidine (339 Da, Ligand)
CryoEM Structure of Inactive MOR Bound to Alvimopan and Mb6
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ul4
Q-score: 0.563
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Mb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- Complex of mor and nb6 (Complex from Mus musculus)
- Mor (Complex from Mus musculus)
- N-[(2s)-2-{[(3r,4r)-4-(3-hydroxyphenyl)-3,4-dimethylpiperidin-1-yl]methyl}-3-phenylpropanoyl]glycine (424 Da, Ligand)
- Mu-type opioid receptor (47 kDa, Protein from Mus musculus)
- Megabody 6 (55 kDa, Protein from synthetic construct)