CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7ul2
Q-score: 0.645
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- 2-[[1-(7-chloranylquinolin-4-yl)-5-(2,6-dimethoxyphenyl)pyrazol-3-yl]carbonylamino]adamantane-2-carboxylic acid (587 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ntsr1 (Complex from Homo sapiens)
- Complex of ntsr1 and nb6 (Complex from Homo sapiens)
- Neurotensin receptor 1 (47 kDa, Protein from Homo sapiens)
CryoEM Structure of Inactive H2R Bound to Famotidine, Nb6M, and NabFab
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ul3
Q-score: 0.36
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nb6m (Complex from Lama glama)
- Nabfab (Complex from synthetic construct)
- H2r (Complex from Homo sapiens)
- Histamine h2 receptor (44 kDa, Protein from Homo sapiens)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nanobody 6m (14 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Complex of h2r, nb6m, and nabfab (Complex from Homo sapiens)
- Famotidine (339 Da, Ligand)
CryoEM Structure of Inactive MOR Bound to Alvimopan and Mb6
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ul4
Q-score: 0.563
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Mb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- Complex of mor and nb6 (Complex from Mus musculus)
- Mor (Complex from Mus musculus)
- N-[(2s)-2-{[(3r,4r)-4-(3-hydroxyphenyl)-3,4-dimethylpiperidin-1-yl]methyl}-3-phenylpropanoyl]glycine (424 Da, Ligand)
- Mu-type opioid receptor (47 kDa, Protein from Mus musculus)
- Megabody 6 (55 kDa, Protein from synthetic construct)
CryoEM Structure of Inactive SSTR2 bound to Nb6
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ul5
Q-score: 0.562
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Complex of sstr2 and nb6 (Complex from Homo sapiens)
- Somatostatin receptor type 2 (46 kDa, Protein from Homo sapiens)
- Sstr2 (Complex from Homo sapiens)
LPHN3 (ADGRL3) 7TM domain bound to tethered agonist in complex with G protein heterotrimer
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7sf7
Q-score: 0.479
Barros-Alvarez X, Nwokonko RM, Vizurraga A, Matzov D, He F, Papasergi-Scott MM, Robertson MJ, Panova O, Yardeni EH, Seven AB, Kwarcinski FE, Su H, Peroto MC, Meyerowitz JG, Shalev-Benami M, Tall GG, Skiniotis G
Nature (2022) 604 pp. 757-762 [ Pubmed: 35418682 DOI: doi:10.1038/s41586-022-04575-7 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Latrophilin 3 (adgrl3) 7tm domain bound to tethered agonist in complex with mini-g13 protein (Complex from Homo sapiens)
- Isoform 1 of adhesion g protein-coupled receptor l3 (34 kDa, Protein from Homo sapiens)
GPR56 (ADGRG1) 7TM domain bound to tethered agonist in complex with G protein heterotrimer
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7sf8
Q-score: 0.544
Barros-Alvarez X, Nwokonko RM, Vizurraga A, Matzov D, He F, Papasergi-Scott MM, Robertson MJ, Panova O, Yardeni EH, Seven AB, Kwarcinski FE, Su H, Peroto MC, Meyerowitz JG, Shalev-Benami M, Tall GG, Skiniotis G
Nature (2022) 604 pp. 757-762 [ Pubmed: 35418682 DOI: doi:10.1038/s41586-022-04575-7 ]
- Isoform 2 of adhesion g-protein coupled receptor g1 (34 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G protein subunit 13 (gi2-mini-g13 chimera) (26 kDa, Protein from Homo sapiens)
- Gpr56 (adgrg1) 7tm domain bound to tethered agonist in complex with mini-g13 protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
CryoEM Structure of Full-Length mGlu2 in Inactive-State Bound to Antagonist LY341495
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 7mtq
Q-score: 0.39
Seven AB, Barros-Alvarez X, de Lapeyriere M, Papasergi-Scott MM, Robertson MJ, Zhang C, Nwokonko RM, Gao Y, Meyerowitz JG, Rocher JP, Schelshorn D, Kobilka BK, Mathiesen JM, Skiniotis G
Nature (2021) 595 pp. 450-454 [ DOI: doi:10.1038/s41586-021-03680-3 Pubmed: 34194039 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Metabotropic glutamate receptor 2 (93 kDa, Protein from Homo sapiens)
- 2-[(1s,2s)-2-carboxycyclopropyl]-3-(9h-xanthen-9-yl)-d-alanine (353 Da, Ligand)
- Full-length mglu2 in inactive-state (191 kDa, Complex from Homo sapiens)
CryoEM Structure of Full-Length mGlu2 Bound to Ago-PAM ADX55164 and Glutamate
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7mtr
Q-score: 0.427
Seven AB, Barros-Alvarez X, de Lapeyriere M, Papasergi-Scott MM, Robertson MJ, Zhang C, Nwokonko RM, Gao Y, Meyerowitz JG, Rocher JP, Schelshorn D, Kobilka BK, Mathiesen JM, Skiniotis G
Nature (2021) 595 pp. 450-454 [ DOI: doi:10.1038/s41586-021-03680-3 Pubmed: 34194039 ]
- Metabotropic glutamate receptor 2 (93 kDa, Protein from Homo sapiens)
- Full-length mglu2 in ago-pam and l-glutamate bound-state (191 kDa, Complex from Homo sapiens)
- Glutamic acid (147 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 2-methoxy-6-propyl-n-(2-{4-[(1h-tetrazol-5-yl)methoxy]phenyl}ethyl)thieno[2,3-d]pyrimidin-4-amine (425 Da, Ligand)
CryoEM Structure of mGlu2 - Gi Complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7mts
Q-score: 0.54
Seven AB, Barros-Alvarez X, de Lapeyriere M, Papasergi-Scott MM, Robertson MJ, Zhang C, Nwokonko RM, Gao Y, Meyerowitz JG, Rocher JP, Schelshorn D, Kobilka BK, Mathiesen JM, Skiniotis G
Nature (2021) 595 pp. 450-454 [ DOI: doi:10.1038/s41586-021-03680-3 Pubmed: 34194039 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mglu2 - heterotrimeric gi complex (277 kDa, Complex from Homo sapiens)
- Metabotropic glutamate receptor 2 (93 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Glutamic acid (147 Da, Ligand)
- 2-methoxy-6-propyl-n-(2-{4-[(1h-tetrazol-5-yl)methoxy]phenyl}ethyl)thieno[2,3-d]pyrimidin-4-amine (425 Da, Ligand)
Asymmetric Activation of the Calcium Sensing Receptor Homodimer
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7m3e
Q-score: 0.563
Gao Y, Robertson MJ, Rahman SN, Seven AB, Zhang C, Meyerowitz JG, Panova O, Hannan FM, Thakker RV, Brauner-Osborne H, Mathiesen JM, Skiniotis G
Nature (2021) 595 pp. 455-459 [ Pubmed: 34194040 DOI: doi:10.1038/s41586-021-03691-0 ]
- Phosphate ion (94 Da, Ligand)
- Extracellular calcium-sensing receptor (101 kDa, Protein from Homo sapiens)
- Tryptophan (204 Da, Ligand)
- 2-chloro-6-[(2r)-2-hydroxy-3-{[2-methyl-1-(naphthalen-2-yl)propan-2-yl]amino}propoxy]benzonitrile (408 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human extracellular calcium-sensing receptor complexed with negative allosteric modulator nps-2143 under high calcium high trp condition (200 MDa, Complex from Homo sapiens)