Electron Microscopy Data Bank

EMD-45671

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Subtomogram average of the Polar Tube Outer Filament layer from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 36.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

bioRxiv (2024) [ Pubmed: 39026755 DOI: doi:10.1101/2024.07.13.603322 ]

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMD-45672

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Subtomogram average of the Polar Tube Inner Filament layer from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 20.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

bioRxiv (2024) [ Pubmed: 39026755 DOI: doi:10.1101/2024.07.13.603322 ]

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMD-45673

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Subtomogram average of the Polar Tube Outer Filament Layer and Inner Filament layer from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 44.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

bioRxiv (2024) [ Pubmed: 39026755 DOI: doi:10.1101/2024.07.13.603322 ]

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMD-45674

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Subtomogram average of a whole Polar Tube cross-section from Encephalitozoon intestinalis microsporidian spores

Release date: Feb. 26, 2025
Resolution: 70.0 Å
EM Method: Subtomogram averaging

Usmani M, Coudray N, Riggi M, Raghu R, Ramchandani H, Bobe D, Kopylov M, Zhong ED, Iwasa JH, Ekiert DC, Bhabha G

bioRxiv (2024) [ Pubmed: 39026755 DOI: doi:10.1101/2024.07.13.603322 ]

Sample:

Encephalitozoon intestinalis microsporidian spores (Cell from Encephalitozoon intestinalis)

EMPIAR-12086

Release date: Jan. 16, 2025
Dataset size: 10.3 TB
Experiment type: EMDB

Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS

Mol. Cell (2023) 83 pp. 3852-3868 [ Pubmed: 37852256 DOI: 10.1016/j.molcel.2023.09.032 ]

Image sets:

[25,457 multi-frame micrographs composed of 40 frames each in TIFF format]

EMD-40464

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CCT G beta 5 complex intermediate state

Release date: Nov. 1, 2023
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8sgq
Q-score: 0.248

Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS

Mol Cell (2023) 83 pp. 3852-3868.e6 [ Pubmed: 37852256 DOI: doi:10.1016/j.molcel.2023.09.032 ]

Sample:

T-complex protein 1 subunit gamma (58 kDa, Protein from Homo sapiens)
Adenosine-5'-diphosphate (427 Da, Ligand)
T-complex protein 1 subunit theta (54 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit zeta (55 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit alpha (58 kDa, Protein from Homo sapiens)
Cct-gb5-phlp1 in intermediate state (947 MDa, Complex from Homo sapiens)
T-complex protein 1 subunit delta (56 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit eta, n-terminally processed (56 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit beta (56 kDa, Protein from Homo sapiens)

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EMD-40486

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CCT-G beta 5 complex closed state 7

Release date: Nov. 1, 2023
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8shf
Q-score: 0.537

Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS

Mol Cell (2023) 83 pp. 3852-3868.e6 [ Pubmed: 37852256 DOI: doi:10.1016/j.molcel.2023.09.032 ]

Sample:

T-complex protein 1 subunit gamma (58 kDa, Protein from Homo sapiens)
Guanine nucleotide-binding protein subunit beta-5 (43 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Water (18 Da, Ligand)
Phosducin-like protein (34 kDa, Protein from Homo sapiens)
Adenosine-5'-diphosphate (427 Da, Ligand)
T-complex protein 1 subunit zeta (57 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit alpha (58 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit delta (56 kDa, Protein from Homo sapiens)
Aluminum fluoride (83 Da, Ligand)
T-complex protein 1 subunit theta (58 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit beta (56 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit eta, n-terminally processed (57 kDa, Protein from Homo sapiens)
Cct-gb5-phlp1 in closed state 7 (Complex from Homo sapiens)

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EMD-40439

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Open state CCT-G beta 5 complex

Release date: Oct. 25, 2023
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 8sfe
Q-score: 0.3

Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS

Mol Cell (2023) 83 pp. 3852-3868.e6 [ Pubmed: 37852256 DOI: doi:10.1016/j.molcel.2023.09.032 ]

Sample:

T-complex protein 1 subunit gamma (58 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit eta (57 kDa, Protein from Homo sapiens)
Adenosine-5'-diphosphate (427 Da, Ligand)
T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit zeta (55 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit alpha (58 kDa, Protein from Homo sapiens)
Cct-gb5-phlp1 in open state (947 MDa, Complex from Homo sapiens)
T-complex protein 1 subunit theta (58 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit beta (56 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit delta (56 kDa, Protein from Homo sapiens)

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EMD-40440

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CCT G beta 5 complex closed state 0

Release date: Oct. 25, 2023
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8sff
Q-score: 0.543

Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS

Mol Cell (2023) 83 pp. 3852-3868.e6 [ Pubmed: 37852256 DOI: doi:10.1016/j.molcel.2023.09.032 ]

Sample:

T-complex protein 1 subunit gamma (58 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Water (18 Da, Ligand)
Phosducin-like protein (34 kDa, Protein from Homo sapiens)
Adenosine-5'-diphosphate (427 Da, Ligand)
T-complex protein 1 subunit zeta (57 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit alpha (58 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit delta (56 kDa, Protein from Homo sapiens)
Aluminum fluoride (83 Da, Ligand)
T-complex protein 1 subunit theta (58 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit beta (56 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit eta, n-terminally processed (57 kDa, Protein from Homo sapiens)
Cct-gb5-phlp1 complex in closed state 0 (Complex from Homo sapiens)

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EMD-40452

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CCT G beta 5 complex closed state 1

Release date: Oct. 25, 2023
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8sg8
Q-score: 0.543

Wang S, Sass MI, Kwon Y, Ludlam WG, Smith TM, Carter EJ, Gladden NE, Riggi M, Iwasa JH, Willardson BM, Shen PS

Mol Cell (2023) 83 pp. 3852-3868.e6 [ Pubmed: 37852256 DOI: doi:10.1016/j.molcel.2023.09.032 ]

Sample:

T-complex protein 1 subunit gamma (58 kDa, Protein from Homo sapiens)
Guanine nucleotide-binding protein subunit beta-5 (43 kDa, Protein from Homo sapiens)
Magnesium ion (24 Da, Ligand)
Water (18 Da, Ligand)
Phosducin-like protein (34 kDa, Protein from Homo sapiens)
Adenosine-5'-diphosphate (427 Da, Ligand)
T-complex protein 1 subunit zeta (57 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit epsilon (59 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit alpha (58 kDa, Protein from Homo sapiens)
Cct-gb5-phlp1 in intermediate state (947 MDa, Complex from Homo sapiens)
T-complex protein 1 subunit delta (56 kDa, Protein from Homo sapiens)
Aluminum fluoride (83 Da, Ligand)
T-complex protein 1 subunit theta (58 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit beta (56 kDa, Protein from Homo sapiens)
T-complex protein 1 subunit eta, n-terminally processed (57 kDa, Protein from Homo sapiens)

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