Kalium channelrhodopsin 1 C110A mutant from Hyphochytrium catenoides, Dark State
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 9cdc
Q-score: 0.465
Morizumi T, Kim K, Li H, Nag P, Dogon T, Sineshchekov OA, Wang Y, Brown LS, Hwang S, Sun H, Bondar AN, Schapiro I, Govorunova EG, Spudich JL, Ernst OP
Nat Commun (2025) 16 pp. 1283-1283 [ DOI: doi:10.1038/s41467-025-56491-9 Pubmed: 39900567 ]
- Water (18 Da, Ligand)
- Retinal (284 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Kalium channelrhodopsin 1 c110a (30 kDa, Protein from Hyphochytrium catenoides)
- Cholesterol (386 Da, Ligand)
- Kalium channelrhodopin 1 c110a reconstituted in peptidisc, dark state (91 MDa, Complex from Hyphochytrium catenoides)
Kalium channelrhodopsin 1 C110A mutant from Hyphochytrium catenoides, Laser-Flash-Illuminated
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 9cdd
Q-score: 0.432
Morizumi T, Kim K, Li H, Nag P, Dogon T, Sineshchekov OA, Wang Y, Brown LS, Hwang S, Sun H, Bondar AN, Schapiro I, Govorunova EG, Spudich JL, Ernst OP
Nat Commun (2025) 16 pp. 1283-1283 [ DOI: doi:10.1038/s41467-025-56491-9 Pubmed: 39900567 ]
- Water (18 Da, Ligand)
- Retinal (284 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Kalium channelrhodopin 1 c110a reconstituted in peptidisc, laser-flash-illuminated (91 MDa, Complex from Hyphochytrium catenoides)
- Kalium channelrhodopsin 1 (30 kDa, Protein from Hyphochytrium catenoides)
- Cholesterol (386 Da, Ligand)
Kalium channelrhodopsin 1 C110A mutant from Hyphochytrium catenoides, Continuous Illumination State
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 9cde
Q-score: 0.395
Morizumi T, Kim K, Li H, Nag P, Dogon T, Sineshchekov OA, Wang Y, Brown LS, Hwang S, Sun H, Bondar AN, Schapiro I, Govorunova EG, Spudich JL, Ernst OP
Nat Commun (2025) 16 pp. 1283-1283 [ DOI: doi:10.1038/s41467-025-56491-9 Pubmed: 39900567 ]
- Water (18 Da, Ligand)
- Retinal (284 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Kalium channelrhodopin 1 c110a reconstituted in peptidisc, continuous illumination state (91 MDa, Complex from Hyphochytrium catenoides)
- Kalium channelrhodopsin 1 c110a - continuous illumination state (30 kDa, Protein from Hyphochytrium catenoides)
- Cholesterol (386 Da, Ligand)
Kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) embedded in peptidisc
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8gi8
Q-score: 0.558
Morizumi T, Kim K, Li H, Govorunova EG, Sineshchekov OA, Wang Y, Zheng L, Bertalan E, Bondar AN, Askari A, Brown LS, Spudich JL, Ernst OP
Nat Commun (2023) 14 pp. 4365-4365 [ DOI: doi:10.1038/s41467-023-40041-2 Pubmed: 37474513 ]
- Water (18 Da, Ligand)
- Retinal (284 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Kalium channelrhodopsin 1 (30 kDa, Protein from Hyphochytrium catenoides)
- Sodium ion (22 Da, Ligand)
- Kalium channelrhodopsin 1 trimer reconstituted into nanodiscs (30 kDa, Complex from Hyphochytrium catenoides)
- Cholesterol (386 Da, Ligand)
Cation channelrhodopsin from Hyphochytrium catenoides (HcCCR) embedded in peptidisc
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8gi9
Q-score: 0.578
Morizumi T, Kim K, Li H, Govorunova EG, Sineshchekov OA, Wang Y, Zheng L, Bertalan E, Bondar AN, Askari A, Brown LS, Spudich JL, Ernst OP
Nat Commun (2023) 14 pp. 4365-4365 [ DOI: doi:10.1038/s41467-023-40041-2 Pubmed: 37474513 ]
- Water (18 Da, Ligand)
- Retinal (284 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Cation channelrhodopsin (30 kDa, Protein from Hyphochytrium catenoides)
- Cation channelrhodopsin trimer reconstituted in peptidisc (30 kDa, Complex from Hyphochytrium catenoides)
- Cholesterol (386 Da, Ligand)