Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate
Kieuvongngam V, Olinares PDB, Palillo A, Oldham ML, Chait BT, Chen J
eLife (2020) 9 [ DOI: 10.7554/elife.51492 Pubmed: 31934861 ]
Image sets:
Cryo-EM structure of TACAN in the apo form (TMEM120A)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7n0k
Q-score: 0.43
Niu Y, Tao X, Vaisey G, Olinares PDB, Alwaseem H, Chait BT, MacKinnon R
eLife (2021) 10 [ Pubmed: 34374644 DOI: doi:10.7554/eLife.71188 ]
- Ion channel tacan (40 kDa, Protein from Mus musculus)
- Homo-dimeric assembly of wild type tacan(tmem120a) (Complex from Mus musculus)
Cryo-EM structure of TACAN in the H196A H197A mutant form (TMEM120A)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7n0l
Q-score: 0.573
Niu Y, Tao X, Vaisey G, Olinares PDB, Alwaseem H, Chait BT, MacKinnon R
eLife (2021) 10 [ Pubmed: 34374644 DOI: doi:10.7554/eLife.71188 ]
- Coenzyme a (767 Da, Ligand)
- Ion channel tacan (40 kDa, Protein from Mus musculus)
- Homo-dimeric assembly of h196a h197a mutant tacan(tmem120a) (Complex from Mus musculus)
Structure of SARS-CoV-2 backtracked complex bound to nsp13 helicase - nsp13(1)-BTC
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7krn
Q-score: 0.476
Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA
PNAS (2021) 118 [ Pubmed: 33883267 DOI: doi:10.1073/pnas.2102516118 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Sars-cov-2 backtracked complex bound to nsp13 helicase - nsp13(1)-btc (Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (37-mer) (12 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Chapso (631 Da, Ligand)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (43-mer) (17 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Structure of SARS-CoV-2 backtracked complex complex bound to nsp13 helicase - nsp13(2)-BTC
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7kro
Q-score: 0.416
Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA
PNAS (2021) 118 [ Pubmed: 33883267 DOI: doi:10.1073/pnas.2102516118 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aluminum fluoride (83 Da, Ligand)
- Helicase (67 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 backtracked complex complex bound to nsp13 helicase - nsp13(2)-btc (Complex from Severe acute respiratory syndrome coronavirus 2)
- Rna (37-mer) (12 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Chapso (631 Da, Ligand)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (43-mer) (17 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Structure of SARS-CoV-2 backtracked complex complex bound to nsp13 helicase - BTC (local refinement)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7krp
Q-score: 0.361
Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA
PNAS (2021) 118 [ Pubmed: 33883267 DOI: doi:10.1073/pnas.2102516118 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 backtracked complex complex bound to nsp13 helicase - btc (local refinement) (Complex from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (37-mer) (12 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Rna (36-mer) (17 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Chapso (631 Da, Ligand)
- Non-structural protein 8 (22 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Mfd-bound E.coli RNA polymerase elongation complex - L1 state
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6x26
Q-score: 0.339
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Escherichia coli mfd-rnap elonation complex: state l1 (570 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - L2 state
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6x2f
Q-score: 0.341
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Escherichia coli mfd-rnap elongation complex: l2 state (570 kDa, Complex from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - I state
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6x2n
Q-score: 0.368
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Escherichia coli mfd-rna polymerase elongation complex: i state (570 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - II state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6x43
Q-score: 0.476
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Escherichia coli mfd-rna polymerase elongation complex: state ii (Complex from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)