Cryo-EM structure of human RNA Polymerase I in elongation state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7ob9
Q-score: 0.519
Misiaszek AD, Girbig M, Grotsch H, Baudin F, Murciano B, Lafita A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 997-1008 [ DOI: doi:10.1038/s41594-021-00693-4 Pubmed: 34887565 ]
- Rna polymerase i (Complex from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa49 (47 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Rna and dna (Complex from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa34 (55 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa1 (195 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Homo sapiens)
- Rna (9 kDa, RNA from Homo sapiens)
- Dna template strand (13 kDa, DNA from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase i subunit rpa43 (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Dna non-template strand (13 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Rna polymerase i (Complex)
- Dna-directed rna polymerase i subunit rpa2 (128 kDa, Protein from Homo sapiens)
Cryo-EM structure of human RNA Polymerase III elongation complex 1
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ae1
Q-score: 0.48
Girbig M, Misiaszek AD, Vorlander MK, Lafita A, Grotsch H, Baudin F, Bateman A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 210-219 [ DOI: doi:10.1038/s41594-020-00555-5 Pubmed: 33558764 ]
- Rna (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Homo sapiens)
- Human rna polymerase iii elongation complex 1 (776 kDa, Complex)
- Dna-directed rna polymerase iii subunit rpc9 (16 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc6 (35 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc5 (80 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc1 (155 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc7 (25 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc4 (44 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Template-dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc8 (22 kDa, Protein from Homo sapiens)
- Rna, dna (Complex from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc2 (127 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc3 (60 kDa, Protein from Homo sapiens)
- Human rna polymerase (Complex from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Non-template dna (14 kDa, DNA from synthetic construct)
Cryo-EM structure of human RNA Polymerase III elongation complex 3
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ae3
Q-score: 0.373
Girbig M, Misiaszek AD, Vorlander MK, Lafita A, Grotsch H, Baudin F, Bateman A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 210-219 [ DOI: doi:10.1038/s41594-020-00555-5 Pubmed: 33558764 ]
- Rna (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc9 (16 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Human rna polymerase iii elongation complex (Complex from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc6 (35 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc5 (80 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc1 (155 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc7 (25 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc4 (44 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Template dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc8 (22 kDa, Protein from Homo sapiens)
- Rna, dna (Complex from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc2 (127 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc3 (60 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Non-template dna (14 kDa, DNA from synthetic construct)
- Human rna polymerase iii elongation complex 3 (776 kDa, Complex)
Cryo-EM structure of human RNA Polymerase III elongation complex 2
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7aea
Q-score: 0.409
Girbig M, Misiaszek AD, Vorlander MK, Lafita A, Grotsch H, Baudin F, Bateman A, Muller CW
Nat Struct Mol Biol (2021) 28 pp. 210-219 [ DOI: doi:10.1038/s41594-020-00555-5 Pubmed: 33558764 ]
- Rna (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc9 (16 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (17 kDa, Protein from Homo sapiens)
- Human rna polymerase iii elongation complex (Complex from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc6 (35 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc5 (80 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac1 (39 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc1 (155 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (24 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc7 (25 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc4 (44 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (7 kDa, Protein from Homo sapiens)
- Template dna (14 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase iii subunit rpc8 (22 kDa, Protein from Homo sapiens)
- Rna, dna (Complex from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase iii subunit rpc2 (127 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase iii subunit rpc3 (60 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerases i and iii subunit rpac2 (15 kDa, Protein from Homo sapiens)
- Human rna polymerase iii elongation complex (776 kDa, Complex)
- Iron/sulfur cluster (351 Da, Ligand)
- Non-template dna (14 kDa, DNA from synthetic construct)
tRNA-bound Elongator catalytic subcomplex, tRNA-Elp123 lobe.
Dauden MI, Jaciuk M, Weis F, Lin TY, Kleindienst C, Abbassi NEH, Khatter H, Krutyholowa R, Breunig KD, Kosinski J, Muller CW, Glatt S
Sci Adv (2019) 5 pp. eaaw2326-eaaw2326 [ DOI: doi:10.1126/sciadv.aaw2326 Pubmed: 31309145 ]
- Trna-bound elongator catalytic subcomplex lobe, trna-elp123 lobe (643 kDa, Complex from Saccharomyces cerevisiae)
- Elongator complex protein 3, elp3 (Protein from Saccharomyces cerevisiae)
- Elongator complex protein 2, elp2 (Protein from Saccharomyces cerevisiae)
- Elongator complex protein 1, elp1 (Protein from Saccharomyces cerevisiae)
- Trna ala ugc (RNA from Saccharomyces cerevisiae)
2tRNAs-bound Elongator catalytic subcomplex, 2tRNA-Elp123.
Dauden MI, Jaciuk M, Weis F, Lin TY, Kleindienst C, Abbassi NEH, Khatter H, Krutyholowa R, Breunig KD, Kosinski J, Muller CW, Glatt S
Sci Adv (2019) 5 pp. eaaw2326-eaaw2326 [ Pubmed: 31309145 DOI: doi:10.1126/sciadv.aaw2326 ]
- Elongator complex protein 1, elp1 (Protein from Saccharomyces cerevisiae)
- Trna ala ugc (RNA from Saccharomyces cerevisiae)
- 2trnas-bound elongator catalytic subcomplex, 2trnas-elp123. (665 kDa, Complex from Saccharomyces cerevisiae)
- Elongator complex protein 3, elp3 (Protein from Saccharomyces cerevisiae)
- Elongator complex protein 2, elp2 (Protein from Saccharomyces cerevisiae)
Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 6hko
Q-score: 0.478
Tafur L, Sadian Y, Hanske J, Wetzel R, Weis F, Muller CW
eLife (2019) 8 [ DOI: doi:10.7554/eLife.43204 Pubmed: 30913026 ]
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa49 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa34 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast rna polymerase i elongation complex bound to nucleotide analog gmpcpp (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Non-template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna (6 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast RNA polymerase I* elongation complex bound to nucleotide analog GMPCPP
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 6hlq
Q-score: 0.524
Tafur L, Sadian Y, Hanske J, Wetzel R, Weis F, Muller CW
eLife (2019) 8 [ DOI: doi:10.7554/eLife.43204 Pubmed: 30913026 ]
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast rna polymerase i* elongation complex bound to nucleotide analog gmpcpp (Complex)
- Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Non-template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna (6 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Yeast rna polymerase i* (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP (core focused)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 6hlr
Q-score: 0.512
Tafur L, Sadian Y, Hanske J, Wetzel R, Weis F, Muller CW
eLife (2019) 8 [ DOI: doi:10.7554/eLife.43204 Pubmed: 30913026 ]
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast rna polymerase i elongation complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast rna polymerase i elongation complex bound to nucleotide analog gmpcpp (core focused) (Complex)
- Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Non-template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna (6 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))