Vanadate-trapped BSEP in nanodiscs
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8pmj
Q-score: 0.59
Liu H, Irobalieva RN, Kowal J, Ni D, Nosol K, Bang-Sorensen R, Lancien L, Stahlberg H, Stieger B, Locher KP
Nat Commun (2023) 14 pp. 7296-7296 [ Pubmed: 37949847 DOI: doi:10.1038/s41467-023-43109-1 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Bile salt export pump (146 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Vanadate-trapped bsep in nanodiscs (146 kDa, Complex from Homo sapiens)
- Vanadate ion (114 Da, Ligand)
Human bile salt export pump (BSEP) in complex with inhibitor GBM in nanodiscs
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8pm6
Q-score: 0.428
Liu H, Irobalieva RN, Kowal J, Ni D, Nosol K, Bang-Sorensen R, Lancien L, Stahlberg H, Stieger B, Locher KP
Nat Commun (2023) 14 pp. 7296-7296 [ Pubmed: 37949847 DOI: doi:10.1038/s41467-023-43109-1 ]
- Cholesterol (386 Da, Ligand)
- Bile salt export pump (146 kDa, Protein from Homo sapiens)
- The complex of bsep with inhibitor gbm (146 kDa, Complex from Homo sapiens)
- 5-chloro-n-(2-{4-[(cyclohexylcarbamoyl)sulfamoyl]phenyl}ethyl)-2-methoxybenzamide (494 Da, Ligand)
Nucleotide-bound BSEP in nanodiscs
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8pmd
Q-score: 0.519
Liu H, Irobalieva RN, Kowal J, Ni D, Nosol K, Bang-Sorensen R, Lancien L, Stahlberg H, Stieger B, Locher KP
Nat Commun (2023) 14 pp. 7296-7296 [ Pubmed: 37949847 DOI: doi:10.1038/s41467-023-43109-1 ]
- Water (18 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nucleotide-bound bsep in nanodiscs (146 kDa, Complex from Homo sapiens)
- Bile salt export pump (146 kDa, Protein from Homo sapiens)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 8pg0
Q-score: 0.528
Ciuta AD, Nosol K, Kowal J, Mukherjee S, Ramirez AS, Stieger B, Kossiakoff AA, Locher KP
Nat Commun (2023) 14 pp. 5774-5774 [ DOI: doi:10.1038/s41467-023-41552-8 Pubmed: 37723174 ]
- Bicarbonate ion (61 Da, Ligand)
- Solute carrier organic anion transporter family member 1b3 (77 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Fab19 (light chain, variable region) (23 kDa, Protein from Homo sapiens)
- Human oatp1b3 (180 kDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fab19 (heavy chain, variable region) (25 kDa, Protein from Homo sapiens)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8phw
Q-score: 0.38
Ciuta AD, Nosol K, Kowal J, Mukherjee S, Ramirez AS, Stieger B, Kossiakoff AA, Locher KP
Nat Commun (2023) 14 pp. 5774-5774 [ DOI: doi:10.1038/s41467-023-41552-8 Pubmed: 37723174 ]
- Human oatp1b1 (180 kDa, Complex)
- Solute carrier organic anion transporter family member 1b1 (76 kDa, Protein from Homo sapiens)
- Fab18 (light chain, variable region) (23 kDa, Protein from Homo sapiens)
- Solute carrier organic anion transporter family member 1b1 (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Fab18 (variable region) (Complex from Homo sapiens)
- Fab18 (heavy chain, variable region) (24 kDa, Protein from Homo sapiens)
- Estrone 3-sulfate (350 Da, Ligand)
Structure of the human sodium/bile acid cotransporter (NTCP) in complex with Fab and nanobody
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 7zyi
Q-score: 0.502
Liu H, Irobalieva RN, Bang-Sorensen R, Nosol K, Mukherjee S, Agrawal P, Stieger B, Kossiakoff AA, Locher KP
Cell Res (2022) 32 pp. 773-776 [ Pubmed: 35726088 DOI: doi:10.1038/s41422-022-00680-4 ]
- Water (18 Da, Ligand)
- Nanobody (13 kDa, Protein from Lama glama)
- Glycochenodeoxycholic acid (449 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Sodium/bile acid cotransporter (38 kDa, Protein from Homo sapiens)
- Light chain of fab (23 kDa, Protein from Mus musculus)
- Heavy chain of fab (25 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Sodium/bile acid cotransporter (ntcp) in complex with fab and nanobody (110 kDa, Complex from Homo sapiens)
CryoEM Structure of the, UND-PP bound, WaaL O-Antigen Ligase
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: 10.1038/s41586-022-04555-x Pubmed: 35388216 ]
Image sets:
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its ligand bound state
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7tpg
Q-score: 0.497
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Geranyl diphosphate (314 Da, Ligand)
- Ligand bound cmwaal with fab fragment bound (Complex)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its apo state
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7tpj
Q-score: 0.431
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Apo cmwaal with fab fragment bound (Complex)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7niu
Q-score: 0.333
Nosol K, Bang-Sorensen R, Irobalieva RN, Erramilli SK, Stieger B, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34385322 DOI: doi:10.1073/pnas.2106702118 ]
- 4b1 fab-fragment heavy chain (25 kDa, Protein from Homo sapiens)
- Qa2 fab-fragment light chain (23 kDa, Protein from Homo sapiens)
- 4b1 fab-fragment: light and heavy chain (Complex from Homo sapiens)
- Isoform 2 of phosphatidylcholine translocator abcb4 (140 kDa, Protein from Homo sapiens)
- Nanodisc reconstituted human abcb4 in complex with 4b1-fab and qa2-fab (apo-inward-open conformation) (340 kDa, Complex)
- Qa2 fab-fragment: light and heavy chain (Complex from Homo sapiens)
- Qa2 fab-fragment heavy chain (24 kDa, Protein from Homo sapiens)
- Phosphatidylcholine translocator abcb4 (Complex from Homo sapiens)
- 4b1 fab-fragment light chain (23 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)