Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8urp
Q-score: 0.589
Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S
Nat Commun (2025) 16 pp. 111-111 [ Pubmed: 39747155 DOI: doi:10.1038/s41467-024-55673-1 ]
- Nanobody25 (Complex from Lama glama)
- Cholinephosphotransferase 1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- [2-cytidylate-o'-phosphonyloxyl]-ethyl-trimethyl-ammonium (488 Da, Ligand)
- Ycpt1 (Complex from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phoshocholine (734 Da, Ligand)
Cholinephosphotransferase in complex with selective inhibitor chelerythrine
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8urt
Q-score: 0.546
Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S
Nat Commun (2025) 16 pp. 111-111 [ Pubmed: 39747155 DOI: doi:10.1038/s41467-024-55673-1 ]
- Nanobody25 (Complex from Lama glama)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Ycpt1 (Complex from Saccharomyces cerevisiae)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- 1,2-dimethoxy-12-methyl[1,3]benzodioxolo[5,6-c]phenanthridin-12-ium (348 Da, Ligand)
- Cholinephosphotransferase 1 (44 kDa, Protein from Saccharomyces cerevisiae)
Cholinephosphotransferase in complex with diacylglycerol
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8ul9
Q-score: 0.503
Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S
Nat Commun (2025) 16 pp. 111-111 [ Pubmed: 39747155 DOI: doi:10.1038/s41467-024-55673-1 ]
- Nanobody24 (Complex from Lama glama)
- Cholinephosphotransferase 1 (44 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Ycpt1 (Complex from Saccharomyces cerevisiae)
- Diacyl glycerol (625 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Saccharomyces cerevisiae cdp-choline phosphotransferase in complex with diacyl-glycerol (Complex from Saccharomyces cerevisiae)
Legionella pneumophila Dot/Icm T4SS C1 Reconstruction
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7muc
Q-score: 0.393
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss c1 (Complex from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)
Legionella pneumophila Dot/Icm T4SS OMC
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mud
Q-score: 0.564
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss omc (Complex from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)
Legionella pneumophila Dot/Icm T4SS PR
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mue
Q-score: 0.601
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss pr (Complex from Legionella pneumophila)
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 1
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7muq
Q-score: 0.335
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss c1 (Complex from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 2
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7mus
Q-score: 0.305
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Reconstruction of the legionella pneumophila dot/icm t4ss 3dva map 2 (Complex from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 3
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7muv
Q-score: 0.316
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss 3dva map 3 (Complex from Legionella pneumophila)
Reconstruction of the Legionella pneumophila Dot/Icm T4SS 3DVA Map 4
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7muw
Q-score: 0.282
Sheedlo MJ, Durie CL, Chung JM, Chang L, Roberts J, Swanson M, Lacy DB, Ohi MD
eLife (2021) 10 [ Pubmed: 34519271 DOI: doi:10.7554/eLife.70427 ]
- Type iv secretion protein icmk (38 kDa, Protein from Legionella pneumophila)
- Neurogenic locus notch (13 kDa, Protein from Legionella pneumophila)
- Legionella pneumophila dot/icm t4ss 3dva map 4 (Complex from Legionella pneumophila)
- Dotd (17 kDa, Protein from Legionella pneumophila)
- Outer membrane protein, ompa family protein (27 kDa, Protein from Legionella pneumophila)
- Inner membrane lipoprotein yiad (21 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (4 kDa, Protein from Legionella pneumophila)
- Icme protein (107 kDa, Protein from Legionella pneumophila)
- Duf2807 domain-containing protein (36 kDa, Protein from Legionella pneumophila)
- Dotf (29 kDa, Protein from Legionella pneumophila)
- Unknown protein fragment (783 Da, Protein from Legionella pneumophila)
- Dotc (34 kDa, Protein from Legionella pneumophila)