Mfd-bound E.coli RNA polymerase elongation complex - I state
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6x2n
Q-score: 0.368
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Escherichia coli mfd-rna polymerase elongation complex: i state (570 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - II state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6x43
Q-score: 0.476
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Escherichia coli mfd-rna polymerase elongation complex: state ii (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - III state
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6x4w
Q-score: 0.375
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Escherichia coli mfd-rna polymerase elongation complex: state iii (570 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - IV state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6x4y
Q-score: 0.443
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Escherichia coli mfd-rna polymerase elongation complex: state iv (570 kDa, Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - V state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6x50
Q-score: 0.494
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ DOI: doi:10.7554/eLife.62117 Pubmed: 33480355 ]
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Escherichia coli mfd-rna polymerase elongation complex: state v (570 kDa, Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
Escherichia coli RNA polymerase closed complex (TRPc) with TraR and rpsT P2 promoter
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6psq
Q-score: 0.479
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA
Mol Cell (2020) 78 pp. 275- [ DOI: doi:10.1016/j.molcel.2020.02.017 Pubmed: 32160514 ]
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Escherichia coli sigma70-holoenzyme bound to trar and wildtype rpst p2 as trpc (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6psr
Q-score: 0.48
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA
Mol Cell (2020) 78 pp. 275- [ DOI: doi:10.1016/j.molcel.2020.02.017 Pubmed: 32160514 ]
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Escherichia coli sigma70-holoenzyme bound to trar and wildtype rpst p2 as trpi1 (Complex from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6pss
Q-score: 0.446
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA
Mol Cell (2020) 78 pp. 275- [ DOI: doi:10.1016/j.molcel.2020.02.017 Pubmed: 32160514 ]
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Escherichia coli sigma70-holoenzyme bound to trar and mutant rpst p2 as trpi1.5a (Complex from Escherichia coli)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6pst
Q-score: 0.514
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA
Mol Cell (2020) 78 pp. 275- [ DOI: doi:10.1016/j.molcel.2020.02.017 Pubmed: 32160514 ]
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Escherichia coli sigma70-holoenzyme bound to trar and mutant rpst p2 as trpi1.5b (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Protein trar (8 kDa, Protein from Escherichia coli)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6psu
Q-score: 0.426
Chen J, Chiu C, Gopalkrishnan S, Chen AY, Olinares PDB, Saecker RM, Winkelman JT, Maloney MF, Chait BT, Ross W, Gourse RL, Campbell EA, Darst SA
Mol Cell (2020) 78 pp. 275- [ DOI: doi:10.1016/j.molcel.2020.02.017 Pubmed: 32160514 ]
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Escherichia coli sigma70-holoenzyme bound to trar and wildtype rpst p2 as trpi2 (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Dna (85-mer) (26 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)