Resolution: 3.66 Å
EM Method: Single-particle
Fitted PDBs: 7uhy
Q-score: 0.415
Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, Chantranupong L, Kolibius J, Quast JP, Sabatini DM
Nature (2022) 607 pp. 610-616 [ Pubmed: 35831510 DOI: doi:10.1038/s41586-022-04939-z ]
- Unknown (698 Da, Protein from Homo sapiens)
- Human gator2 (1 MDa, Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Gator complex protein mios (100 kDa, Protein from Homo sapiens)
- Isoform b of nucleoporin seh1 (46 kDa, Protein from Homo sapiens)
- Gator complex protein wdr24 (88 kDa, Protein from Homo sapiens)
- Protein sec13 homolog (35 kDa, Protein from Homo sapiens)
- Gator complex protein wdr59 (109 kDa, Protein from Homo sapiens)
- Unknown (1 kDa, Protein from Homo sapiens)
Cryo-EM structure of the FLCN-FNIP2-Rag-Ragulator complex
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 6ulg
Q-score: 0.467
Shen K, Rogala KB, Chou HT, Huang RK, Yu Z, Sabatini DM
Cell (2019) 179 pp. 1319-1329.e8 [ Pubmed: 31704029 DOI: doi:10.1016/j.cell.2019.10.036 ]
- Nonameric complex of flcn-fnip2 with its substrate rag gtpases and the scaffolding protein complex ragulator (380 kDa, Complex from Homo sapiens)
- Ragulator complex protein lamtor4 (10 kDa, Protein from Homo sapiens)
- Folliculin (64 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor1 (17 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor5 (9 kDa, Protein from Homo sapiens)
- Folliculin-interacting protein 2 (122 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor3 (13 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor2 (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 6u62
Q-score: 0.513
Rogala KB, Gu X, Kedir JF, Abu-Remaileh M, Bianchi LF, Bottino AMS, Dueholm R, Niehaus A, Overwijn D, Fils AP, Zhou SX, Leary D, Laqtom NN, Brignole EJ, Sabatini DM
Science (2019) 366 pp. 468-475 [ DOI: doi:10.1126/science.aay0166 Pubmed: 31601708 ]
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor4 (10 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor1 (17 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor3 (17 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor5 (9 kDa, Protein from Homo sapiens)
- Ragulator complex protein lamtor2 (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Regulatory-associated protein of mtor (154 kDa, Protein from Homo sapiens)
- Raptor-rag-ragulator (Complex from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
Rogala KB, Gu X, Kedir JF, Abu-Remaileh M, Bianchi LF, Bottino AMS, Dueholm R, Niehaus A, Overwijn D, Fils AP, Zhou SX, Leary D, Laqtom NN, Brignole EJ, Sabatini DM
Science (2019) 366 pp. 468-475 [ DOI: doi:10.1126/science.aay0166 Pubmed: 31601708 ]
cryo-EM structure of mTORC1 bound to PRAS40-fused active RagA/C GTPases
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 6sb0
Q-score: 0.237
Anandapadamanaban M, Masson GR, Perisic O, Berndt A, Kaufman J, Johnson CM, Santhanam B, Rogala KB, Sabatini DM, Williams RL
Science (2019) 366 pp. 203-210 [ Pubmed: 31601764 DOI: doi:10.1126/science.aax3939 ]
- Raga/c (Complex from Homo sapiens)
- Proline-rich akt1 substrate 1 (27 kDa, Protein from Homo sapiens)
- Mtorc1 (Complex from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Regulatory-associated protein of mtor (149 kDa, Protein from Homo sapiens)
- Mtor,serine/threonine-protein kinase mtor,mtor,serine/threonine-protein kinase mtor (287 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)
- Cryoem structure of mtorc1 bound to raga/c complex (1 MDa, Complex)
cryo-EM structure of mTORC1 bound to active RagA/C GTPases
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 6sb2
Q-score: 0.211
Anandapadamanaban M, Masson GR, Perisic O, Berndt A, Kaufman J, Johnson CM, Santhanam B, Rogala KB, Sabatini DM, Williams RL
Science (2019) 366 pp. 203-210 [ Pubmed: 31601764 DOI: doi:10.1126/science.aax3939 ]
- Mtor,serine/threonine-protein kinase mtor,mtor,serine/threonine-protein kinase mtor (287 kDa, Protein from Homo sapiens)
- Raga/c (Complex from Homo sapiens)
- Mtorc1 (Complex from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Cryo-em structure of mtorc1 bound to active raga/c complex (1 MDa, Complex)
- Ras-related gtp-binding protein c (44 kDa, Protein from Homo sapiens)
- Regulatory-associated protein of mtor (149 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ras-related gtp-binding protein a (36 kDa, Protein from Homo sapiens)
- Target of rapamycin complex subunit lst8 (35 kDa, Protein from Homo sapiens)