Cryo-EM structure of Rob-dependent transcription activation complex in a unique conformation
Resolution: 4.57 Å
EM Method: Single-particle
Fitted PDBs: 7vwy
Q-score: 0.359
Shi J, Wang F, Li F, Wang L, Xiong Y, Wen A, Jin Y, Jin S, Gao F, Feng Z, Li J, Zhang Y, Shang Z, Wang S, Feng Y, Lin W
Nucleic Acids Res (2022) 50 pp. 5974-5987 [ DOI: doi:10.1093/nar/gkac433 Pubmed: 35641097 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Right origin-binding protein (33 kDa, Protein from Escherichia coli K-12)
- Rob-dependent transcription activation complex (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Micf promoter dna scaffold forward strand (21 kDa, DNA from Escherichia coli)
- Micf promoter dna scaffold reverse strand (21 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
Cryo-EM structure of Rob-dependent transcription activation complex in a unique conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7vwz
Q-score: 0.345
Shi J, Wang F, Li F, Wang L, Xiong Y, Wen A, Jin Y, Jin S, Gao F, Feng Z, Li J, Zhang Y, Shang Z, Wang S, Feng Y, Lin W
Nucleic Acids Res (2022) 50 pp. 5974-5987 [ DOI: doi:10.1093/nar/gkac433 Pubmed: 35641097 ]
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna (Complex)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli K-12)
- Micf promoter dna reverse strand (21 kDa, DNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Right origin-binding protein (33 kDa, Protein from Escherichia coli K-12)
- Rna polymerase subunits (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Rob-dependent transcription activation complex (Complex from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Micf promoter dna forward strand (21 kDa, DNA from Escherichia coli)
A refined cryo-EM structure of an Escherichia coli RNAP-promoter open complex (RPo) with SspA
Resolution: 3.68 Å
EM Method: Single-particle
Fitted PDBs: 7dy6
Q-score: 0.474
Wang F, Feng Y, Shang Z, Lin W
Biochem Biophys Res Commun (2021) 583 pp. 86-92 [ DOI: doi:10.1016/j.bbrc.2021.10.048 Pubmed: 34735884 ]
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli (strain K12))
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli (strain K12))
- Stringent starvation protein a (24 kDa, Protein from Escherichia coli (strain K12))
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli (strain K12))
- Dna (63-mer) (19 kDa, DNA from Escherichia coli)
- An escherichia coli rnap-promoter open complex (rpo) with sspa (Cell from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Dna (63-mer) (19 kDa, DNA from Escherichia coli)
Subtomogram average of a purified Leptospira biflexa -fcpB mutant flagellum
Gibson KH, Trajtenberg F, Wunder EA, Brady MR, San Martin F, Mechaly A, Shang Z, Liu J, Picardeau M, Ko A, Buschiazzo A, Sindelar CV
eLife (2020) 9 [ Pubmed: 32157997 DOI: doi:10.7554/eLife.53672 ]
Resolution: 9.83 Å
EM Method: Subtomogram averaging
Fitted PDBs: 6pwb
Q-score: 0.059
Gibson KH, Trajtenberg F, Wunder EA, Brady MR, San Martin F, Mechaly A, Shang Z, Liu J, Picardeau M, Ko A, Buschiazzo A, Sindelar CV
eLife (2020) 9 [ Pubmed: 32157997 DOI: doi:10.7554/eLife.53672 ]
- Flagellar filament purified from the periplasm of the spirochete bacterium, leptospira biflexa (Cellular component from Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)')
- Flagellar coiling protein b (fcpb) (25 kDa, Protein from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris))
- Flagellar coiling protein a (fcpa) (28 kDa, Protein from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris))
- Flagellin b1 (flab1) (29 kDa, Protein from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris))
