CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7ul2
Q-score: 0.645
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- 2-[[1-(7-chloranylquinolin-4-yl)-5-(2,6-dimethoxyphenyl)pyrazol-3-yl]carbonylamino]adamantane-2-carboxylic acid (587 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ntsr1 (Complex from Homo sapiens)
- Complex of ntsr1 and nb6 (Complex from Homo sapiens)
- Neurotensin receptor 1 (47 kDa, Protein from Homo sapiens)
CryoEM Structure of Inactive H2R Bound to Famotidine, Nb6M, and NabFab
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ul3
Q-score: 0.36
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nb6m (Complex from Lama glama)
- Nabfab (Complex from synthetic construct)
- H2r (Complex from Homo sapiens)
- Histamine h2 receptor (44 kDa, Protein from Homo sapiens)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nanobody 6m (14 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Complex of h2r, nb6m, and nabfab (Complex from Homo sapiens)
- Famotidine (339 Da, Ligand)
CryoEM Structure of Inactive MOR Bound to Alvimopan and Mb6
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ul4
Q-score: 0.563
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Mb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- Complex of mor and nb6 (Complex from Mus musculus)
- Mor (Complex from Mus musculus)
- N-[(2s)-2-{[(3r,4r)-4-(3-hydroxyphenyl)-3,4-dimethylpiperidin-1-yl]methyl}-3-phenylpropanoyl]glycine (424 Da, Ligand)
- Mu-type opioid receptor (47 kDa, Protein from Mus musculus)
- Megabody 6 (55 kDa, Protein from synthetic construct)
CryoEM Structure of Inactive SSTR2 bound to Nb6
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ul5
Q-score: 0.562
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Complex of sstr2 and nb6 (Complex from Homo sapiens)
- Somatostatin receptor type 2 (46 kDa, Protein from Homo sapiens)
- Sstr2 (Complex from Homo sapiens)
LPHN3 (ADGRL3) 7TM domain bound to tethered agonist in complex with G protein heterotrimer
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7sf7
Q-score: 0.479
Barros-Alvarez X, Nwokonko RM, Vizurraga A, Matzov D, He F, Papasergi-Scott MM, Robertson MJ, Panova O, Yardeni EH, Seven AB, Kwarcinski FE, Su H, Peroto MC, Meyerowitz JG, Shalev-Benami M, Tall GG, Skiniotis G
Nature (2022) 604 pp. 757-762 [ Pubmed: 35418682 DOI: doi:10.1038/s41586-022-04575-7 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Latrophilin 3 (adgrl3) 7tm domain bound to tethered agonist in complex with mini-g13 protein (Complex from Homo sapiens)
- Isoform 1 of adhesion g protein-coupled receptor l3 (34 kDa, Protein from Homo sapiens)
GPR56 (ADGRG1) 7TM domain bound to tethered agonist in complex with G protein heterotrimer
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sf8
Q-score: 0.544
Barros-Alvarez X, Nwokonko RM, Vizurraga A, Matzov D, He F, Papasergi-Scott MM, Robertson MJ, Panova O, Yardeni EH, Seven AB, Kwarcinski FE, Su H, Peroto MC, Meyerowitz JG, Shalev-Benami M, Tall GG, Skiniotis G
Nature (2022) 604 pp. 757-762 [ Pubmed: 35418682 DOI: doi:10.1038/s41586-022-04575-7 ]
- Isoform 2 of adhesion g-protein coupled receptor g1 (34 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
- Gpr56 (adgrg1) 7tm domain bound to tethered agonist in complex with mini-g13 protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
Oxytocin receptor (OTR) bound to oxytocin in complex with a heterotrimeric Gq protein
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ryc
Q-score: 0.551
Meyerowitz JG, Robertson MJ, Barros-Alvarez X, Panova O, Nwokonko RM, Gao Y, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 274-281 [ Pubmed: 35241813 DOI: doi:10.1038/s41594-022-00728-4 ]
- Magnesium ion (24 Da, Ligand)
- Oxytocin receptor (Complex from Homo sapiens)
- Oxytocin receptor signaling complex with oxytocin and minigq and an scfv (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, guanine nucleotide-binding protein g(i) subunit alpha-2,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (36 kDa, Protein from Homo sapiens)
- Oxytocin (Complex from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Minigq (Complex from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Oxytocin receptor (49 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Oxytocin (1 kDa, Protein from Homo sapiens)
CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t10
Q-score: 0.615
Robertson MJ, Meyerowitz JG, Panova O, Borrelli K, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 210-217 [ DOI: doi:10.1038/s41594-022-00727-5 Pubmed: 35210615 ]
- Somatostatin receptor 2 in complex with somatostatin-14 and gi3. (Complex)
- Guanine nucleotide-binding protein g(i) subunit alpha-3 (40 kDa, Protein from Homo sapiens)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2,kappa-type opioid receptor (45 kDa, Protein from Homo sapiens)
CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7t11
Q-score: 0.53
Robertson MJ, Meyerowitz JG, Panova O, Borrelli K, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 210-217 [ DOI: doi:10.1038/s41594-022-00727-5 Pubmed: 35210615 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-3 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Octreotide (1 kDa, Protein from synthetic construct)
- Somatostatin receptor 2 in complex with octreotide and gi3. (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2,kappa-type opioid receptor (45 kDa, Protein from Homo sapiens)
CryoEM Structure of Full-Length mGlu2 in Inactive-State Bound to Antagonist LY341495
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 7mtq
Q-score: 0.39
Seven AB, Barros-Alvarez X, de Lapeyriere M, Papasergi-Scott MM, Robertson MJ, Zhang C, Nwokonko RM, Gao Y, Meyerowitz JG, Rocher JP, Schelshorn D, Kobilka BK, Mathiesen JM, Skiniotis G
Nature (2021) 595 pp. 450-454 [ DOI: doi:10.1038/s41586-021-03680-3 Pubmed: 34194039 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Metabotropic glutamate receptor 2 (93 kDa, Protein from Homo sapiens)
- 2-[(1s,2s)-2-carboxycyclopropyl]-3-(9h-xanthen-9-yl)-d-alanine (353 Da, Ligand)
- Full-length mglu2 in inactive-state (191 kDa, Complex from Homo sapiens)