chicken KNL2 in complex with the CENP-A nucleosome
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 7y7i
Q-score: 0.454
Jiang H, Ariyoshi M, Watanabe R, Makino F, Namba K, Fukagawa T
bioRxiv (2022) [ DOI: doi:10.1101/2022.06.24.497480 ]
- Myb-like domain-containing protein (4 kDa, Protein from Gallus gallus)
- The cenp-c like motif of chicken knl2 in complex with the cenp-a nucleosome (200 kDa, Complex from Gallus gallus)
- Dna (Complex)
- Chimeric cenp-a (Complex from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Chains: j (44 kDa, DNA from synthetic construct)
- Nucleosome (Complex from synthetic construct)
- Histone h3.1,histone h3-like centromeric protein a (16 kDa, Protein from Gallus gallus)
- Chains: i (43 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (14 kDa, Protein from Homo sapiens)
- Myb-like domain-containing protein (Complex)
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
EMBO J (2021) 40 [ Pubmed: 33463726 DOI: 10.15252/embj.2020105671 ]
Image sets:
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
EMBO J (2021) 40 [ Pubmed: 33463726 DOI: 10.15252/embj.2020105671 ]
Image sets:
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
EMBO J (2021) 40 [ Pubmed: 33463726 DOI: 10.15252/embj.2020105671 ]
Image sets:
The cryo-EM density map of CENP-A nucleosome in complex with CENP-C C-terminal domain
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
EMBO J (2021) 40 pp. e105671-e105671 [ Pubmed: 33463726 DOI: doi:10.15252/embj.2020105671 ]
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
EMBO J (2021) 40 pp. e105671-e105671 [ Pubmed: 33463726 DOI: doi:10.15252/embj.2020105671 ]
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7bxt
Q-score: 0.387
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
EMBO J (2021) 40 pp. e105671-e105671 [ Pubmed: 33463726 DOI: doi:10.15252/embj.2020105671 ]
- Maltodextrin-binding protein,centromere protein n (71 kDa, Protein from Gallus gallus)
- Dna (Complex)
- Maltodextrin-binding protein,centromere protein n (Complex)
- Histone h2a type 1-b/e (Complex from Homo sapiens)
- Histone h4 (Complex from Homo sapiens)
- Histone h3,histone h3-like centromeric protein a (16 kDa, Protein from Gallus gallus)
- Cenp-c (Complex)
- Histone h2b type 2-e (Complex from Escherichia coli (strain B / BL21-DE3))
- Histone h3,histone h3-like centromeric protein a (Complex from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h2b type 2-e (14 kDa, Protein from Homo sapiens)
- Cenp-a nucleosome in complex with cenp-c peptide and cnep-n n-terminal domain (330 kDa, Complex from Ectocarpus siliculosus)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Cenp-c (4 kDa, Protein from Gallus gallus)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
The cryo-EM structure of CENP-A nucleosome in complex with the phosphorylated CENP-C
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7by0
Q-score: 0.297
Ariyoshi M, Makino F, Watanabe R, Nakagawa R, Kato T, Namba K, Arimura Y, Fujita R, Kurumizaka H, Okumura EI, Hara M, Fukagawa T
EMBO J (2021) 40 pp. e105671-e105671 [ Pubmed: 33463726 DOI: doi:10.15252/embj.2020105671 ]
- Dna (145-mer) (Complex)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h4 (Complex from Homo sapiens)
- Histone h2a type 1-b/e (Complex from Homo sapiens)
- Histone h3.2,histone h3-like centromeric protein a (16 kDa, Protein from Gallus gallus)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Cenp-a nucleosome in complex with cenp-c c-terminal and cnep-n n-terminal domain (330 kDa, Complex from Gallus gallus)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Histone h2b type 1-j (Complex from Gallus gallus)
- Dna (145-mer) (44 kDa, DNA from synthetic construct)
- Histone h3,histone h3-like centromeric protein a (Complex from Homo sapiens)
- Maltose binding protein tag, linker,cenp-c (73 kDa, Protein from Gallus gallus)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Maltodextrin-binding protein,linker peptide,cenp-c (Complex)