T1AM-bound hTAAR1-Gs protein complex
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 8jln
Q-score: 0.427
Xu Z, Guo L, Yu J, Shen S, Wu C, Zhang W, Zhao C, Deng Y, Tian X, Feng Y, Hou H, Su L, Wang H, Guo S, Wang H, Wang K, Chen P, Zhao J, Zhang X, Yong X, Cheng L, Liu L, Yang S, Yang F, Wang X, Yu X, Xu Y, Sun JP, Yan W, Shao Z
Nature (2023) 624 pp. 672-681 [ DOI: doi:10.1038/s41586-023-06804-z Pubmed: 37935376 ]
- Gs heterotrimer (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,trace amine-associated receptor 1 (54 kDa, Protein from Homo sapiens)
- Htaar1-bound t1am in complex with gs heterotrimer (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Mus musculus)
- T1am-bound htaar1 (Complex from Homo sapiens)
- 4-[4-(2-azanylethyl)-2-iodanyl-phenoxy]phenol (355 Da, Ligand)
- Scfv16 (Complex from Mus musculus)
- Mgs (43 kDa, Protein from Homo sapiens)
A77636-bound hTAAR1-Gs protein complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8jlr
Q-score: 0.462
Xu Z, Guo L, Yu J, Shen S, Wu C, Zhang W, Zhao C, Deng Y, Tian X, Feng Y, Hou H, Su L, Wang H, Guo S, Wang H, Wang K, Chen P, Zhao J, Zhang X, Yong X, Cheng L, Liu L, Yang S, Yang F, Wang X, Yu X, Xu Y, Sun JP, Yan W, Shao Z
Nature (2023) 624 pp. 672-681 [ DOI: doi:10.1038/s41586-023-06804-z Pubmed: 37935376 ]
- Gs heterotrimer (Complex from Homo sapiens)
- Soluble cytochrome b562,trace amine-associated receptor 1 (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- (1~{s},3~{r})-3-(1-adamantyl)-1-(aminomethyl)-3,4-dihydro-1~{h}-isochromene-5,6-diol (329 Da, Ligand)
- Scfv16 (Complex from Mus musculus)
- Mgs (41 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- A7736-bound htaar1 (Complex from Homo sapiens)
- Htaar1-bound a77636 in complex with gs heterotrimer (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of the R399-bound GPBAR-Gs complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7xtq
Q-score: 0.456
Ma L, Yang F, Wu X, Mao C, Guo L, Miao T, Zang SK, Jiang X, Shen DD, Wei T, Zhou H, Wei Q, Li S, Shu Q, Feng S, Jiang C, Chu B, Du L, Sun JP, Yu X, Zhang Y, Zhang P
PNAS (2022) 119 pp. e2117054119-e2117054119 [ DOI: doi:10.1073/pnas.2117054119 Pubmed: 35858343 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- R399-bound gpbar-gs complex (Complex)
- [2-(2,5-dichloro-phenoxy)-pyridin-3-yl]-(3,4-dihydro-2h-quinolin-1-yl)-methanone (399 Da, Ligand)
- Nanobody-35 (Complex from synthetic construct)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpbar (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)
Cryo-EM structure of a-MSH-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f53
Q-score: 0.474
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (1 kDa, Protein from synthetic construct)
- Complex of alpha-msh bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Alpha-melanocyte-stimulating hormones (Complex)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of afamelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f54
Q-score: 0.408
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Complex of afamelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Afamelanotide (Complex)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Calcium ion (40 Da, Ligand)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of bremelanotide-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f55
Q-score: 0.411
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Bremelanotide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Complex of bremelanotide bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Melanocrotin-4 receptor (Complex)
- Bremelanotide (1 kDa, Protein from synthetic construct)
- Nanobody35 (Complex from Homo sapiens)
Cryo-EM structure of THIQ-MC4R-Gs_Nb35 complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7f58
Q-score: 0.516
Zhang H, Chen LN, Yang D, Mao C, Shen Q, Feng W, Shen DD, Dai A, Xie S, Zhou Y, Qin J, Sun JP, Scharf DH, Hou T, Zhou T, Wang MW, Zhang Y
Cell Res (2021) 31 pp. 1163-1175 [ Pubmed: 34433901 DOI: doi:10.1038/s41422-021-00552-3 ]
- Melanocrotin-4 receptor (Complex from Homo sapiens)
- Complex of thiq bound mc4r-gs protein (180 kDa, Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Dominant negative guanine nucleotide-binding protein g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from synthetic construct)
- Nanobody35 (Complex)
- (3r)-n-[(2r)-3-(4-chlorophenyl)-1-[4-cyclohexyl-4-(1,2,4-triazol-1-ylmethyl)piperidin-1-yl]-1-oxidanylidene-propan-2-yl]-1,2,3,4-tetrahydroisoquinoline-3-carboxamide (589 Da, Ligand)
- Melanocortin receptor 4 (56 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of the P395-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfm
Q-score: 0.517
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ DOI: doi:10.1038/s41586-020-2569-1 Pubmed: 32698187 ]
- 2-(ethylamino)-6-[3-(4-propan-2-ylphenyl)propanoyl]-7,8-dihydro-5h-pyrido[4,3-d]pyrimidine-4-carboxamide (395 Da, Ligand)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- P395-bound gpbar-gs complex (Complex)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- P395 (Complex from synthetic construct)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Gpbar (Complex from Homo sapiens)
- Gs (Complex from Homo sapiens)
Cryo-EM structure of the INT-777-bound GPBAR-Gs complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7cfn
Q-score: 0.53
Yang F, Mao C, Guo L, Lin J, Ming Q, Xiao P, Wu X, Shen Q, Guo S, Shen DD, Lu R, Zhang L, Huang S, Ping Y, Zhang C, Ma C, Zhang K, Liang X, Shen Y, Nan F, Yi F, Luca VC, Zhou J, Jiang C, Sun JP, Xie X, Yu X, Zhang Y
Nature (2020) 587 pp. 499-504 [ DOI: doi:10.1038/s41586-020-2569-1 Pubmed: 32698187 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein subunits (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Int-777-bound gpbar-gs complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- G-protein coupled bile acid receptor 1 (Complex)
- G-protein coupled bile acid receptor 1 (35 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from Homo sapiens)
- (2s,4r)-4-[(3r,5s,6r,7r,8r,9s,10s,12s,13r,14s,17r)-6-ethyl-10,13-dimethyl-3,7,12-tris(oxidanyl)-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methyl-pentanoic acid (450 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)