Release Complex: BAM bound EspP and Extended SurA
Resolution: 5.2 Å
EM Method: Single-particle
Fitted PDBs: 8qpu
Q-score: 0.338
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Chaperone sura (49 kDa, Protein from Escherichia coli)
- Serine protease espp (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Release complex: bam bound espp and compact sura (290 kDa, Complex from Escherichia coli)
Release Complex: BAM bound EspP and Compact SurA
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 8q0g
Q-score: 0.379
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Chaperone sura (49 kDa, Protein from Escherichia coli)
- Serine protease espp (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Release complex: bam bound espp and compact sura (290 kDa, Complex from Escherichia coli)
Wait Complex: Lateral open BAM bound Compact SurA
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8pz1
Q-score: 0.449
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Chaperone sura (49 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Wait complex: lateral open bam bound compact sura (250 kDa, Complex from Escherichia coli)
Wait Complex: Lateral open BAM bound Extended SurA
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8pz2
Q-score: 0.46
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Wait complex: lateral open bam bound extended sura (250 kDa, Complex from Escherichia coli)
- Chaperone sura (49 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
Wait Complex: BAM bound Darobactin-B and Compact SurA
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8pzu
Q-score: 0.437
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Chaperone sura (49 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Wait complex: bam bound darobactin-b and compact sura (250 kDa, Complex from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Darobactin-b (1 kDa, Protein from Photorhabdus heterorhabditis)
Wait Complex: BAM bound Darobactin-B and Extended SurA
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8pzv
Q-score: 0.548
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Chaperone sura (49 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Wait complex: bam bound darobactin-b and extended sura (250 kDa, Complex from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Darobactin-b (1 kDa, Protein from Photorhabdus heterorhabditis)
Release Complex: BAM bound EspP (SurA released)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8qp5
Q-score: 0.368
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Release complex: bam bound espp and sura released (290 kDa, Complex from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Chaperone sura,serine protease espp (89 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
Handover Complex: BAM bound OmpX and extended SurA
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8qpv
Q-score: 0.438
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Handover complex: bam bound ompx and extended sura (270 kDa, Complex)
- Chaperone sura,outer membrane protein x (67 kDa, Protein from Escherichia coli)
- Chaperone sura,outer membrane protein x (Complex from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factors (Complex from Escherichia coli)
Arrival Complex: Lateral open BAM bound extended SurA plus OmpX
Resolution: 5.3 Å
EM Method: Single-particle
Fitted PDBs: 8qpw
Q-score: 0.332
Fenn KL, Horne JE, Crossley JA, Bohringer N, Horne RJ, Schaberle TF, Calabrese AN, Radford SE, Ranson NA
Nat Commun (2024) 15 pp. 7612-7612 [ Pubmed: 39218969 DOI: doi:10.1038/s41467-024-51358-x ]
- Chaperone sura (49 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Arrival complex: lateral open bam bound extended sura plus ompx (270 kDa, Complex from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Ompx (20 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
E. coli BAM complex (BamABCDE) bound to darobactin B
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8bvq
Q-score: 0.444
Haysom SF, Machin J, Whitehouse JM, Horne JE, Fenn K, Ma Y, El Mkami H, Bohringer N, Schaberle TF, Ranson NA, Radford SE, Pliotas C
Angew Chem Int Ed Engl (2023) 62 pp. e202218783-e202218783 [ Pubmed: 37162386 DOI: doi:10.1002/anie.202218783 ]
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- E. coli bam complex (bamabcde) bound to darobactin b (200 kDa, Complex from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Darobactin-b (1 kDa, Protein from Photorhabdus heterorhabditis)