Levit A, Kaplan AL, Confair DN, Kim K, Barros-Álvarez X, Rodriguiz RM, Yang Y, Kweon OS, Che T, McCorvy JD, Kamber DN, Phelan JP, Martins LC, Pogorelov VM, DiBerto JF, Slocum ST, Huang XP, Kumar JM, Robertson MJ, Panova O, Seven AB, Wetsel AQ, Wetsel WC, Irwin JJ, Skiniotis G, Shoichet BK, Roth BL, Ellman JA
Nature (2022) 610 pp. 582-591 [ Pubmed: 36171289 DOI: 10.1038/s41586-022-05258-z ]
Image sets:
5-HT2B receptor bound to LSD obtained by cryo-electron microscopy (cryoEM)
Cao C, Barros-Álvarez X, Zhang S, Kim K, Dämgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Hüttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154-3167.e7 [ DOI: 10.1016/j.neuron.2022.08.006 Pubmed: 36087581 ]
Image sets:
CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t2g
Q-score: 0.672
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ DOI: doi:10.1038/s41589-022-01208-y Pubmed: 36411392 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Mu-opioid receptor / gi protein complex bound to mitragynine pseudoindoxyl (mp). (Complex from Mus musculus)
- Soluble cytochrome b562,mu-type opioid receptor (55 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mitragynine pseudoindoxyl (414 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7t2h
Q-score: 0.528
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ DOI: doi:10.1038/s41589-022-01208-y Pubmed: 36411392 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-opioid receptor - gi protein complex bound to lofentanil (lft) (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Lofentanil (408 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
5-HT2B receptor bound to LSD obtained by cryo-electron microscopy (cryoEM)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7srq
Q-score: 0.446
Cao C, Barros-Alvarez X, Zhang S, Kim K, Damgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Huttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154- [ Pubmed: 36087581 DOI: doi:10.1016/j.neuron.2022.08.006 ]
- 5-hydroxytryptamine receptor 2b (35 kDa, Protein from Homo sapiens)
- (8alpha)-n,n-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide (323 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 5-ht2b receptor bound to lsd (Complex from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7srr
Q-score: 0.564
Cao C, Barros-Alvarez X, Zhang S, Kim K, Damgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Huttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154- [ Pubmed: 36087581 DOI: doi:10.1016/j.neuron.2022.08.006 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 5-ht2b receptor bound to lsd in complex with heterotrimeric mini-gq protein and single-chain variable fragment (scfv16) (Complex from Homo sapiens)
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2b (41 kDa, Protein from Homo sapiens)
- (8alpha)-n,n-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide (323 Da, Ligand)
- G protein subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7srs
Q-score: 0.523
Cao C, Barros-Alvarez X, Zhang S, Kim K, Damgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Huttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154- [ Pubmed: 36087581 DOI: doi:10.1016/j.neuron.2022.08.006 ]
- Anti-5ht2br fab heavy chain (23 kDa, Protein from Mus musculus)
- Fab30 light chain (11 kDa, Protein from Mus musculus)
- Isoform 1b of beta-arrestin-1 (41 kDa, Protein from Homo sapiens)
- 5-ht2b receptor bound to lsd in complex with beta-arrestin1 (Complex)
- Anti-5ht2br fab light chain (22 kDa, Protein from Mus musculus)
- Fab30 heavy chain (13 kDa, Protein from Mus musculus)
- (8alpha)-n,n-diethyl-6-methyl-9,10-didehydroergoline-8-carboxamide (323 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 5-hydroxytryptamine receptor 2b (37 kDa, Protein from Homo sapiens)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 7ran
Q-score: 0.49
Kaplan AL, Confair DN, Kim K, Barros-Alvarez X, Rodriguiz RM, Yang Y, Kweon OS, Che T, McCorvy JD, Kamber DN, Phelan JP, Martins LC, Pogorelov VM, DiBerto JF, Slocum ST, Huang XP, Kumar JM, Robertson MJ, Panova O, Seven AB, Wetsel AQ, Wetsel WC, Irwin JJ, Skiniotis G, Shoichet BK, Roth BL, Ellman JA
Nature (2022) 610 pp. 582-591 [ Pubmed: 36171289 DOI: doi:10.1038/s41586-022-05258-z ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 5-ht2ar bound to a tetrahydropyridine agonist (r-69) in complex with heterotrimeric mini-gq protein and single-chain variable fragment (scfv16) (Complex from Homo sapiens)
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- G protein subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2a (38 kDa, Protein from Homo sapiens)
- (3r)-3-methyl-5-(1h-pyrrolo[2,3-b]pyridin-3-yl)-1,2,3,6-tetrahydropyridin-1-ium (214 Da, Ligand)