Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8ssz
Q-score: 0.537
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- Water (18 Da, Ligand)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- A complex of three fab fragments with the 2alpha3beta stoichiometry of the full-length human alpha4beta2 nicotinic receptor bound to acetylcholine and calcium (Complex from Homo sapiens)
- Neuronal acetylcholine receptor subunit alpha-4 (67 kDa, Protein from Homo sapiens)
- N-octane (114 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (55 kDa, Protein from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8st0
Q-score: 0.567
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- A complex of three fab fragments with the 2alpha3beta stoichiometry of the full-length human alpha4beta2 nicotinic receptor bound to acetylcholine (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit alpha-4 (67 kDa, Protein from Homo sapiens)
- N-octane (114 Da, Ligand)
- Neuronal acetylcholine receptor subunit beta-2 (55 kDa, Protein from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8st1
Q-score: 0.496
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- Water (18 Da, Ligand)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- A complex of two fab fragments with the 3alpha2beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine and calcium (Complex from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8st2
Q-score: 0.545
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- Water (18 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
- A complex of two fab fragments with the 3alpha2beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine (Complex from Homo sapiens)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8st3
Q-score: 0.533
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- Water (18 Da, Ligand)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- A complex of three fab fragments with the 2alpha3beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine and calcium (Complex from Homo sapiens)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
Resolution: 2.35 Å
EM Method: Single-particle
Fitted PDBs: 8st4
Q-score: 0.597
Mazzaferro S, Kang G, Natarajan K, Hibbs RE, Sine SM
Br.J.Pharmacol. (2024) 181 pp. 1973-1992 [ DOI: doi:10.1111/bph.16321 Pubmed: 38454578 ]
- Water (18 Da, Ligand)
- A complex of three fab fragments with the 2alpha3beta stoichiometry of human alpha4beta2 nicotinic receptor bound to acetylcholine (Complex from Homo sapiens)
- Igg1 heavy chain (51 kDa, Protein from Mus musculus)
- Neuronal acetylcholine receptor subunit beta-2 (46 kDa, Protein from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Igg1 kappa light chain (26 kDa, Protein from Mus musculus)
- Acetylcholine (146 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Neuronal acetylcholine receptor subunit alpha-4 (44 kDa, Protein from Homo sapiens)
CryoEm structure of SARS-CoV-2 spike protein (S-6P, 1-up) in complex with sybodies (Sb45)
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 7n0g
Q-score: 0.397
Ahmad J, Jiang J, Boyd LF, Zeher A, Huang R, Xia D, Natarajan K, Margulies DH
J Biol Chem (2021) 297 pp. 101202-101202 [ Pubmed: 34537245 DOI: doi:10.1016/j.jbc.2021.101202 ]
- Spike protein (s-6p) in complex with synthetic nanobody (sb45) (600 kDa, Complex from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Synthetic nanobody (sybody), sb45 (13 kDa, Protein from synthetic construct)
CryoEM structure of SARS-CoV-2 spike protein (S-6P, 2-up) in complex with sybodies (Sb45)
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 7n0h
Q-score: 0.356
Ahmad J, Jiang J, Boyd LF, Zeher A, Huang R, Xia D, Natarajan K, Margulies DH
J Biol Chem (2021) 297 pp. 101202-101202 [ Pubmed: 34537245 DOI: doi:10.1016/j.jbc.2021.101202 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Synthetic nanobody (sb45) (13 kDa, Protein from synthetic construct)
- Spike protein (s-6p, 2-up) in complex with synthetic nanobody (sb45) (600 kDa, Complex from synthetic construct)