VGSNKGAIIGL from Amyloid Beta determined by MicroED
Krotee P, Griner SL, Sawaya MR, Cascio D, Rodriguez JA, Shi D, Philipp S, Murray K, Saelices L, Lee J, Seidler P, Glabe CG, Jiang L, Gonen T, Eisenberg DS
J Biol Chem (2018) 293 pp. 2888-2902 [ Pubmed: 29282295 DOI: doi:10.1074/jbc.M117.806109 ]
- Amyloid beta a4 protein (1 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Fibrils of amyloid beta segment 24-34 (Complex from Homo sapiens)
MicroED structure of tau VQIVYK peptide at 1.1 A resolution
Resolution: 1.1 Å
EM Method: Electron Crystallography
Fitted PDBs: 5k7n
Q-score: 0.915
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods (2017) 14 pp. 399-402 [ Pubmed: 28192420 DOI: doi:10.1038/nmeth.4178 ]
- Water (18 Da, Ligand)
- Vqivyk (749 Da, Protein from Homo sapiens)
- Vqivyk (747 Da, Cellular component from Homo sapiens)
MicroED structure of lysozyme at 1.8 A resolution
Resolution: 1.8 Å
EM Method: Electron Crystallography
Fitted PDBs: 5k7o
Q-score: 0.843
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods (2017) 14 pp. 399-402 [ Pubmed: 28192420 DOI: doi:10.1038/nmeth.4178 ]
- Water (18 Da, Ligand)
- Lysozyme c (14 kDa, Protein from Gallus gallus)
- Chloride ion (35 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Lysozyme (14 kDa, Cellular component from Gallus gallus)
MicroED structure of xylanase at 2.3 A resolution
Resolution: 2.3 Å
EM Method: Electron Crystallography
Fitted PDBs: 5k7p
Q-score: 0.724
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods (2017) 14 pp. 399-402 [ Pubmed: 28192420 DOI: doi:10.1038/nmeth.4178 ]
- Water (18 Da, Ligand)
- Endo-1,4-beta-xylanase 2 (20 kDa, Protein from Hypocrea jecorina)
- Xylanase (21 kDa, Cellular component from Trichoderma reesei)
- Iodide ion (126 Da, Ligand)
MicroED structure of thaumatin at 2.5 A resolution
Resolution: 2.5 Å
EM Method: Electron Crystallography
Fitted PDBs: 5k7q
Q-score: 0.714
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods (2017) 14 pp. 399-402 [ Pubmed: 28192420 DOI: doi:10.1038/nmeth.4178 ]
- Thaumatin-1 (22 kDa, Protein from Thaumatococcus daniellii)
- Water (18 Da, Ligand)
- Thaumatin (22 kDa, Cellular component from Thaumatococcus daniellii)
MicroED structure of trypsin at 1.7 A resolution
Resolution: 1.7 Å
EM Method: Electron Crystallography
Fitted PDBs: 5k7r
Q-score: 0.855
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods (2017) 14 pp. 399-402 [ Pubmed: 28192420 DOI: doi:10.1038/nmeth.4178 ]
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
- Trypsin (23 kDa, Cellular component from Bos taurus)
- Cationic trypsin (23 kDa, Protein from Bos taurus)
MicroED structure of proteinase K at 1.6 A resolution
Resolution: 1.6 Å
EM Method: Electron Crystallography
Fitted PDBs: 5k7s
Q-score: 0.891
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods (2017) 14 pp. 399-402 [ Pubmed: 28192420 DOI: doi:10.1038/nmeth.4178 ]
- Calcium ion (40 Da, Ligand)
- Water (18 Da, Ligand)
- Proteinase k (28 kDa, Cellular component from Engyodontium album)
- Proteinase k (28 kDa, Protein from Engyodontium album)
MicroED structure of thermolysin at 2.5 A resolution
Resolution: 2.5 Å
EM Method: Electron Crystallography
Fitted PDBs: 5k7t
Q-score: 0.719
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods (2017) 14 pp. 399-402 [ Pubmed: 28192420 DOI: doi:10.1038/nmeth.4178 ]
- Thermolysin (34 kDa, Cellular component from Bacillus thermoproteolyticus)
- Water (18 Da, Ligand)
- Isopropyl alcohol (60 Da, Ligand)
- Thermolysin (34 kDa, Protein from Bacillus thermoproteolyticus)
- Dimethyl sulfoxide (78 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)