Cryo-EM structure of the yeast SPT-Orm2-Dimer complex, local refinement of a monomer
Korner C, Schafer JH, Esch BM, Parey K, Walter S, Teis D, Januliene D, Schmidt O, Moeller A, Frohlich F
Cell Rep (2024) 43 pp. 114627-114627 [ DOI: doi:10.1016/j.celrep.2024.114627 Pubmed: 39167489 ]
- Complex of lcb1, lcb2, orm2 and tsc3 (160 kDa, Complex from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast SPT-Orm2-Dimer complex
Korner C, Schafer JH, Esch BM, Parey K, Walter S, Teis D, Januliene D, Schmidt O, Moeller A, Frohlich F
Cell Rep (2024) 43 pp. 114627-114627 [ DOI: doi:10.1016/j.celrep.2024.114627 Pubmed: 39167489 ]
- Orm2 isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- C2 symmetric complex of lcb1, lcb2, orm2 and tsc3 (320 kDa, Complex from Saccharomyces cerevisiae)
- ~{n}-[(2~{s},3~{s},4~{r})-1,3,4-tris(oxidanyl)octadecan-2-yl]heptacosanamide (710 Da, Ligand)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Serine palmitoyltransferase 1 (64 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast SPT-Orm2-Monomer complex
Korner C, Schafer JH, Esch BM, Parey K, Walter S, Teis D, Januliene D, Schmidt O, Moeller A, Frohlich F
Cell Rep (2024) 43 pp. 114627-114627 [ DOI: doi:10.1016/j.celrep.2024.114627 Pubmed: 39167489 ]
- Orm2 isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- ~{n}-[(2~{s},3~{s},4~{r})-1,3,4-tris(oxidanyl)octadecan-2-yl]heptacosanamide (710 Da, Ligand)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- C2 symmetric complex of lcb1, lcb2, orm2 and tsc3 (160 kDa, Complex from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 1 (64 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
ABCB1 L335C mutant (mABCB1) in the inward facing state bound to AAC
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8pee
Q-score: 0.379
Gewering T, Waghray D, Parey K, Jung H, Tran NNB, Zapata J, Zhao P, Chen H, Januliene D, Hummer G, Urbatsch I, Moeller A, Zhang Q
eLife (2024) 12 [ DOI: doi:10.7554/eLife.90174 Pubmed: 38259172 ]
- (4s,11s,18s)-4-[[(2,4-dinitrophenyl)disulfanyl]methyl]-11,18-dimethyl-6,13,20-trithia-3,10,17,22,23,24-hexazatetracyclo[17.2.1.1^{5,8}.1^{12,15}]tetracosa-1(21),5(24),7,12(23),14,19(22)-hexaene-2,9,16-trione (692 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Atp-dependent translocase abcb1 (146 kDa, Protein from Mus musculus)
- Abcb1 (140 MDa, Complex from Mus musculus)
- (4~{s},11~{s},18~{s})-4,11-dimethyl-18-(sulfanylmethyl)-6,13,20-trithia-3,10,17,22,23,24-hexazatetracyclo[17.2.1.1^{5,8}.1^{12,15}]tetracosa-1(21),5(24),7,12(23),14,19(22)-hexaene-2,9,16-trione (494 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8pok
Q-score: 0.501
Kock Z, Schnelle K, Persechino M, Umbach S, Schihada H, Januliene D, Parey K, Pockes S, Kolb P, Dotsch V, Moller A, Hilger D, Bernhard F
Nat Commun (2024) 15 pp. 1831-1831 [ DOI: doi:10.1038/s41467-024-46096-z Pubmed: 38418462 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Histamine h2 receptor (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Histamine (111 Da, Ligand)
- H2 receptor coupled to heterotrimeric gs protein (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 and guanine nucleotide-binding protein subunit gamma (fragment) (Complex from Homo sapiens)
- Histamine h2 receptor (41 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
Cryo-EM structure of the yeast SPT-Orm1-Monomer complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8c80
Q-score: 0.463
Schafer JH, Korner C, Esch BM, Limar S, Parey K, Walter S, Januliene D, Moeller A, Frohlich F
Nat Commun (2023) 14 pp. 6196-6196 [ DOI: doi:10.1038/s41467-023-41747-z Pubmed: 37794019 ]
- Ergosterol (396 Da, Ligand)
- Protein orm1 (25 kDa, Protein from Saccharomyces cerevisiae)
- N-[(2s,3s,4r)-1,3,4-trihydroxyoctadecan-2-yl]hexacosanamide (696 Da, Ligand)
- Heterotetrameric complex of orm1 with lcb1, lcb2 and tsc3 (160 kDa, Complex from Saccharomyces cerevisiae)
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Serine palmitoyltransferase 1 (64 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast SPT-Orm1-Sac1 complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8c81
Q-score: 0.525
Schafer JH, Korner C, Esch BM, Limar S, Parey K, Walter S, Januliene D, Moeller A, Frohlich F
Nat Commun (2023) 14 pp. 6196-6196 [ DOI: doi:10.1038/s41467-023-41747-z Pubmed: 37794019 ]
- Ergosterol (396 Da, Ligand)
- Protein orm1 (25 kDa, Protein from Saccharomyces cerevisiae)
- N-[(2s,3s,4r)-1,3,4-trihydroxyoctadecan-2-yl]hexacosanamide (696 Da, Ligand)
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Phosphatidylinositol-3-phosphatase sac1 (71 kDa, Protein from Saccharomyces cerevisiae)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Heteropentameric complex of orm1 with lcb1, lcb2, tsc3 and sac1 (230 kDa, Complex from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 1 (64 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast SPT-Orm1-Dimer complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8c82
Q-score: 0.488
Schafer JH, Korner C, Esch BM, Limar S, Parey K, Walter S, Januliene D, Moeller A, Frohlich F
Nat Commun (2023) 14 pp. 6196-6196 [ DOI: doi:10.1038/s41467-023-41747-z Pubmed: 37794019 ]
- Serine palmitoyltransferase 1 (62 kDa, Protein from Saccharomyces cerevisiae)
- N-[(2s,3s,4r)-1,3,4-trihydroxyoctadecan-2-yl]hexacosanamide (696 Da, Ligand)
- Protein orm1 (25 kDa, Protein from Saccharomyces cerevisiae)
- Serine palmitoyltransferase-regulating protein tsc3 (9 kDa, Protein from Saccharomyces cerevisiae)
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Complex of orm1 with lcb1, lcb2 and tsc3 (325 kDa, Complex from Saccharomyces cerevisiae)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Serine palmitoyltransferase 2 (63 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast SPT-Orm1-Dimer complex, local refinement of a monomer
Schafer JH, Korner C, Esch BM, Limar S, Parey K, Walter S, Januliene D, Moeller A, Frohlich F
Nat Commun (2023) 14 pp. 6196-6196 [ DOI: doi:10.1038/s41467-023-41747-z Pubmed: 37794019 ]
The ABCB1 L335C mutant (mABCB1) in the Apo state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8avy
Q-score: 0.54
Gewering T, Waghray D, Parey K, Jung H, Tran NNB, Zapata J, Zhao P, Chen H, Januliene D, Hummer G, Urbatsch I, Moeller A, Zhang Q
eLife (2024) 12 [ DOI: doi:10.7554/eLife.90174 Pubmed: 38259172 ]
- Water (18 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Atp-dependent translocase abcb1 (146 kDa, Protein from Mus musculus)
- Abcb1 (140 MDa, Complex from Mus musculus)