Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 8thi
Q-score: 0.411
Currie MJ, Davies JS, Scalise M, Gulati A, Wright JD, Newton-Vesty MC, Abeysekera GS, Subramanian R, Wahlgren WY, Friemann R, Allison JR, Mace PD, Griffin MDW, Demeler B, Wakatsuki S, Drew D, Indiveri C, Dobson RCJ, North RA
eLife (2024) 12 [ Pubmed: 38349818 DOI: doi:10.7554/eLife.92307 ]
- Sodium ion (22 Da, Ligand)
- Hisiaqm transporter protein solubilised in amphipol a8-35 (Complex from Haemophilus influenzae Rd KW20)
- Sialic acid trap transporter permease protein siat (72 kDa, Protein from Haemophilus influenzae Rd KW20)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8thj
Q-score: 0.52
Currie MJ, Davies JS, Scalise M, Gulati A, Wright JD, Newton-Vesty MC, Abeysekera GS, Subramanian R, Wahlgren WY, Friemann R, Allison JR, Mace PD, Griffin MDW, Demeler B, Wakatsuki S, Drew D, Indiveri C, Dobson RCJ, North RA
eLife (2024) 12 [ Pubmed: 38349818 DOI: doi:10.7554/eLife.92307 ]
- Hisiaqm transporter protein solubilised in amphipol a8-35 (Complex from Haemophilus influenzae Rd KW20)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Sialic acid trap transporter permease protein siat (72 kDa, Protein from Haemophilus influenzae Rd KW20)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8b01
Q-score: 0.539
Davies JS, Currie MJ, North RA, Scalise M, Wright JD, Copping JM, Remus DM, Gulati A, Morado DR, Jamieson SA, Newton-Vesty MC, Abeysekera GS, Ramaswamy S, Friemann R, Wakatsuki S, Allison JR, Indiveri C, Drew D, Mace PD, Dobson RCJ
Nat Commun (2023) 14 pp. 1120-1120 [ Pubmed: 36849793 DOI: doi:10.1038/s41467-023-36590-1 ]
- Hexane (86 Da, Ligand)
- Putative trap-type c4-dicarboxylate transport system, large permease component (45 kDa, Protein from Photobacterium profundum SS9)
- Megabody (Complex from Helicobacter pylori)
- Megabody c7hopq (54 kDa, Protein from synthetic construct)
- N-octane (114 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Siaqm (Complex from Photobacterium profundum)
- Decane (142 Da, Ligand)
- Tridecane (184 Da, Ligand)
- Putative trap-type c4-dicarboxylate transport system, small permease component (19 kDa, Protein from Photobacterium profundum SS9)
- Siaqm with megabody (Complex)
- Docosane (310 Da, Ligand)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 7qha
Q-score: 0.48
Davies JS, Currie MJ, North RA, Scalise M, Wright JD, Copping JM, Remus DM, Gulati A, Morado DR, Jamieson SA, Newton-Vesty MC, Abeysekera GS, Ramaswamy S, Friemann R, Wakatsuki S, Allison JR, Indiveri C, Drew D, Mace PD, Dobson RCJ
Nat Commun (2023) 14 pp. 1120-1120 [ Pubmed: 36849793 DOI: doi:10.1038/s41467-023-36590-1 ]
- Putative trap-type c4-dicarboxylate transport system, large permease component (45 kDa, Protein from Photobacterium profundum SS9)
- Megabody (Complex from Helicobacter pylori)
- Megabody c7hopq (54 kDa, Protein from synthetic construct)
- Sodium ion (22 Da, Ligand)
- Siaqm (Complex from Photobacterium profundum)
- Putative trap-type c4-dicarboxylate transport system, small permease component (19 kDa, Protein from Photobacterium profundum SS9)
- Siaqm with megabody (Complex)
CryoEM single particle dataset for NanR dimer-DNA hetero-complex.
Venugopal H, Horne CR, Panjikar S, Wood DM, Henrickson A, Brookes E, North RA, Murphy JM, Friemann R, Griffin MDW, Ramm G, Demeler B, Dobson RCJ
Nat Commun (2021) 12 [ Pubmed: 33790291 DOI: 10.1038/s41467-021-22253-6 ]
Image sets:
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6wfq
Q-score: 0.256
Horne CR, Venugopal H, Panjikar S, Wood DM, Henrickson A, Brookes E, North RA, Murphy JM, Friemann R, Griffin MDW, Ramm G, Demeler B, Dobson RCJ
Nat Commun (2021) 12 pp. 1988-1988 [ DOI: doi:10.1038/s41467-021-22253-6 Pubmed: 33790291 ]
- Hth-type transcriptional repressor nanr (29 kDa, Protein from Escherichia coli)
- Dna (5'-d(p*gp*gp*tp*ap*tp*ap*ap*cp*ap*gp*gp*tp*ap*tp*a)-3') (4 kDa, DNA from synthetic construct)
- Nanr-dna hetero-complex (72 kDa, Complex from Escherichia coli)
- Dna (5'-d(p*tp*ap*tp*ap*cp*cp*tp*gp*tp*tp*ap*tp*ap*cp*c)-3') (4 kDa, DNA from synthetic construct)
Hexameric NanR-DNA hetero-complex
Resolution: 8.3 Å
EM Method: Single-particle
Fitted PDBs: 6wg7
Q-score: 0.062
Horne CR, Venugopal H, Panjikar S, Wood DM, Henrickson A, Brookes E, North RA, Murphy JM, Friemann R, Griffin MDW, Ramm G, Demeler B, Dobson RCJ
Nat Commun (2021) 12 pp. 1988-1988 [ DOI: doi:10.1038/s41467-021-22253-6 Pubmed: 33790291 ]
- Hth-type transcriptional repressor nanr (29 kDa, Protein from Escherichia coli)
- Dna (35-mer) (10 kDa, DNA from synthetic construct)
- Hexameric nanr-dna hetero-complex (198 kDa, Complex from Escherichia coli)
- Dna (35-mer) (10 kDa, DNA from synthetic construct)