Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation I
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6n57
Q-score: 0.447
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Escherichia coli sigma70-holoenzyme with trar in conformation i (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation II
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 6n58
Q-score: 0.443
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]
- Magnesium ion (24 Da, Ligand)
- Protein trar (8 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Escherichia coli sigma70-holoenzyme with trar in conformation ii (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Cryo-EM structure of Escherichia coli RNAP polymerase bound to rpsTP2 promoter DNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6oul
Q-score: 0.46
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]
- Dna-directed rna polymerase subunit beta' (158 kDa, Protein from Escherichia coli)
- Cryo-em structure of escherichia coli rnap polymerase bound to rpstp2 promoter dna (Complex from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Template strand of rpstp2 dna promoter (26 kDa, DNA from Escherichia coli K-12)
- Chapso (631 Da, Ligand)
- Non-template strand of rpstp2 dna promoter (26 kDa, DNA from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Cryo-EM structure of Escherichia coli RNAP polymerase holoenzyme
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]
Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation III
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]
Cryo-EM structure of Escherichia coli RNAP polymerase bound to DNA promoter rpsTP2 (class 2)
Chen J, Gopalkrishnan S, Chiu C, Chen AY, Campbell EA, Gourse RL, Ross W, Darst SA
eLife (2019) 8 [ Pubmed: 31841111 DOI: doi:10.7554/eLife.49375 ]