CryoEM Structure of HCA2-Gi1 in complex with MK-1903
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8utd
Q-score: 0.347
Kang HJ, Krumm BE, Tassou A, Geron M, DiBerto JF, Kapolka NJ, Gumpper RH, Sakamoto K, Dewran Kocak D, Olsen RHJ, Huang XP, Zhang S, Huang KL, Zaidi SA, Nguyen MT, Jo MJ, Katritch V, Fay JF, Scherrer G, Roth BL
Cell (2024) 187 pp. 7433-7449.e20 [ DOI: doi:10.1016/j.cell.2024.11.001 Pubmed: 39631393 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- (4ar,5ar)-4,4a,5,5a-tetrahydro-1h-cyclopropa[4,5]cyclopenta[1,2-c]pyrazole-3-carboxylic acid (164 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Human hca2-gai1 in complex with mk1903 (Complex from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- Hydroxycarboxylic acid receptor 2 (42 kDa, Protein from Homo sapiens)
CryoEM Structure of HCA2 DREADD Gi1 in complex with FCH-2296413
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8uuj
Q-score: 0.53
Kang HJ, Krumm BE, Tassou A, Geron M, DiBerto JF, Kapolka NJ, Gumpper RH, Sakamoto K, Dewran Kocak D, Olsen RHJ, Huang XP, Zhang S, Huang KL, Zaidi SA, Nguyen MT, Jo MJ, Katritch V, Fay JF, Scherrer G, Roth BL
Cell (2024) 187 pp. 7433-7449.e20 [ DOI: doi:10.1016/j.cell.2024.11.001 Pubmed: 39631393 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mouse hcad-gai1 in complex with fch-2296413 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- (3m,4ar,5ar)-3-(1h-tetrazol-5-yl)-4,4a,5,5a-tetrahydro-1h-cyclopropa[4,5]cyclopenta[1,2-c]pyrazole (188 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Lama glama)
- Hydroxycarboxylic acid receptor 2 (41 kDa, Protein from Mus musculus)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8uwl
Q-score: 0.572
Lyu J, Kapolka N, Gumpper R, Alon A, Wang L, Jain MK, Barros-Alvarez X, Sakamoto K, Kim Y, DiBerto J, Kim K, Glenn IS, Tummino TA, Huang S, Irwin JJ, Tarkhanova OO, Moroz Y, Skiniotis G, Kruse AC, Shoichet BK, Roth BL
Science (2024) 384 pp. eadn6354-eadn6354 [ Pubmed: 38753765 DOI: doi:10.1126/science.adn6354 ]
- N,n-diethyl-n'-[(8alpha)-6-methyl-9,10-didehydroergolin-8-yl]urea (338 Da, Ligand)
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 5-ht2ar bound to lisuride in complex with heterotrimeric mini-gq protein and single-chain variable fragment (scfv16) (Complex from Homo sapiens)
- G protein subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2a (38 kDa, Protein from Homo sapiens)
5HT2AR-miniGq heterotrimer in complex with a novel agonist obtained from large scale docking
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8v6u
Q-score: 0.54
Lyu J, Kapolka N, Gumpper R, Alon A, Wang L, Jain MK, Barros-Alvarez X, Sakamoto K, Kim Y, DiBerto J, Kim K, Glenn IS, Tummino TA, Huang S, Irwin JJ, Tarkhanova OO, Moroz Y, Skiniotis G, Kruse AC, Shoichet BK, Roth BL
Science (2024) 384 pp. eadn6354-eadn6354 [ Pubmed: 38753765 DOI: doi:10.1126/science.adn6354 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G protein alpha-subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
- 4-{(3r)-1-[(1r)-1-(pyrimidin-2-yl)ethyl]piperidin-3-yl}phenol (283 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2a (38 kDa, Protein from Homo sapiens)
- Active state complex of 5ht2ar with mini-gq heterotrimer stabilized by scfv16 (Complex from Homo sapiens)
Cryo-EM structure of the human GS-GN complex in the inhibited state
Marr L, Biswas D, Daly LA, Browning C, Vial SCM, Maskell DP, Hudson C, Bertrand JA, Pollard J, Ranson NA, Khatter H, Eyers CE, Sakamoto K, Zeqiraj E
Nat Commun (2022) 13 [ Pubmed: 35690592 DOI: 10.1038/s41467-022-31109-6 ]
Image sets:
Cryo-EM structure of the human GS-GN complex in the inhibited state
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 7zbn
Q-score: 0.62
Marr L, Biswas D, Daly LA, Browning C, Vial SCM, Maskell DP, Hudson C, Bertrand JA, Pollard J, Ranson NA, Khatter H, Eyers CE, Sakamoto K, Zeqiraj E
Nat Commun (2022) 13 pp. 3372-3372 [ Pubmed: 35690592 DOI: doi:10.1038/s41467-022-31109-6 ]
- Isoform gn-1 of glycogenin-1 (37 kDa, Protein from Homo sapiens)
- Glycogen synthase-glycogenin complex (485 kDa, Complex from Homo sapiens)
- Glycogen [starch] synthase, muscle (83 kDa, Protein from Homo sapiens)
- Glycogen [starch] synthase, muscle (83 kDa, Protein from Homo sapiens)
RNA-targeting by the Type III-A CRISPR-Cas Csm complex of Thermus thermophilus
Staals RHJ, Zhu Y, Taylor DW, Kornfeld JE, Sharma K, Barendregt A, Koehorst JJ, Vlot M, Neupane N, Varossieau K, Sakamoto K, Suzuki T, Dohmae N, Yokoyama S, Schaap PJ, Urlaub H, Heck AJR, Nogales E, Doudna JA, Shinkai A, van der Oost J
Mol Cell (2014) 56 pp. 518-530 [ DOI: doi:10.1016/j.molcel.2014.10.005 Pubmed: 25457165 ]
- Csm2 (16 kDa, Protein from Thermus thermophilus)
- Csm3 (27 kDa, Protein from Thermus thermophilus)
- Csm4 (32 kDa, Protein from Thermus thermophilus)
- Csm1 (90 kDa, Protein from Thermus thermophilus)
- Type iii-a crispr-cas csm complex of thermus thermophilus (426 kDa, Sample)
- Crispr rna (14 kDa, RNA from Thermus thermophilus)
- Csm5 (45 kDa, Protein from Thermus thermophilus)
Electron microscopy of the negatively-stained Cmr complex from Thermus thermophilus HB8.
Staals RH, Agari Y, Maki-Yonekura S, Zhu Y, Taylor DW, van Duijn E, Barendregt A, Vlot M, Koehorst JJ, Sakamoto K, Masuda A, Dohmae N, Schaap PJ, Doudna JA, Heck AJ, Yonekura K, van der Oost J, Shinkai A
Mol Cell (2013) 52 pp. 135-145 [ DOI: doi:10.1016/j.molcel.2013.09.013 Pubmed: 24119403 ]
- Cmr1 (40 kDa, Protein from Thermus thermophilus)
- Cmr2 (65 kDa, Protein from Thermus thermophilus)
- Cmr4 (30 kDa, Protein from Thermus thermophilus)
- Cmr3 (40 kDa, Protein from Thermus thermophilus)
- Cmr5 (10 kDa, Protein from Thermus thermophilus)
- Cmr6 (40 kDa, Protein from Thermus thermophilus)
- Cmr complex from thermus thermophilus (400 kDa, Sample)
- Crrna (20 kDa, RNA from Thermus thermophilus)
Structure and activity of an RNA-targeting Type III-B CRISPER-Cas complex
Staals RH, Agari Y, Maki-Yonekura S, Zhu Y, Taylor DW, van Duijn E, Barendregt A, Vlot M, Koehorst JJ, Sakamoto K, Masuda A, Dohmae N, Schaap PJ, Doudna JA, Heck AJ, Yonekura K, van der Oost J, Shinkai A
Mol Cell (2013) 52 pp. 135-145 [ DOI: doi:10.1016/j.molcel.2013.09.013 Pubmed: 24119403 ]
- Type iii-b crispr-cas complex of thermus thermophilus (365 kDa, Sample)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)