Map of the 80S Mouse ribosome with A- and P-site tRNA
Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Gunnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Munster-Wandowski A, Burger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT
Mol Cell (2021) 81 pp. 304-322.e16 [ Pubmed: 33357414 DOI: doi:10.1016/j.molcel.2020.11.037 ]
Map of the 80S Mouse ribosome with eEF2 and EBP1.
Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Gunnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Munster-Wandowski A, Burger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT
Mol Cell (2021) 81 pp. 304-322.e16 [ Pubmed: 33357414 DOI: doi:10.1016/j.molcel.2020.11.037 ]
Map of the 80S Mouse ribosome with A- and P-site tRNA
Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Gunnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Munster-Wandowski A, Burger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT
Mol Cell (2021) 81 pp. 304-322.e16 [ DOI: doi:10.1016/j.molcel.2020.11.037 Pubmed: 33357414 ]
Map of the 80S Mouse ribosome with eEF2.
Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Gunnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Munster-Wandowski A, Burger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT
Mol Cell (2021) 81 pp. 304-322.e16 [ Pubmed: 33357414 DOI: doi:10.1016/j.molcel.2020.11.037 ]
Mus musculus brain neocortex ribosome 60S bound to Ebp1
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6swa
Q-score: 0.449
Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Gunnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Munster-Wandowski A, Burger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT
Mol Cell (2021) 81 pp. 304- [ Pubmed: 33357414 DOI: doi:10.1016/j.molcel.2020.11.037 ]
- 60s ribosomal protein l13a (22 kDa, Protein from Mus musculus)
- 60s ribosomal protein l23a (13 kDa, Protein from Mus musculus)
- 60s ribosomal protein l36 (11 kDa, Protein from Mus musculus)
- Ribosomal protein l39 (6 kDa, Protein from Mus musculus)
- 60s ribosomal protein l24 (7 kDa, Protein from Mus musculus)
- 60s ribosomal protein l38 (8 kDa, Protein from Mus musculus)
- 60s ribosomal protein l14 (16 kDa, Protein from Mus musculus)
- 60s ribosomal protein l30 (10 kDa, Protein from Mus musculus)
- 28s ribosomal rna (1 MDa, RNA from Mus musculus)
- 60s ribosomal protein l27 (15 kDa, Protein from Mus musculus)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l41 (3 kDa, Protein from Mus musculus)
- 60s ribosomal protein l34 (12 kDa, Protein from Mus musculus)
- 60s ribosomal protein l35a (12 kDa, Protein from Mus musculus)
- 60s ribosomal protein l8 (27 kDa, Protein from Mus musculus)
- Ribosomal protein l26 (15 kDa, Protein from Mus musculus)
- Ribosomal protein l37a (10 kDa, Protein from Mus musculus)
- 60s ribosomal protein l18a (20 kDa, Protein from Mus musculus)
- 60s ribosomal protein l5 (34 kDa, Protein from Mus musculus)
- 60s ribosomal protein l7 (27 kDa, Protein from Mus musculus)
- 60s ribosomal protein l23 (13 kDa, Protein from Mus musculus)
- 60s ribosomal protein l37 (9 kDa, Protein from Mus musculus)
- Proliferation-associated protein 2g4 (39 kDa, Protein from Mus musculus)
- 60s ribosomal protein l15 (24 kDa, Protein from Mus musculus)
- 60s ribosomal protein l3 (45 kDa, Protein from Mus musculus)
- 60s ribosomal protein l7a (26 kDa, Protein from Mus musculus)
- 60s ribosomal protein l22 (11 kDa, Protein from Mus musculus)
- 60s ribosomal protein l19 (22 kDa, Protein from Mus musculus)
- 60s ribosomal protein l21 (18 kDa, Protein from Mus musculus)
- Mus musculus postnatal day 0 brain neocortex 80s ribosome bound to ebp1 (3 MDa, Complex from Mus musculus)
- 60s ribosomal protein l9 (21 kDa, Protein from Mus musculus)
- 60s ribosomal protein l17 (17 kDa, Protein from Mus musculus)
- 5s ribosomal rna (38 kDa, RNA from Mus musculus)
- 60s ribosomal protein l11 (19 kDa, Protein from Mus musculus)
- 60s ribosomal protein l4 (41 kDa, Protein from Mus musculus)
- 60s ribosomal protein l18 (21 kDa, Protein from Mus musculus)
- 60s ribosomal protein l10 (24 kDa, Protein from Mus musculus)
- 60s ribosomal protein l35 (14 kDa, Protein from Mus musculus)
- 60s ribosomal protein l40 (5 kDa, Protein from Mus musculus)
- 60s ribosomal protein l28 (14 kDa, Protein from Mus musculus)
- 60s ribosomal protein l32 (15 kDa, Protein from Mus musculus)
- 60s ribosomal protein l29 (7 kDa, Protein from Mus musculus)
- 60s ribosomal protein l31 (12 kDa, Protein from Mus musculus)
- 60s ribosomal protein l27a (16 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- 5.8s ribosomal rna (50 kDa, RNA from Mus musculus)
- 60s ribosomal protein l6 (22 kDa, Protein from Mus musculus)
- Ribosomal protein l36a (12 kDa, Protein from Mus musculus)
- 60s ribosomal protein l13 (23 kDa, Protein from Mus musculus)
Cryo-electron microscopy structure of ribosome-bound initiation factor 2
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 3jcn
Q-score: 0.241
Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke J, Ismer J, Hildebrand PW, Scheerer P, Buerger J, Mielke T, Spahn CMT
Sci Adv (2016)
- Initiation factor 2 was stalled on escherichia coli 70s ribosomes by the non-hydrolysable gtp analogue gdpnp in the presence of fmet-trnaimet and mrna. (2 MDa, Sample)
- Initiation factor 2 (97 kDa, Protein from Escherichia coli)
- Fmet-trnaimet (RNA from Escherichia coli)
- 70s ribosome (2 MDa, Complex from Escherichia coli)
Cryo-electron microscopy structure of ribosome-bound initiation factor 2 70S IC II state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 3jcj
Q-score: 0.35
Sprink T, Ramrath DJF, Yamamoto H, Yamamoto K, Loerke J, Ismer J, Hildebrand PW, Scheerer P, Buerger J, Mielke T, Spahn CMT
Sci Adv (2016) 2 pp. e1501502-e1501502
- Fmet-trnaimet (RNA from Escherichia coli)
- Escherichia coli initiation factor 2 stalled on escherichia coli 70s ribosomes by the non-hydrolysable gtp analogue gdpnp in the presence of fmet-trnaimet and mrna (2 MDa, Sample)
- Initiation factor 2 (97 kDa, Protein from Escherichia coli)
- 70s ribosome (2 MDa, Complex from Escherichia coli)
Mammalian 80S HCV-IRES complex, Classical
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5flx
Q-score: 0.261
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s-hcv-ires complex, classical (4 MDa, Sample)
Mammalian 80S HCV-IRES complex, Classical / no head tilt
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)
- 80s ribosome (4 MDa, Complex from Oryctolagus cuniculus)
- Mammalian 80s hcv-ires complex, classical / no head tilt (4 MDa, Sample)
- Trna (2 kDa, RNA from Oryctolagus cuniculus)
Mammalian 40S HCV-IRES complex
Yamamoto H, Collier M, Loerke J, Ismer J, Schmidt A, Hilal T, Sprink T, Yamamoto K, Mielke T, Burger J, Shaikh TR, Dabrowski M, Hildebrand PW, Scheerer P, Spahn CMT
EMBO J (2015) 34 pp. 3042-3058 [ Pubmed: 26604301 DOI: doi:10.15252/embj.201592469 ]
- Mammalian 40s-hcv-ires complex (1 MDa, Sample)
- 40s ribosome (1 MDa, Complex from Oryctolagus cuniculus)
- Hcv-ires (162 kDa, RNA from Hepatitis C virus)