Structure of Setaria italica NRAT in complex with a nanobody
Resolution: 3.66 Å
EM Method: Single-particle
Fitted PDBs: 8ont
Q-score: 0.467
Ramanadane K, Liziczai M, Markovic D, Straub MS, Rosalen GT, Udovcic A, Dutzler R, Manatschal C
eLife (2023) 12 [ DOI: doi:10.7554/eLife.85641 Pubmed: 37074929 ]
- Complex between sinrat and a nanobody used as a fiducial marker for structure determination (Complex from Setaria italica)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Nanobody1 (13 kDa, Protein from Lama glama)
- Nramp related aluminium transporter (60 kDa, Protein from Setaria italica)
- Water (18 Da, Ligand)
Structure of mTMEM16F in lipid Nanodiscs in the presence of Ca2+
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8b8q
Q-score: 0.453
Arndt M, Alvadia C, Straub MS, Clerico Mosina V, Paulino C, Dutzler R
Nat Commun (2022) 13 pp. 6692-6692 [ DOI: doi:10.1038/s41467-022-34497-x Pubmed: 36335104 ]
- Mtmem16f homodimer bound to ca2+ (Complex from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Anoctamin-6 (113 kDa, Protein from Mus musculus)
Cryo-EM structure of Ca2+-bound mTMEM16F F518H mutant in Digitonin
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8b8j
Q-score: 0.459
Arndt M, Alvadia C, Straub MS, Clerico Mosina V, Paulino C, Dutzler R
Nat Commun (2022) 13 pp. 6692-6692 [ DOI: doi:10.1038/s41467-022-34497-x Pubmed: 36335104 ]
- Mtmem16f ca2+ bound (Complex from Mus musculus)
- Anoctamin-6 (113 kDa, Protein from Mus musculus)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of Ca2+-bound mTMEM16F N562A mutant in Digitonin closed/closed
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 8b8k
Q-score: 0.501
Arndt M, Alvadia C, Straub MS, Clerico Mosina V, Paulino C, Dutzler R
Nat Commun (2022) 13 pp. 6692-6692 [ DOI: doi:10.1038/s41467-022-34497-x Pubmed: 36335104 ]
- Anoctamin-6 (113 kDa, Protein from Mus musculus)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Mtmem16f ca2+-bound (Complex from Mus musculus)
Cryo-EM structure of Ca2+-bound mTMEM16F N562A mutant in Digitonin open/closed
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8b8m
Q-score: 0.391
Arndt M, Alvadia C, Straub MS, Clerico Mosina V, Paulino C, Dutzler R
Nat Commun (2022) 13 pp. 6692-6692 [ DOI: doi:10.1038/s41467-022-34497-x Pubmed: 36335104 ]
- Mtmem16f n562a mutant (Complex from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Anoctamin-6 (113 kDa, Protein from Mus musculus)
Cryo-EM structure of Ca2+-bound mTMEM16F F518A Q623A mutant in GDN open/closed
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8bc0
Q-score: 0.34
Arndt M, Alvadia C, Straub MS, Clerico Mosina V, Paulino C, Dutzler R
Nat Commun (2022) 13 pp. 6692-6692 [ DOI: doi:10.1038/s41467-022-34497-x Pubmed: 36335104 ]
- Mtmem16f f518a q623a mutant (Complex from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Anoctamin-6 (113 kDa, Protein from Mus musculus)
Cryo-EM Structure of Ca2+-bound mTMEM16F F518A_Q623A mutant in GDN
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8bc1
Q-score: 0.295
Arndt M, Alvadia C, Straub MS, Clerico Mosina V, Paulino C, Dutzler R
Nat Commun (2022) 13 pp. 6692-6692 [ DOI: doi:10.1038/s41467-022-34497-x Pubmed: 36335104 ]
- Homodimeric mtmem16f f518a q623a mutant bound to ca2+ (Complex from Mus musculus)
- Calcium ion (40 Da, Ligand)
- Anoctamin-6,mtmem16f (113 kDa, Protein from Mus musculus)
Cryo-EM map of apo-EleNRMT in complex with two nanobodies at 3.5A
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7qia
Q-score: 0.491
Ramanadane K, Straub MS, Dutzler R, Manatschal C
eLife (2022) 11 [ DOI: doi:10.7554/eLife.74589 Pubmed: 35001872 ]
- Divalent metal cation transporter (46 kDa, Protein from Eggerthella lenta)
- Nanobody 2 (13 kDa, Protein from synthetic construct)
- Nanobody 2 (Cellular component from synthetic construct)
- Elenrmt (Cellular component from Eggerthella lenta)
- Nanobody 1 (13 kDa, Protein from synthetic construct)
- Nanobody 1 (Cellular component from synthetic construct)
- Elenrmt in complex with two nanobodies (13 kDa, Complex)
- Water (18 Da, Ligand)
Cryo-EM map of magnesium-bound EleNRMT in complex with two nanobodies at 4.1A
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7qic
Q-score: 0.416
Ramanadane K, Straub MS, Dutzler R, Manatschal C
eLife (2022) 11 [ DOI: doi:10.7554/eLife.74589 Pubmed: 35001872 ]
- Divalent metal cation transporter (46 kDa, Protein from Eggerthella lenta)
- Magnesium-bound elenrmt in complex with two nanobodies (13 kDa, Complex)
- Nanobody 2 (13 kDa, Protein from synthetic construct)
- Nanobody 2 (Cellular component from synthetic construct)
- Magnesium-bound elenrmt (Cellular component from Eggerthella lenta)
- Nanobody 1 (13 kDa, Protein from synthetic construct)
- Nanobody 1 (Cellular component from synthetic construct)
- Magnesium ion (24 Da, Ligand)
Cryo-EM density of full-length rXKR9 in complex with a sybody at 3.66A
Resolution: 3.66 Å
EM Method: Single-particle
Fitted PDBs: 7p14
Q-score: 0.457
Straub MS, Alvadia C, Sawicka M, Dutzler R
eLife (2021) 10 [ DOI: doi:10.7554/eLife.69800 Pubmed: 34263724 ]
- Xk-related protein (43 kDa, Protein from Rattus norvegicus)
- Rxkr9 (Complex from Rattus norvegicus)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Sybody (Complex from synthetic construct)
- Rxkr9 with sybody (17 kDa, Complex from Rattus norvegicus)
- Sybody (13 kDa, Protein from synthetic construct)