Resolution: 3.23 Å
EM Method: Single-particle
Fitted PDBs: 8r60
Q-score: 0.406
Keown J, Baazaoui A, Sebesta M, Stefl R, Carrique L, Fodor E, Grimes JM
J Virol (2024) 98 pp. e0013824-e0013824 [ Pubmed: 38563748 DOI: doi:10.1128/jvi.00138-24 ]
- Rna (5'-r(p*ap*gp*up*ap*gp*ap*ap*ap*cp*ap*ap*gp*gp*cp*c)-3') (4 kDa, RNA from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Rna (5'-r(p*gp*gp*cp*cp*up*gp*cp*u)-3') (5 kDa, RNA from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Rna polymerase ii 4 repeat peptide with serine5 phosphorylation (3 kDa, Protein from Homo sapiens)
- Polymerase basic protein 2 (102 kDa, Protein from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- 1918 h1n1 influenza virus heterotrimer in complex with a 4 repeat serine 5 phosphorylated peptide from polii ctd (Complex from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
Resolution: 4.23 Å
EM Method: Single-particle
Fitted PDBs: 8r65
Q-score: 0.18
Keown J, Baazaoui A, Sebesta M, Stefl R, Carrique L, Fodor E, Grimes JM
J Virol (2024) 98 pp. e0013824-e0013824 [ Pubmed: 38563748 DOI: doi:10.1128/jvi.00138-24 ]
- Rna (5'-r(p*ap*gp*up*ap*gp*ap*ap*ap*cp*ap*ap*gp*gp*cp*c)-3') (4 kDa, RNA from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Rna (5'-r(p*gp*gp*cp*cp*up*gp*cp*u)-3') (5 kDa, RNA from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- 1918 h1n1 viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated polii peptide with ordered pb2 c-terminal domains (Complex from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Rna polymerase ii 4 repeat peptide with serine5 phosphorylation (3 kDa, Protein from Homo sapiens)
- Polymerase basic protein 2 (102 kDa, Protein from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Brevig Mission/1/1918(H1N1)))
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate and TARBP2 subunit
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 7zpj
Q-score: -0.01
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from Mus musculus)
- Risc-loading complex subunit tarbp2 isoform 1 (39 kDa, Protein from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Pre-dicing state of mouse somatic dicer with tarbp2 (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with pre-mir-15a and tarbp2 (Complex)
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate
Resolution: 4.19 Å
EM Method: Single-particle
Fitted PDBs: 7yym
Q-score: 0.192
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- 59-nt precursor of mir-15a (19 kDa, RNA from synthetic construct)
- Pre-dicing state of mouse somatic dicer (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with pre-mir-15a (Complex)
- Pre-mir-15a (Complex from synthetic construct)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
Mammalian Dicer in the dicing state with pre-miR-15a substrate
Resolution: 6.21 Å
EM Method: Single-particle
Fitted PDBs: 7yyn
Q-score: 0.133
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Dicing state of mouse oocyte dicer with pre-mir-15a (Complex)
- Isoform 2 of endoribonuclease dicer (Complex from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- 59-nt precursor of mir-15a (Complex from Mus musculus)
- Isoform 2 of endoribonuclease dicer (200 kDa, Protein from Mus musculus)
Mouse endoribonuclease Dicer (composite structure)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 7yz4
Q-score: 0.293
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Free form of mouse somatic dicer (Cellular component from Mus musculus)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
Mammalian Dicer in the "dicing state" with pre-miR-15a substrate
Resolution: 5.91 Å
EM Method: Single-particle
Fitted PDBs: 7zpi
Q-score: 0.106
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from Mus musculus)
- Dicing state of mouse somatic dicer (Complex from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Dicing state of mouse somatic dicer with pre-mir-15a (Complex)