Partial auto-inhibitory complex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8g99
Q-score: 0.551
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Polymerase alpha (200 kDa, Complex from Xenopus laevis)
- Polymerase alpha-primase (110 kDa, Complex)
- Iron/sulfur cluster (351 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
Complete auto-inhibitory complex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8g9f
Q-score: 0.48
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Polymerase alpha (200 kDa, Complex from Xenopus laevis)
- Polymerase alpha-primase (110 kDa, Complex)
- Dna primase (49 kDa, Protein from Xenopus laevis)
- Zinc ion (65 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha subunit b (67 kDa, Protein from Xenopus laevis)
DNA initiation subcomplex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8g9l
Q-score: 0.453
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Dna template (15 kDa, DNA from synthetic construct)
- Rna primer (2 kDa, RNA from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Polymerase alpha (Complex from Xenopus laevis)
- Polymerase alpha-primase with a dna initiation substrate (110 kDa, Complex)
- Dna initiation substrate (20 kDa, Complex from synthetic construct)
Partial DNA elongation subcomplex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8g9n
Q-score: 0.415
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Dna template (15 kDa, DNA from synthetic construct)
- Rna-dna primer (6 kDa, DNA from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Polymerase alpha (Complex from Xenopus laevis)
- Polymerase alpha-primase with a dna elongation substrate (20 kDa, Complex)
- Dna elongation substrate (Complex from synthetic construct)
Complete DNA elongation subcomplex of Xenopus laevis DNA polymerase alpha-primase
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8g9o
Q-score: 0.275
Mullins EA, Salay LE, Durie CL, Bradley NP, Jackman JE, Ohi MD, Chazin WJ, Eichman BF
Nat Struct Mol Biol (2024) 31 pp. 777-790 [ Pubmed: 38491139 DOI: doi:10.1038/s41594-024-01227-4 ]
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Xenopus laevis)
- Primase (Complex from Xenopus laevis)
- Dna primase large subunit (59 kDa, Protein from Xenopus laevis)
- Magnesium ion (24 Da, Ligand)
- Dna template (15 kDa, DNA from synthetic construct)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Polymerase alpha (Complex from Xenopus laevis)
- Polymerase alpha-primase with a dna elongation substrate (110 kDa, Complex)
- Dna elongation substrate (20 kDa, Complex from synthetic construct)
- Rna-dna primer (6 kDa, Macromolecule from synthetic construct)
CryoEM structure of the Caveolin-1 8S complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7sc0
Q-score: 0.502
Porta JC, Han B, Gulsevin A, Chung JM, Peskova Y, Connolly S, Mchaourab HS, Meiler J, Karakas E, Kenworthy AK, Ohi MD
Sci Adv (2022) 8 pp. eabn7232-eabn7232 [ Pubmed: 35544577 DOI: doi:10.1126/sciadv.abn7232 ]
- Caveolin-1 8s complex with c11 symmetry. (Complex from Homo sapiens)
- Caveolin-1 (20 kDa, Protein from Homo sapiens)
Legionella pneumophila Dot/Icm T4SS C1 Reconstruction
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7muc
Q-score: 0.393
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss c1 (Complex from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)
Legionella pneumophila Dot/Icm T4SS OMC
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mud
Q-score: 0.564
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss omc (Complex from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)
Legionella pneumophila Dot/Icm T4SS PR
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mue
Q-score: 0.601
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss pr (Complex from Legionella pneumophila)
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 1
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7muq
Q-score: 0.335
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss c1 (Complex from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)