CryoEM structure of Gi-coupled MRGPRX1 with peptide agonist CNF-Tx2
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8jgb
Q-score: 0.551
Guo L, Zhang Y, Fang G, Tie L, Zhuang Y, Xue C, Liu Q, Zhang M, Zhu K, You C, Xu P, Yuan Q, Zhang C, Liu L, Rong N, Peng S, Liu Y, Wang C, Luo X, Lv Z, Kang D, Yu X, Zhang C, Jiang Y, Dong X, Zhou J, Liu Z, Yang F, Eric Xu H, Sun JP
Nat Commun (2023) 14 pp. 5004-5004 [ Pubmed: 37591889 DOI: doi:10.1038/s41467-023-40705-z ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (35 kDa, Protein from Homo sapiens)
- Cryo-em structure of the bam8-22-bound mrgprx1-gq complex (Complex from Homo sapiens)
- Conorfamide-tx2 (2 kDa, Protein from Conus textile)
CryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8jgf
Q-score: 0.486
Guo L, Zhang Y, Fang G, Tie L, Zhuang Y, Xue C, Liu Q, Zhang M, Zhu K, You C, Xu P, Yuan Q, Zhang C, Liu L, Rong N, Peng S, Liu Y, Wang C, Luo X, Lv Z, Kang D, Yu X, Zhang C, Jiang Y, Dong X, Zhou J, Liu Z, Yang F, Eric Xu H, Sun JP
Nat Commun (2023) 14 pp. 5004-5004 [ Pubmed: 37591889 DOI: doi:10.1038/s41467-023-40705-z ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (35 kDa, Protein from Homo sapiens)
- Bam8-22 (1 kDa, Protein from Homo sapiens)
- Cryo-em structure of the bam8-22-bound mrgprx1-gq complex (Complex from Homo sapiens)
- Single-chain fragment variable 16 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein gq (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
CryoEM structure of Gi-coupled MRGPRX1 with peptide agonist BAM8-22
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8jgg
Q-score: 0.54
Guo L, Zhang Y, Fang G, Tie L, Zhuang Y, Xue C, Liu Q, Zhang M, Zhu K, You C, Xu P, Yuan Q, Zhang C, Liu L, Rong N, Peng S, Liu Y, Wang C, Luo X, Lv Z, Kang D, Yu X, Zhang C, Jiang Y, Dong X, Zhou J, Liu Z, Yang F, Eric Xu H, Sun JP
Nat Commun (2023) 14 pp. 5004-5004 [ Pubmed: 37591889 DOI: doi:10.1038/s41467-023-40705-z ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Single-chain fragment variable 16 (30 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Cryoem structure of gi-coupled mrgprx1 with peptide agonist bam8-22 (Complex from Homo sapiens)
- Bam8-22 (1 kDa, Protein from Homo sapiens)
Cryo-EM structure of the P395-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfm
Q-score: 0.517
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ DOI: doi:10.1038/s41586-020-2569-1 Pubmed: 32698187 ]
- 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5h-pyrido[4,3-d]pyrimidine-4-carboxamide (395 Da, Ligand)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- P395-bound gpbar-gs complex (Complex)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- P395 (Complex from synthetic construct)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpbar (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)
Cryo-EM structure of the INT-777-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfn
Q-score: 0.53
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ DOI: doi:10.1038/s41586-020-2569-1 Pubmed: 32698187 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein subunits (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Int-777-bound gpbar-gs complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- G-protein coupled bile acid receptor 1 (Complex)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from Homo sapiens)
- (2s,4r)-4-[(3r,5s,6r,7r,8r,9s,10s,12s,13r,14s,17r)-6-ethyl-10,13-dimethyl-3,7,12-tris(oxidanyl)-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methyl-pentanoic acid (450 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)