CryoEM structure of mu-opioid receptor - Gi protein complex bound to mitragynine pseudoindoxyl (MP)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t2g
Q-score: 0.672
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ DOI: doi:10.1038/s41589-022-01208-y Pubmed: 36411392 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Mu-opioid receptor / gi protein complex bound to mitragynine pseudoindoxyl (mp). (Complex from Mus musculus)
- Soluble cytochrome b562,mu-type opioid receptor (55 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mitragynine pseudoindoxyl (414 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7t2h
Q-score: 0.528
Qu Q, Huang W, Aydin D, Paggi JM, Seven AB, Wang H, Chakraborty S, Che T, DiBerto JF, Robertson MJ, Inoue A, Suomivuori CM, Roth BL, Majumdar S, Dror RO, Kobilka BK, Skiniotis G
Nat Chem Biol (2022) [ DOI: doi:10.1038/s41589-022-01208-y Pubmed: 36411392 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Mu-opioid receptor - gi protein complex bound to lofentanil (lft) (Complex from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Lofentanil (408 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mu-type opioid receptor (39 kDa, Protein from Mus musculus)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 7ran
Q-score: 0.49
Kaplan AL, Confair DN, Kim K, Barros-Alvarez X, Rodriguiz RM, Yang Y, Kweon OS, Che T, McCorvy JD, Kamber DN, Phelan JP, Martins LC, Pogorelov VM, DiBerto JF, Slocum ST, Huang XP, Kumar JM, Robertson MJ, Panova O, Seven AB, Wetsel AQ, Wetsel WC, Irwin JJ, Skiniotis G, Shoichet BK, Roth BL, Ellman JA
Nature (2022) 610 pp. 582-591 [ Pubmed: 36171289 DOI: doi:10.1038/s41586-022-05258-z ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 5-ht2ar bound to a tetrahydropyridine agonist (r-69) in complex with heterotrimeric mini-gq protein and single-chain variable fragment (scfv16) (Complex from Homo sapiens)
- Single-chain variable fragment 16 (scfv16) (30 kDa, Protein from Homo sapiens)
- G protein subunit q (gi2-mini-gq chimera) (28 kDa, Protein from Homo sapiens)
- 5-hydroxytryptamine receptor 2a (38 kDa, Protein from Homo sapiens)
- (3r)-3-methyl-5-(1h-pyrrolo[2,3-b]pyridin-3-yl)-1,2,3,6-tetrahydropyridin-1-ium (214 Da, Ligand)
CryoEM Structure of Inactive NTSR1 Bound to SR48692 and Nb6
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7ul2
Q-score: 0.645
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- 2-[[1-(7-chloranylquinolin-4-yl)-5-(2,6-dimethoxyphenyl)pyrazol-3-yl]carbonylamino]adamantane-2-carboxylic acid (587 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Ntsr1 (Complex from Homo sapiens)
- Complex of ntsr1 and nb6 (Complex from Homo sapiens)
- Neurotensin receptor 1 (47 kDa, Protein from Homo sapiens)
CryoEM Structure of Inactive H2R Bound to Famotidine, Nb6M, and NabFab
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7ul3
Q-score: 0.36
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nb6m (Complex from Lama glama)
- Nabfab (Complex from synthetic construct)
- H2r (Complex from Homo sapiens)
- Histamine h2 receptor (44 kDa, Protein from Homo sapiens)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nanobody 6m (14 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Complex of h2r, nb6m, and nabfab (Complex from Homo sapiens)
- Famotidine (339 Da, Ligand)
CryoEM Structure of Inactive MOR Bound to Alvimopan and Mb6
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ul4
Q-score: 0.563
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Mb6 (Complex from Lama glama)
- Water (18 Da, Ligand)
- Complex of mor and nb6 (Complex from Mus musculus)
- Mor (Complex from Mus musculus)
- N-[(2s)-2-{[(3r,4r)-4-(3-hydroxyphenyl)-3,4-dimethylpiperidin-1-yl]methyl}-3-phenylpropanoyl]glycine (424 Da, Ligand)
- Mu-type opioid receptor (47 kDa, Protein from Mus musculus)
- Megabody 6 (55 kDa, Protein from synthetic construct)
CryoEM Structure of Inactive SSTR2 bound to Nb6
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ul5
Q-score: 0.562
Robertson MJ, Papasergi-Scott MM, He F, Seven AB, Meyerowitz JG, Panova O, Peroto MC, Che T, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 1188-1195 [ DOI: doi:10.1038/s41594-022-00859-8 Pubmed: 36396979 ]
- Nanobody 6 (14 kDa, Protein from Lama glama)
- Nb6 (Complex from Lama glama)
- Complex of sstr2 and nb6 (Complex from Homo sapiens)
- Somatostatin receptor type 2 (46 kDa, Protein from Homo sapiens)
- Sstr2 (Complex from Homo sapiens)