Cryo-EM structure of the full-length human P2X4 receptor in the apo closed state
Shi H, Ditter IA, Oken AC, Mansoor SE
Sci Adv (2025) 11 pp. eadr3315-eadr3315 [ Pubmed: 39823330 DOI: doi:10.1126/sciadv.adr3315 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- P2x purinoceptor 4 (43 kDa, Protein from Homo sapiens)
- Membrane protein (Complex from Homo sapiens)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM structure of BAY-1797 bound to the full-length human P2X4 receptor in the closed state
Shi H, Ditter IA, Oken AC, Mansoor SE
Sci Adv (2025) 11 pp. eadr3315-eadr3315 [ Pubmed: 39823330 DOI: doi:10.1126/sciadv.adr3315 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- P2x purinoceptor 4 (43 kDa, Protein from Homo sapiens)
- N-[4-(3-chloranylphenoxy)-3-sulfamoyl-phenyl]-2-phenyl-ethanamide (416 Da, Ligand)
- Membrane protein (Complex from Homo sapiens)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM structure of the full-length human P2X4 receptor in the ATP-bound desensitized state
Shi H, Ditter IA, Oken AC, Mansoor SE
Sci Adv (2025) 11 pp. eadr3315-eadr3315 [ Pubmed: 39823330 DOI: doi:10.1126/sciadv.adr3315 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- P2x purinoceptor 4 (43 kDa, Protein from Homo sapiens)
- Membrane protein (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM structure of the rat P2X7 receptor in complex with the allosteric antagonist A438079
Resolution: 2.18 Å
EM Method: Single-particle
Fitted PDBs: 8tr6
Q-score: 0.669
Oken AC, Ditter IA, Lisi NE, Krishnamurthy I, Godsey MH, Mansoor SE
Sci Adv (2024) 10 pp. eado5084-eado5084 [ DOI: doi:10.1126/sciadv.ado5084 Pubmed: 39365862 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 3-{[(5r)-5-(2,3-dichlorophenyl)tetrazolidin-1-yl]methyl}pyridine (310 Da, Ligand)
- Palmitic acid (256 Da, Ligand from Rattus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- P2x purinoceptor 7 (68 kDa, Protein from Rattus)
- Sodium ion (22 Da, Ligand)
- Membrane protein (Complex from Rattus)
- Water (18 Da, Ligand)
Cryo-EM structure of the rat P2X7 receptor in complex with the allosteric antagonist A839977
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8tr7
Q-score: 0.619
Oken AC, Ditter IA, Lisi NE, Krishnamurthy I, Godsey MH, Mansoor SE
Sci Adv (2024) 10 pp. eado5084-eado5084 [ DOI: doi:10.1126/sciadv.ado5084 Pubmed: 39365862 ]
- (1p)-1-(2,3-dichlorophenyl)-n-({2-[(pyridin-2-yl)oxy]phenyl}methyl)-1h-tetrazol-5-amine (413 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium ion (22 Da, Ligand from Rattus)
- Palmitic acid (256 Da, Ligand from Rattus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- P2x purinoceptor 7 (68 kDa, Protein from Rattus)
- Membrane protein (Complex from Rattus)
- Water (18 Da, Ligand)
Cryo-EM structure of the rat P2X7 receptor in complex with the allosteric antagonist AZD9056
Resolution: 2.21 Å
EM Method: Single-particle
Fitted PDBs: 8tr8
Q-score: 0.66
Oken AC, Ditter IA, Lisi NE, Krishnamurthy I, Godsey MH, Mansoor SE
Sci Adv (2024) 10 pp. eado5084-eado5084 [ DOI: doi:10.1126/sciadv.ado5084 Pubmed: 39365862 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- N-{[(3s,5s,7s)-adamantan-1-yl]methyl}-2-chloro-5-{3-[(3-hydroxypropyl)amino]propyl}benzamide (419 Da, Ligand)
- Palmitic acid (256 Da, Ligand from Rattus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- P2x purinoceptor 7 (68 kDa, Protein from Rattus)
- Sodium ion (22 Da, Ligand)
- Membrane protein (Complex from Rattus)
- Water (18 Da, Ligand)
Cryo-EM structure of the rat P2X7 receptor in complex with the allosteric antagonist GSK1482160
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 8tra
Q-score: 0.632
Oken AC, Ditter IA, Lisi NE, Krishnamurthy I, Godsey MH, Mansoor SE
Sci Adv (2024) 10 pp. eado5084-eado5084 [ DOI: doi:10.1126/sciadv.ado5084 Pubmed: 39365862 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand from Rattus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- P2x purinoceptor 7 (68 kDa, Protein from Rattus)
- Sodium ion (22 Da, Ligand)
- N-{[2-chloro-3-(trifluoromethyl)phenyl]methyl}-1-methyl-5-oxo-l-prolinamide (334 Da, Ligand)
- Membrane protein (Complex from Rattus)
- Water (18 Da, Ligand)
Cryo-EM structure of the rat P2X7 receptor in complex with the allosteric antagonist JNJ47965567
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 8trb
Q-score: 0.641
Oken AC, Ditter IA, Lisi NE, Krishnamurthy I, Godsey MH, Mansoor SE
Sci Adv (2024) 10 pp. eado5084-eado5084 [ DOI: doi:10.1126/sciadv.ado5084 Pubmed: 39365862 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand from Rattus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- P2x purinoceptor 7 (68 kDa, Protein from Rattus)
- Sodium ion (22 Da, Ligand)
- N-{[4-(4-phenylpiperazin-1-yl)oxan-4-yl]methyl}-2-(phenylsulfanyl)pyridine-3-carboxamide (488 Da, Ligand)
- Membrane protein (Complex from Rattus)
- Water (18 Da, Ligand)
Cryo-EM structure of the rat P2X7 receptor in complex with the allosteric antagonist methyl blue
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8trk
Q-score: 0.573
Oken AC, Ditter IA, Lisi NE, Krishnamurthy I, Godsey MH, Mansoor SE
Sci Adv (2024) 10 pp. eado5084-eado5084 [ DOI: doi:10.1126/sciadv.ado5084 Pubmed: 39365862 ]
- Zinc ion (65 Da, Ligand)
- 4,4',4''-[methanetriyltris(4,1-phenyleneazanediyl)]tri(benzene-1-sulfonic acid) (757 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Palmitic acid (256 Da, Ligand from Rattus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- P2x purinoceptor 7 (68 kDa, Protein from Rattus)
- Membrane protein (Complex from Rattus)
- Water (18 Da, Ligand)
Cryo-EM structure of the human P2X1 receptor in the apo closed state
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 9c2a
Q-score: 0.536
Oken AC, Lisi NE, Ditter IA, Shi H, Nechiporuk NA, Mansoor SE
Nat Commun (2024) 15 pp. 8490-8490 [ Pubmed: 39353889 DOI: doi:10.1038/s41467-024-52636-4 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- P2x purinoceptor 1 (45 kDa, Protein from Homo sapiens)
- Membrane protein (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Water (18 Da, Ligand)