CryoEM structure of human 20S proteasome in complex with proteasome inhibitor Salinosporamid A
Sulzen H, Fajtova P, O'Donoghue AJ, Boura E, Silhan J
bioRxiv (2025) [ DOI: doi:10.1101/2025.01.28.635221 Pubmed: 39974992 ]
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-2 (22 kDa, Protein from Homo sapiens)
- Human 20s proteasome in complex with proteasome inhibitor salinosporamid a (Complex from Homo sapiens)
- Proteasome subunit beta type-7 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- (3ar,6r,6as)-6-((s)-((s)-cyclohex-2-enyl)(hydroxy)methyl)-6a-methyl-4-oxo-hexahydro-2h-furo[3,2-c]pyrrole-6-carbaldehyde (279 Da, Ligand)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-5 (22 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-3 (22 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-1 (23 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-4 (24 kDa, Protein from Homo sapiens)
- Proteasome subunit beta type-6 (21 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
CryoEM structure of 20S Trichomonas vaginalis proteasome in complex with proteasome inhibitor CP-17
Resolution: 2.65 Å
EM Method: Single-particle
Fitted PDBs: 8p0t
Q-score: 0.617
Silhan J, Fajtova P, Bartosova J, Hurysz BM, Almaliti J, Miyamoto Y, Eckmann L, Gerwick WH, O'Donoghue AJ, Boura E
Nat Commun (2024) 15 pp. 8621-8621 [ Pubmed: 39366995 DOI: doi:10.1038/s41467-024-53022-w ]
- Family t1, proteasome alpha subunit, threonine peptidase (27 kDa, Protein from Trichomonas vaginalis G3)
- Family t1, proteasome alpha subunit, threonine peptidase (26 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit alpha type (26 kDa, Protein from Trichomonas vaginalis G3)
- N-[(2s)-1-[[(2s)-1-[[(1s)-1-[(2s,3r,5s,6r)-3-(hydroxymethyl)-5-methanoyl-2,3,6-trimethyl-morpholin-2-yl]-2-phenyl-ethyl]amino]-3-(1h-indol-3-yl)-1-oxidanylidene-propan-2-yl]amino]-3-(1h-indol-3-yl)-1-oxidanylidene-propan-2-yl]hexanamide (776 Da, Ligand)
- Family t1, proteasome alpha subunit, threonine peptidase (25 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (21 kDa, Protein from Trichomonas vaginalis G3)
- Ternary complex of 20s proteasome from trichomonas vaginalis (704 kDa, Complex from Trichomonas vaginalis G3)
- Proteasome subunit alpha type (27 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (23 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (22 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (21 kDa, Protein from Trichomonas vaginalis G3)
- Family t1, proteasome alpha subunit, threonine peptidase (25 kDa, Protein from Trichomonas vaginalis G3)
- Family t1, proteasome beta subunit, threonine peptidase (26 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome endopeptidase complex (26 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (25 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit alpha type (27 kDa, Protein from Trichomonas vaginalis G3)
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8oix
Q-score: 0.581
Silhan J, Fajtova P, Bartosova J, Hurysz BM, Almaliti J, Miyamoto Y, Eckmann L, Gerwick WH, O'Donoghue AJ, Boura E
Nat Commun (2024) 15 pp. 8621-8621 [ Pubmed: 39366995 DOI: doi:10.1038/s41467-024-53022-w ]
- Family t1, proteasome alpha subunit, threonine peptidase (27 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome endopeptidase complex (26 kDa, Protein from Trichomonas vaginalis G3)
- Family t1, proteasome alpha subunit, threonine peptidase (26 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit alpha type (26 kDa, Protein from Trichomonas vaginalis G3)
- Family t1, proteasome alpha subunit, threonine peptidase (25 kDa, Protein from Trichomonas vaginalis G3)
- (3ar,6r,6as)-6-((s)-((s)-cyclohex-2-enyl)(hydroxy)methyl)-6a-methyl-4-oxo-hexahydro-2h-furo[3,2-c]pyrrole-6-carbaldehyde (279 Da, Ligand)
- Proteasome subunit beta (21 kDa, Protein from Trichomonas vaginalis G3)
- Ternary complex of 20s proteasome from trichomonas vaginalis (704 kDa, Complex from Trichomonas vaginalis G3)
- Proteasome subunit alpha type (27 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (23 kDa, Protein from Trichomonas vaginalis G3)
- Family t1, proteasome beta subunit, threonine peptidase (26 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (21 kDa, Protein from Trichomonas vaginalis G3)
- Family t1, proteasome alpha subunit, threonine peptidase (25 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (25 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit alpha type (27 kDa, Protein from Trichomonas vaginalis G3)
- Proteasome subunit beta (22 kDa, Protein from Trichomonas vaginalis G3)
Structure and function based design of Plasmodium-selective proteasome inhibitors
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5fmg
Q-score: 0.47
Li H, O' Donoghue AJ, van der Linden WA, Xie SC, Yoo E, Foe IT, Tilley L, Craik CS, da Fonseca PCA, Bogyo M
Nature (2016) 530 pp. 233-236 [ DOI: doi:10.1038/nature16936 Pubmed: 26863983 ]