Cryo-EM structure of RCMV-E E27 bound to human DDB1 (deltaBPB) and rat STAT2 CCD
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7zn7
Q-score: 0.395
Le-Trilling VTK, Banchenko S, Paydar D, Leipe PM, Binting L, Lauer S, Graziadei A, Klingen R, Gotthold C, Burger J, Bracht T, Sitek B, Jan Lebbink R, Malyshkina A, Mielke T, Rappsilber J, Spahn CM, Voigt S, Trilling M, Schwefel D
EMBO J (2023) 42 pp. e112351-e112351 [ Pubmed: 36762436 DOI: doi:10.15252/embj.2022112351 ]
- Dna damage-binding protein 1 (93 kDa, Protein from Homo sapiens)
- Ternary complex of rcmv-e e27 with human ddb1 (deltabpb) and rat stat2 (100 kDa, Complex from Murid betaherpesvirus 8)
- E27 (Complex from Murid betaherpesvirus 8)
- Signal transducer and activator of transcription (97 kDa, Protein from Rattus norvegicus)
- Zinc ion (65 Da, Ligand)
- B27a (76 kDa, Protein from Murid betaherpesvirus 8)
- Dna damage-binding protein 1 (Complex from Homo sapiens)
- Signal transducer and activator of transcription (Complex from Rattus norvegicus)
Cryo-EM structure of RCMV-E E27 bound to human DDB1 (deltaBPB) and full-length rat STAT2
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7znn
Q-score: 0.139
Le-Trilling VTK, Banchenko S, Paydar D, Leipe PM, Binting L, Lauer S, Graziadei A, Klingen R, Gotthold C, Burger J, Bracht T, Sitek B, Jan Lebbink R, Malyshkina A, Mielke T, Rappsilber J, Spahn CM, Voigt S, Trilling M, Schwefel D
EMBO J (2023) 42 pp. e112351-e112351 [ Pubmed: 36762436 DOI: doi:10.15252/embj.2022112351 ]
- Dna damage-binding protein 1 (93 kDa, Protein from Homo sapiens)
- Ternary complex of rcmv-e e27 with human ddb1 (deltabpb) and rat stat2 (100 kDa, Complex from Murid betaherpesvirus 8)
- E27 (Complex from Murid betaherpesvirus 8)
- Signal transducer and activator of transcription (97 kDa, Protein from Rattus norvegicus)
- Zinc ion (65 Da, Ligand)
- B27a (76 kDa, Protein from Murid betaherpesvirus 8)
- Dna damage-binding protein 1 (Complex from Homo sapiens)
- Signal transducer and activator of transcription (Complex from Rattus norvegicus)
Nonstop ribosomal complex bound with Dom34 and Hbs1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 5m1j
Q-score: 0.45
Hilal T, Yamamoto H, Loerke J, Burger J, Mielke T, Spahn CM
Nat Commun (2016) 7 pp. 13521-13521 [ DOI: doi:10.1038/ncomms13521 Pubmed: 27995908 ]
- 40s ribosomal protein s28-b (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s20 (12 kDa, Protein from Saccharomyces cerevisiae)
- Yeast phe-trna-phe (24 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae)
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae)
- N-acetyl-l-phenylalanine (207 Da, Ligand)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l34-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l8-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- Protein hbs1 (68 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s25-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- 60s ribosomal protein l7-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l6-a (19 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-b (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (10 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Protein dom34 (43 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Ubiquitin-40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s15 (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s5 (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- 40s ribosomal protein s14-a (13 kDa, Protein from Saccharomyces cerevisiae)
- Nonstop mrna (6 kDa, RNA from synthetic construct)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-b (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-b (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l24-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l41-b (3 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Nonstop ribosomal complex bound with dom34-hbs1-gmppnp (3 MDa, Complex)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 4d5n, 4d5l, 4d5y
Q-score: 0.10566667
Muhs M, Hilal T, Mielke T, Skabkin MA, Sanbonmatsu KY, Pestova TV, Spahn CM
Mol Cell (2015) 57 pp. 422-432 [ Pubmed: 25601755 DOI: doi:10.1016/j.molcel.2014.12.016 ]
- Eukaryoric release factor 1 (50 kDa, Protein from Homo sapiens)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Ribosomal 80s termination complex with crpv ires-rna and erf1 (4 MDa, Sample)
- Cricket paralysis virus ires rna (RNA from Cricket paralysis virus)
Resolution: 8.9 Å
EM Method: Single-particle
Fitted PDBs: 4d61, 4d67
Q-score: 0.1345
Muhs M, Hilal T, Mielke T, Skabkin MA, Sanbonmatsu KY, Pestova TV, Spahn CM
Mol Cell (2015) 57 pp. 422-432 [ Pubmed: 25601755 DOI: doi:10.1016/j.molcel.2014.12.016 ]
- Ribosomal 80s termination complex with crpv ires-rna, erf1 and erf3 (4 MDa, Sample)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Eukaryotic release factor 3 (68 kDa, Protein from Homo sapiens)
- Cricket paralysis virus ires rna (RNA from Cricket paralysis virus)
- Eukaryoric release factor 1 (50 kDa, Protein from Homo sapiens)
Cryo-electron microscopy of human 80S ribosome
Penczek PA, Fang J, Li X, Cheng Y, Loerke J, Spahn CM
Ultramicroscopy (2014) 140 pp. 9-19 [ Pubmed: 24562077 DOI: doi:10.1016/j.ultramic.2014.01.009 ]
- 80s ribosome (4 MDa, Complex from Homo sapiens)
- Human 80s ribosome (4 MDa, Sample)
Cryo-EM reconstruction of a authentic 70S-tRNA2-mRNA-EF-G translocation intermediate
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 4v7b
Q-score: 0.161
Ramrath DJ, Lancaster L, Sprink T, Mielke T, Loerke J, Noller HF, Spahn CM
PNAS (2013) 110 pp. 20964-20969 [ Pubmed: 24324168 DOI: doi:10.1073/pnas.1320387110 ]
- Formyl-methionine specific initiator transfer rna (RNA from Escherichia coli)
- Ribosomal 70s-ef-g complex at an authentic intermediate state during translocation (3 MDa, Sample)
- Elongation factor g (Protein from Escherichia coli)
- Modified formyl-methionine specific initiator transfer rna (RNA from Escherichia coli)
- 70s ribosome (3 MDa, Complex from Escherichia coli)
- Messenger rna (RNA from Escherichia coli)
tmRNA translocation and MLD-loading on the ribosome: a 70S-tmRNA-EF-G complex
Resolution: 8.3 Å
EM Method: Single-particle
Fitted PDBs: 4v6t
Q-score: 0.136
Ramrath DJ, Yamamoto H, Rother K, Wittek D, Pech M, Mielke T, Loerke J, Scheerer P, Ivanov P, Teraoka Y, Shpanchenko O, Nierhaus KH, Spahn CM
Nature (2012) 485 pp. 526-529 [ Pubmed: 22622583 DOI: doi:10.1038/nature11006 ]
- Ribosome (3 MDa, Complex from Escherichia coli)
- Complex of 70s-tmrna-smpb-ef-g (3 MDa, Sample)
Cryo EM reconstruction of mammalian 80S ribosome in classic PRE state 1
Resolution: 9.8 Å
EM Method: Single-particle
Fitted PDBs: 3j0l
Q-score: 0.104
Budkevich T, Giesebrecht J, Altman RB, Munro JB, Mielke T, Nierhaus KH, Blanchard SC, Spahn CM
Mol Cell (2011) 44 pp. 214-224 [ Pubmed: 22017870 DOI: doi:10.1016/j.molcel.2011.07.040 ]
Cryo EM reconstruction of mammalian 80S ribosome in classic PRE state 2
Resolution: 9.0 Å
EM Method: Single-particle
Fitted PDBs: 3j0o
Q-score: 0.119
Budkevich T, Giesebrecht J, Altman RB, Munro JB, Mielke T, Nierhaus KH, Blanchard SC, Spahn CM
Mol Cell (2011) 44 pp. 214-224 [ Pubmed: 22017870 DOI: doi:10.1016/j.molcel.2011.07.040 ]