Nanodisc reconstituted MsbA in complex with nanobodies, spin-labeled at position A60C
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7ph2
Q-score: 0.394
Galazzo L, Meier G, Januliene D, Parey K, De Vecchis D, Striednig B, Hilbi H, Schafer LV, Kuprov I, Moeller A, Bordignon E, Seeger MA
Sci Adv (2022) 8 pp. eabn6845-eabn6845 [ Pubmed: 36223470 DOI: doi:10.1126/sciadv.abn6845 ]
- (1~{r},4~{r},11~{s},14~{s},19~{z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione (524 Da, Ligand)
- Atp-dependent lipid a-core flippase (65 kDa, Protein from Escherichia coli K-12)
- E. coli msba in complex with nanobody nb_msba#1, labeled with gd-dota at cys60 (Complex from Escherichia coli)
- Gadolinium atom (157 Da, Ligand)
- Nb_msba#1 (12 kDa, Protein from Vicugna pacos)
- (2~{r},4~{r},5~{r},6~{r})-6-[(1~{r})-1,2-bis(oxidanyl)ethyl]-4-[(2~{r},3~{s},4~{s},5~{r},6~{r})-6-[(1~{s})-1,2-bis(oxidanyl)ethyl]-4-[(2~{r},3~{s},4~{s},5~{s},6~{r})-6-[(1~{s})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-2-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-4-[(3~{r})-3-nonanoyloxytetradecanoyl]oxy-5-[[(3~{r})-3-octanoyloxytetradecanoyl]amino]-6-[[(2~{r},3~{s},4~{s},5~{s},6~{r})-3-oxidanyl-5-[[(3~{r})-3-oxidanylnonanoyl]amino]-4-[(3~{r})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-oxan-2-yl]methoxy]-5-oxidanyl-oxane-2-carboxylic acid (2 kDa, Ligand)
AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position A60C
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ph3
Q-score: 0.555
Galazzo L, Meier G, Januliene D, Parey K, De Vecchis D, Striednig B, Hilbi H, Schafer LV, Kuprov I, Moeller A, Bordignon E, Seeger MA
Sci Adv (2022) 8 pp. eabn6845-eabn6845 [ Pubmed: 36223470 DOI: doi:10.1126/sciadv.abn6845 ]
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Atp-dependent lipid a-core flippase (65 kDa, Protein from Escherichia coli K-12)
- (1~{r},4~{r},11~{s},14~{s},19~{z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione (524 Da, Ligand)
- Water (18 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- E. coli msba in complex with nanobody nb_msba#1, labeled with gd-dota at cys60 (Complex from Escherichia coli)
- Gadolinium atom (157 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Nanobody nb_msba#1 (12 kDa, Protein from Vicugna pacos)
AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position T68C
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ph4
Q-score: 0.569
Galazzo L, Meier G, Januliene D, Parey K, De Vecchis D, Striednig B, Hilbi H, Schafer LV, Kuprov I, Moeller A, Bordignon E, Seeger MA
Sci Adv (2022) 8 pp. eabn6845-eabn6845 [ Pubmed: 36223470 DOI: doi:10.1126/sciadv.abn6845 ]
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- (1~{r},4~{r},11~{s},14~{s},19~{z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione (524 Da, Ligand)
- Nanobody nb_msba#1 (12 kDa, Protein from Vicugna pacos)
- Water (18 Da, Ligand)
- Atp-dependent lipid a-core flippase (65 kDa, Protein from Escherichia coli (strain K12))
- Gadolinium atom (157 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Amp-pnp bound e. coli msba in complex with nanobody nb_msba#1, labeled with gd-dota at t68c (Complex)
Nanodisc reconstituted MsbA in complex with nanobodies, spin-labeled at position T68C
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7ph7
Q-score: 0.419
Galazzo L, Meier G, Januliene D, Parey K, De Vecchis D, Striednig B, Hilbi H, Schafer LV, Kuprov I, Moeller A, Bordignon E, Seeger MA
Sci Adv (2022) 8 pp. eabn6845-eabn6845 [ Pubmed: 36223470 DOI: doi:10.1126/sciadv.abn6845 ]
- E. coli msba in complex with nanobody nb_msba#1, labeled with gd-dota at t68c (Complex from Escherichia coli)
- (1~{r},4~{r},11~{s},14~{s},19~{z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione (524 Da, Ligand)
- Gadolinium atom (157 Da, Ligand)
- Atp-binding transport protein multicopy suppressor of htrb (65 kDa, Protein from Escherichia coli)
- (2~{r},4~{r},5~{r},6~{r})-6-[(1~{r})-1,2-bis(oxidanyl)ethyl]-4-[(2~{r},3~{s},4~{s},5~{r},6~{r})-6-[(1~{s})-1,2-bis(oxidanyl)ethyl]-4-[(2~{r},3~{s},4~{s},5~{s},6~{r})-6-[(1~{s})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-3,5-bis(oxidanyl)oxan-2-yl]oxy-2-[[(2~{r},3~{s},4~{r},5~{r},6~{r})-4-[(3~{r})-3-nonanoyloxytetradecanoyl]oxy-5-[[(3~{r})-3-octanoyloxytetradecanoyl]amino]-6-[[(2~{r},3~{s},4~{s},5~{s},6~{r})-3-oxidanyl-5-[[(3~{r})-3-oxidanylnonanoyl]amino]-4-[(3~{r})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-oxan-2-yl]methoxy]-5-oxidanyl-oxane-2-carboxylic acid (2 kDa, Ligand)
- Nb_msba#1 (12 kDa, Protein from Vicugna pacos)
Subtomogram average structure of anammoxosomal nitrite oxidoreductase
Chicano TM, Dietrich L, de Almeida NM, Akram M, Hartmann E, Leidreiter F, Leopoldus D, Mueller M, Sanchez R, Nuijten GHL, Reimann J, Seifert KA, Schlichting I, van Niftrik L, Jetten MSM, Dietl A, Kartal B, Parey K, Barends TRM
Nat Microbiol (2021) 6 pp. 1129-1139 [ Pubmed: 34267357 DOI: doi:10.1038/s41564-021-00934-8 ]
- Anammoxosomal tubules of nxr and nxr-t (Complex from Candidatus Kuenenia stuttgartiensis)
- Nitrite oxidoreductase subunit c (Protein from Candidatus Kuenenia stuttgartiensis)
- Nitrite oxidoreductase subunit a (Protein from Candidatus Kuenenia stuttgartiensis)
- Nitrite oxidoreductase subunit b (Protein from Candidatus Kuenenia stuttgartiensis)
- Conserved hypothetical (monoheme) protein (Protein from Candidatus Kuenenia stuttgartiensis)
Helical reconstruction of nitrite oxidoreductase from anammox bacteria
Chicano TM, Dietrich L, de Almeida NM, Akram M, Hartmann E, Leidreiter F, Leopoldus D, Mueller M, Sanchez R, Nuijten GHL, Reimann J, Seifert KA, Schlichting I, van Niftrik L, Jetten MSM, Dietl A, Kartal B, Parey K, Barends TRM
Nat Microbiol (2021) 6 pp. 1129-1139 [ Pubmed: 34267357 DOI: doi:10.1038/s41564-021-00934-8 ]
- Nitrite oxidoreductase subunit c (Protein from Candidatus Kuenenia stuttgartiensis)
- Nitrite oxidoreductase subunit a (Protein from Candidatus Kuenenia stuttgartiensis)
- Nitrite oxidoreductase (230 MDa, Complex from Candidatus Kuenenia stuttgartiensis)
- Nitrite oxidoreductase subunit b (Protein from Candidatus Kuenenia stuttgartiensis)
- Conserved hypothetical (monoheme) protein (Protein from Candidatus Kuenenia stuttgartiensis)
Cryo-EM structure of the hydrazine dehydrogenase from Kuenia stuttgartiensis in the octameric state
Akram M, Dietl A, Mersdorf U, Prinz S, Maalcke W, Keltjens J, Ferousi C, de Almeida NM, Reimann J, Kartal B, Jetten MSM, Parey K, Barends TRM
Sci Adv (2019) 5 pp. eaav4310-eaav4310 [ DOI: doi:10.1126/sciadv.aav4310 Pubmed: 31001586 ]
Akram M, Dietl A, Mersdorf U, Prinz S, Maalcke W, Keltjens J, Ferousi C, de Almeida NM, Reimann J, Kartal B, Jetten MSM, Parey K, Barends TRM
Sci Adv (2019) 5 pp. eaav4310-eaav4310 [ DOI: doi:10.1126/sciadv.aav4310 Pubmed: 31001586 ]
Cryo-EM structure of the hydrazine dehydrogenase from Kuenia stuttgartiensis in the decameric state
Akram M, Dietl A, Mersdorf U, Prinz S, Maalcke W, Keltjens J, Ferousi C, de Almeida NM, Reimann J, Kartal B, Jetten MSM, Parey K, Barends TRM
Sci Adv (2019) 5 pp. eaav4310-eaav4310 [ DOI: doi:10.1126/sciadv.aav4310 Pubmed: 31001586 ]