CryoEM Structure of the, UND-PP bound, WaaL O-Antigen Ligase
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: 10.1038/s41586-022-04555-x Pubmed: 35388216 ]
Image sets:
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its ligand bound state
Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 7tpg
Q-score: 0.497
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Geranyl diphosphate (314 Da, Ligand)
- Ligand bound cmwaal with fab fragment bound (Complex)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
Single-Particle Cryo-EM Structure of the WaaL O-antigen ligase in its apo state
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7tpj
Q-score: 0.431
Ashraf KU, Nygaard R, Vickery ON, Erramilli SK, Herrera CM, McConville TH, Petrou VI, Giacometti SI, Dufrisne MB, Nosol K, Zinkle AP, Graham CLB, Loukeris M, Kloss B, Skorupinska-Tudek K, Swiezewska E, Roper DI, Clarke OB, Uhlemann AC, Kossiakoff AA, Trent MS, Stansfeld PJ, Mancia F
Nature (2022) 604 pp. 371-376 [ DOI: doi:10.1038/s41586-022-04555-x Pubmed: 35388216 ]
- Fab heavy (h) chain (25 kDa, Protein from Homo sapiens)
- Putative cell surface polysaccharide polymerase/ligase (44 kDa, Protein from Cupriavidus metallidurans)
- Fab light (l) chain (23 kDa, Protein from Homo sapiens)
- Apo cmwaal with fab fragment bound (Complex)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6ukj
Q-score: 0.505
Kim J, Tan YZ, Wicht KJ, Erramilli SK, Dhingra SK, Okombo J, Vendome J, Hagenah LM, Giacometti SI, Warren AL, Nosol K, Roepe PD, Potter CS, Carragher B, Kossiakoff AA, Quick M, Fidock DA, Mancia F
Nature (2019) 576 pp. 315-320 [ DOI: doi:10.1038/s41586-019-1795-x Pubmed: 31776516 ]
- Antigen-binding fragment (fab) (50 kDa, Cellular component from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Plasmodium falciparum chloroquine resistance transporter (pfcrt) 7g8 isoform complexed with fab (102 kDa, Cellular component)
- Plasmodium falciparum chloroquine resistance transporter (pfcrt) (49 kDa, Cellular component from Plasmodium falciparum 7G8)
- Fab heavy chain (25 kDa, Protein from Homo sapiens)
- Fab light chain (23 kDa, Protein from Homo sapiens)
- Chloroquine resistance transporter (53 kDa, Protein from Plasmodium falciparum (isolate 7G8))