Cryo-electron microscopy structure of ribosome-bound initiation factor 2
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 3jcn
Q-score: 0.241
Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke J, Ismer J, Hildebrand PW, Scheerer P, Buerger J, Mielke T, Spahn CMT
Sci Adv (2016)
- Initiation factor 2 was stalled on escherichia coli 70s ribosomes by the non-hydrolysable gtp analogue gdpnp in the presence of fmet-trnaimet and mrna. (2 MDa, Sample)
- Initiation factor 2 (97 kDa, Protein from Escherichia coli)
- Fmet-trnaimet (RNA from Escherichia coli)
- 70s ribosome (2 MDa, Complex from Escherichia coli)
Cryo-electron microscopy structure of ribosome-bound initiation factor 2 70S IC II state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 3jcj
Q-score: 0.35
Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke J, Ismer J, Hildebrand PW, Scheerer P, Buerger J, Mielke T, Spahn CMT
Sci Adv (2016) 2 pp. e1501502-e1501502
- Fmet-trnaimet (RNA from Escherichia coli)
- Escherichia coli initiation factor 2 stalled on escherichia coli 70s ribosomes by the non-hydrolysable gtp analogue gdpnp in the presence of fmet-trnaimet and mrna (2 MDa, Sample)
- Initiation factor 2 (97 kDa, Protein from Escherichia coli)
- 70s ribosome (2 MDa, Complex from Escherichia coli)
Mammalian 80S HCV-IRES complex, Classical
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5flx
Q-score: 0.261
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s-hcv-ires complex, classical (4 MDa, Sample)
Mammalian 80S HCV-IRES complex, Classical / no head tilt
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s hcv-ires complex, classical / no head tilt (4 MDa, Sample)
- Trna (2 kDa, RNA from Oryctolagus cuniculus)
Mammalian 40S HCV-IRES complex
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Mammalian 40s-hcv-ires complex (1 MDa, Sample)
- 40s ribosome (1 MDa, Complex from Oryctolagus cuniculus)
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
Mammalian 80S HCV-IRES complex, Rolled
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s hcv-ires complex, rotated (4 MDa, Sample)
Mammalian 80S HCV-IRES complex, Rotated
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s hcv-ires complex, rotated (4 MDa, Sample)
Cryo-EM reconstruction of a authentic 70S-tRNA2-mRNA-EF-G translocation intermediate
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 4v7b
Q-score: 0.161
Ramrath DJ, Lancaster L, Sprink T, Mielke T, Loerke J, Noller HF, Spahn CM
PNAS (2013) 110 pp. 20964-20969 [ DOI: doi:10.1073/pnas.1320387110 Pubmed: 24324168 ]
- Formyl-methionine specific initiator transfer rna (RNA from Escherichia coli)
- Elongation factor g (Protein from Escherichia coli)
- Messenger rna (RNA from Escherichia coli)
- Ribosomal 70s-ef-g complex at an authentic intermediate state during translocation (3 MDa, Sample)
- Modified formyl-methionine specific initiator transfer rna (RNA from Escherichia coli)
- 70s ribosome (3 MDa, Complex from Escherichia coli)