Wiechert F, Unbehaun A, Sprink T, Seibel H, Burger J, Loerke J, Mielke T, Diebolder CA, Schacherl M, Spahn CMT
Nucleic Acids Res (2025) 53 [ Pubmed: 39658079 DOI: doi:10.1093/nar/gkae1191 ]
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- Spermine (202 Da, Ligand)
- Large ribosomal subunit protein el40 (14 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul2 (28 kDa, Protein from Homo sapiens)
- Spermidine (145 Da, Ligand)
- Large ribosomal subunit protein ul30 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 1,4-diaminobutane (88 Da, Ligand)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- Human mature large ribosomal subunit (Complex from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- Large ribosomal subunit protein ul3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
Cryo-electron tomography structure of membrane-bound EHD4 complex
Resolution: 7.6 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7sox
Q-score: 0.086
Melo AA, Sprink T, Noel JK, Vazquez-Sarandeses E, van Hoorn C, Mohd S, Loerke J, Spahn CMT, Daumke O
Nat Commun (2022) 13 pp. 7641-7641 [ DOI: doi:10.1038/s41467-022-35164-x Pubmed: 36496453 ]
- Ehd4 with amppnp and liposomes (590 kDa, Cellular component from Mus musculus)
- Eh domain-containing protein 4 (58 kDa, Protein from Mus musculus)
Cryo-electron microscopy structure of ribosome-bound initiation factor 2
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 3jcn
Q-score: 0.241
Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke J, Ismer J, Hildebrand PW, Scheerer P, Buerger J, Mielke T, Spahn CMT
Sci Adv (2016)
- Initiation factor 2 was stalled on escherichia coli 70s ribosomes by the non-hydrolysable gtp analogue gdpnp in the presence of fmet-trnaimet and mrna. (2 MDa, Sample)
- Initiation factor 2 (97 kDa, Protein from Escherichia coli)
- Fmet-trnaimet (RNA from Escherichia coli)
- 70s ribosome (2 MDa, Complex from Escherichia coli)
Cryo-electron microscopy structure of ribosome-bound initiation factor 2 70S IC II state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 3jcj
Q-score: 0.35
Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke J, Ismer J, Hildebrand PW, Scheerer P, Buerger J, Mielke T, Spahn CMT
Sci Adv (2016) 2 pp. e1501502-e1501502
- Fmet-trnaimet (RNA from Escherichia coli)
- Escherichia coli initiation factor 2 stalled on escherichia coli 70s ribosomes by the non-hydrolysable gtp analogue gdpnp in the presence of fmet-trnaimet and mrna (2 MDa, Sample)
- Initiation factor 2 (97 kDa, Protein from Escherichia coli)
- 70s ribosome (2 MDa, Complex from Escherichia coli)
Mammalian 80S HCV-IRES complex, Classical
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5flx
Q-score: 0.261
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s-hcv-ires complex, classical (4 MDa, Sample)
Mammalian 80S HCV-IRES complex, Classical / no head tilt
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s hcv-ires complex, classical / no head tilt (4 MDa, Sample)
- Trna (2 kDa, RNA from Oryctolagus cuniculus)
Mammalian 40S HCV-IRES complex
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Mammalian 40s-hcv-ires complex (1 MDa, Sample)
- 40s ribosome (1 MDa, Complex from Oryctolagus cuniculus)
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
Mammalian 80S HCV-IRES complex, Rolled
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s hcv-ires complex, rotated (4 MDa, Sample)
Mammalian 80S HCV-IRES complex, Rotated
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s hcv-ires complex, rotated (4 MDa, Sample)
Cryo-EM reconstruction of a authentic 70S-tRNA2-mRNA-EF-G translocation intermediate
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 4v7b
Q-score: 0.161
Ramrath DJ, Lancaster L, Sprink T, Mielke T, Loerke J, Noller HF, Spahn CM
PNAS (2013) 110 pp. 20964-20969 [ DOI: doi:10.1073/pnas.1320387110 Pubmed: 24324168 ]
- Formyl-methionine specific initiator transfer rna (RNA from Escherichia coli)
- Elongation factor g (Protein from Escherichia coli)
- Messenger rna (RNA from Escherichia coli)
- Ribosomal 70s-ef-g complex at an authentic intermediate state during translocation (3 MDa, Sample)
- Modified formyl-methionine specific initiator transfer rna (RNA from Escherichia coli)
- 70s ribosome (3 MDa, Complex from Escherichia coli)