Type I-F CRISPR-Csy complex with its inhibitor AcrF9
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 6vqv
Q-score: 0.495
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
PNAS (2020) 117 pp. 7176-7182 [ DOI: doi:10.1073/pnas.1922638117 Pubmed: 32170016 ]
- Crrna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-csy complex with its inhibitor acrf9 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
- Acrf9 (7 kDa, Protein from Pseudomonas aeruginosa)
Type I-F CRISPR-Csy complex with its inhibitor AcrF8
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 6vqw
Q-score: 0.462
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
PNAS (2020) 117 pp. 7176-7182 [ DOI: doi:10.1073/pnas.1922638117 Pubmed: 32170016 ]
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Acrf8 (10 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-csy complex with its inhibitor acrf8 (350 kDa, Complex from Pseudomonas aeruginosa)
- Type i-f crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)
- Crrna (40-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
Type I-F CRISPR-Csy complex with its inhibitor AcrF6
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 6vqx
Q-score: 0.433
Zhang K, Wang S, Li S, Zhu Y, Pintilie GD, Mou TC, Schmid MF, Huang Z, Chiu W
PNAS (2020) 117 pp. 7176-7182 [ DOI: doi:10.1073/pnas.1922638117 Pubmed: 32170016 ]
- Crrna (60-mer) (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy1 (49 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-associated protein csy2 (36 kDa, Protein from Pseudomonas aeruginosa)
- Acrf6 (10 kDa, Protein from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (39 kDa, Protein from Pseudomonas aeruginosa)
- Type i-f crispr-csy complex with its inhibitor acrf6 (350 kDa, Complex from Pseudomonas aeruginosa)
- Crispr-associated endonuclease cas6/csy4 (21 kDa, Protein from Pseudomonas aeruginosa)
Asymmetric structure of AcrAB-TolC tripartite multi drug efflux pump
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 5o66
Q-score: 0.24
Wang Z, Fan G, Hryc CF, Blaza JN, Serysheva II, Schmid MF, Chiu W, Luisi BF, Du D
eLife (2017) 6 [ DOI: doi:10.7554/eLife.24905 Pubmed: 28355133 ]
- 6-[2-(3,4-dimethoxyphenyl)ethylsulfanyl]-8-[4-(2-methoxyethyl)piperazin-1-yl]-3,3-dimethyl-1,4-dihydropyrano[3,4-c]pyridine-5-carbonitrile (526 Da, Ligand)
- Outer membrane protein tolc (53 kDa, Protein from Escherichia coli (strain K12))
- Multidrug efflux pump accessory protein acrz (5 kDa, Protein from Escherichia coli (strain K12))
- Multidrug efflux pump subunit acra (39 kDa, Protein from Escherichia coli (strain K12))
- Acrabz-tolc (Complex from Escherichia coli K12)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli (strain K12))
multi-drug efflux; membrane transport; RND superfamily; Drug resistance
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 5ng5
Q-score: 0.326
Wang Z, Fan G, Hryc CF, Blaza JN, Serysheva II, Schmid MF, Chiu W, Luisi BF, Du D
eLife (2017) 6 [ DOI: doi:10.7554/eLife.24905 Pubmed: 28355133 ]
- Multidrug efflux pump accessory protein acrz (5 kDa, Protein from Escherichia coli)
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli)
- Acrabtolc in apo state (Complex from Escherichia coli)
- 6-[2-(3,4-dimethoxyphenyl)ethylsulfanyl]-8-[4-(2-methoxyethyl)piperazin-1-yl]-3,3-dimethyl-1,4-dihydropyrano[3,4-c]pyridine-5-carbonitrile (526 Da, Ligand)
- Multidrug efflux pump subunit acra (39 kDa, Protein from Escherichia coli)
- Outer membrane protein tolc (53 kDa, Protein from Escherichia coli)
multi-drug efflux; membrane transport; RND superfamily; Drug resistance
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 5v5s
Q-score: 0.193
Wang Z, Fan G, Hryc CF, Blaza JN, Serysheva II, Schmid MF, Chiu W, Luisi BF, Du D
eLife (2017) 6 [ DOI: doi:10.7554/eLife.24905 Pubmed: 28355133 ]
- Multidrug efflux pump subunit acrb (113 kDa, Protein from Escherichia coli )
- Multidrug efflux pump subunit acra (42 kDa, Protein from Escherichia coli)
- Acrabtolc in apo state (Complex from Escherichia coli)
- Outer membrane protein tolc (48 kDa, Protein from Escherichia coli)
Bacteriophage P22 mature virion capsid protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 5uu5
Q-score: 0.548
Hryc CF, Chen DH, Afonine PV, Jakana J, Wang Z, Haase-Pettingell C, Jiang W, Adams PD, King JA, Schmid MF, Chiu W
PNAS (2017) 114 pp. 3103-3108 [ DOI: doi:10.1073/pnas.1621152114 Pubmed: 28270620 ]
- Major capsid protein (46 kDa, Protein from Salmonella phage P22)
- Enterobacteria phage p22 (327 MDa, Virus from Enterobacteria phage P22)