Xu X, Godoy-Ruiz R, Adipietro KA, Peralta C, Ben-Hail D, Varney KM, Cook ME, Roth BM, Wilder PT, Cleveland T, Grishaev A, Neu HM, Michel SLJ, Yu W, Beckett D, Rustandi RR, Lancaster C, Loughney JW, Kristopeit A, Christanti S, Olson JW, MacKerell AD, Georges AD, Pozharski E, Weber DJ
Proc Natl Acad Sci U S A (2020) 117 pp. 1049-1058 [ DOI: 10.1073/pnas.1919490117 Pubmed: 31896582 ]
Image sets:
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8g6u
Q-score: 0.433
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ DOI: doi:10.1038/s41467-023-42500-2 Pubmed: 37872202 ]
- Light chain of 10-1074 (23 kDa, Protein from Homo sapiens)
- Crf01_ae t/f100 hiv-1 gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Cryo-em structure of t/f100 sosip.664 hiv-1 env trimer with lmhs mutations in complex with 8anc195 and 10-1074 (6 MDa, Complex from Human immunodeficiency virus 1)
- Crf-1_ae t/f100 hiv-1 gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Heavy chain of 10-1074 (25 kDa, Protein from Homo sapiens)
- Heavy chain of 8anc195 (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Light chain of 8anc195 (23 kDa, Protein from Homo sapiens)
Cryo-EM structure of BG505 SOSIP.664 HIV-1 Env trimer in complex with temsavir, 8ANC195, and 10-1074
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8ttw
Q-score: 0.507
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ DOI: doi:10.1038/s41467-023-42500-2 Pubmed: 37872202 ]
- Antibody 8anc195 heavy chain (25 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp160 (54 kDa, Protein from Human immunodeficiency virus 1)
- Bg505 sosip.664 transmembrane protein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 1-[4-(benzenecarbonyl)piperazin-1-yl]-2-[4-methoxy-7-(3-methyl-1h-1,2,4-triazol-1-yl)-1h-pyrrolo[2,3-c]pyridin-3-yl]ethane-1,2-dione (473 Da, Ligand)
- Antibody 10-1074 light chain (23 kDa, Protein from Homo sapiens)
- Complex of bg505 sosip.664 with temsavir and the antibody fabs of 8anc195 and 10-1074 (Complex from Homo sapiens)
- Antibody 10-1074 heavy chain (25 kDa, Protein from Homo sapiens)
- Antibody 8anc195 light chain (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 8ekk
Q-score: 0.538
Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ
To Be Published
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8ekl
Q-score: 0.577
Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ
To Be Published
Clostridioides difficile binary toxin translocase CDTb double mutant - D623A D734A
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8ekm
Q-score: 0.502
Abeyawardhane DL, Adipietro KA, Ruiz RG, Varney KM, Rustandi RR, Silin VI, Pozharski E, Weber DJ
To Be Published
Cryo-EM structure of T/F100 SOSIP.664 HIV-1 Env trimer in complex with 8ANC195 and 10-1074
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8dok
Q-score: 0.484
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ DOI: doi:10.1038/s41467-023-42500-2 Pubmed: 37872202 ]
- Cryo-em structure of t/f100 sosip.664 hiv-1 env trimer in complex with 8anc195 and 10-1074 (6 MDa, Complex from Homo sapiens)
- Light chain of 10-1074 (23 kDa, Protein from Homo sapiens)
- Crf-1_ae t/f100 hiv-1 gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Heavy chain of 10-1074 (25 kDa, Protein from Homo sapiens)
- Crf-1_ae t/f100 env gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Heavy chain of 8anc195 (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Light chain of 8anc195 (23 kDa, Protein from Homo sapiens)
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8fsj
Q-score: 0.368
Metcalf MC, Janus BM, Yin R, Wang R, Guest JD, Pozharski E, Law M, Mariuzza RA, Toth EA, Pierce BG, Fuerst TR, Ofek G
Nat Commun (2023) 14 pp. 3980-3980 [ Pubmed: 37407593 DOI: doi:10.1038/s41467-023-39659-z ]
- Hepc74 heavy chain (27 kDa, Protein from Homo sapiens)
- Ar4a light chain (25 kDa, Protein from Homo sapiens)
- Hcv e2 ectodomain, synzip2 scaffold fusion (42 kDa, Protein from synthetic construct)
- Hcv e1e2 ectodomain in complex with ar4a, hepc74, and igh520 (250 kDa, Complex from Hepacivirus C)
- Ar4a heavy chain (31 kDa, Protein from Homo sapiens)
- Hepc74 light chain (23 kDa, Protein from Homo sapiens)
- Hcv e1 ectodomain, synzip1 scaffold fusion (22 kDa, Protein from synthetic construct)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 8czz
Q-score: 0.428
Prevost J, Chen Y, Zhou F, Tolbert WD, Gasser R, Medjahed H, Nayrac M, Nguyen DN, Gottumukkala S, Hessell AJ, Rao VB, Pozharski E, Huang RK, Matthies D, Finzi A, Pazgier M
Nat Commun (2023) 14 pp. 6710-6710 [ DOI: doi:10.1038/s41467-023-42500-2 Pubmed: 37872202 ]
- 1-[4-(benzenecarbonyl)piperazin-1-yl]-2-[4-methoxy-7-(3-methyl-1h-1,2,4-triazol-1-yl)-1h-pyrrolo[2,3-c]pyridin-3-yl]ethane-1,2-dione (473 Da, Ligand)
- Light chain of 8anc195 fab (23 kDa, Protein from Homo sapiens)
- Heavy chain of 10-1074 fab (25 kDa, Protein from Homo sapiens)
- Crf-1_ae t/f100 hiv-1 gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Cryo-em structure of t/f100 sosip.664 hiv-1 env trimer with lmhs mutations in complex with temsavir, 8anc195, and 10-1074 (6 MDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Crf01_ae t/f100 hiv-1 gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Heavy chain of 8anc195 fab (25 kDa, Protein from Homo sapiens)
- Light chain of 10-1074 fab (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 4.18 Å
EM Method: Single-particle
Fitted PDBs: 7rq6
Q-score: 0.33
Beaudoin-Bussieres G, Chen Y, Ullah I, Prevost J, Tolbert WD, Symmes K, Ding S, Benlarbi M, Gong SY, Tauzin A, Gasser R, Chatterjee D, Vezina D, Goyette G, Richard J, Zhou F, Stamatatos L, McGuire AT, Charest H, Roger M, Pozharski E, Kumar P, Mothes W, Uchil PD, Pazgier M, Finzi A
Cell Rep (2022) 38 pp. 110368-110368 [ Pubmed: 35123652 DOI: doi:10.1016/j.celrep.2022.110368 ]
- Cv3-13 fab heavy chain (24 kDa, Protein from Homo sapiens)
- Cv3-13 fab light chain (23 kDa, Protein from Homo sapiens)
- Cryo-em structure of sars-cov-2 spike in complex with non-neutralizing, ntd-directed human antibody cv3-13 (670 kDa, Complex from Severe acute respiratory syndrome coronavirus 2)
- Spike glycoprotein (138 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)