Cryo-EM structure of Human SHMT1
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 8r7h
Q-score: 0.439
Spizzichino S, Di Fonzo F, Marabelli C, Tramonti A, Chaves-Sanjuan A, Parroni A, Boumis G, Liberati FR, Paone A, Montemiglio LC, Ardini M, Jakobi AJ, Bharadwaj A, Swuec P, Tartaglia GG, Paiardini A, Contestabile R, Mai A, Rotili D, Fiorentino F, Macone A, Giorgi A, Tria G, Rinaldo S, Bolognesi M, Giardina G, Cutruzzola F
Mol Cell (2024) 84 pp. 2682- [ DOI: doi:10.1016/j.molcel.2024.06.016 Pubmed: 38996576 ]
- Serine hydroxymethyltransferase, cytosolic (53 kDa, Protein from Homo sapiens)
- Human shmt1 (210 kDa, Complex from Homo sapiens)
- Serine hydroxymethyltransferase, cytosolic (53 kDa, Protein from Homo sapiens)
Cryo-EM structure of the Human SHMT1-RNA complex
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 8a11
Q-score: 0.395
Spizzichino S, Di Fonzo F, Marabelli C, Tramonti A, Chaves-Sanjuan A, Parroni A, Boumis G, Liberati FR, Paone A, Montemiglio LC, Ardini M, Jakobi AJ, Bharadwaj A, Swuec P, Tartaglia GG, Paiardini A, Contestabile R, Mai A, Rotili D, Fiorentino F, Macone A, Giorgi A, Tria G, Rinaldo S, Bolognesi M, Giardina G, Cutruzzola F
Mol Cell (2024) [ DOI: doi:10.1016/j.molcel.2024.06.016 Pubmed: 38996576 ]
- Pyridoxal-5'-phosphate (247 Da, Ligand)
- Serine hydroxymethyltransferase, cytosolic (53 kDa, Protein from Homo sapiens)
- Human shmt1 in complex with rna (shmt2 5' utr 1-50) (53 MDa, Complex from Homo sapiens)
cAMP-free rabbit HCN4 stabilized in LMNG-CHS detergent mixture
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7np3
Q-score: 0.419
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929-2943.e6 [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]
cAMP-bound rabbit HCN4 stabilized in LMNG-CHS detergent mixture
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7np4
Q-score: 0.451
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929-2943.e6 [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]
Rabbit HCN4 stabilised in amphipol A8-35
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7nmn
Q-score: 0.459
Saponaro A, Bauer D, Giese MH, Swuec P, Porro A, Gasparri F, Sharifzadeh AS, Chaves-Sanjuan A, Alberio L, Parisi G, Cerutti G, Clarke OB, Hamacher K, Colecraft HM, Mancia F, Hendrickson WA, Siegelbaum SA, DiFrancesco D, Bolognesi M, Thiel G, Santoro B, Moroni A
Mol Cell (2021) 81 pp. 2929- [ DOI: doi:10.1016/j.molcel.2021.05.033 Pubmed: 34166608 ]
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rj9
Q-score: 0.542
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Ntpam (Complex from Streptococcus virus 2972)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 monomeric assembly. (Complex)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Acriia6 (Complex from Streptococcus phage D1811)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
- Sgrna (Complex from Streptococcus thermophilus)
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rja
Q-score: 0.508
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 dimeric assembly. (Complex)
- Tdna20 (Complex from Streptococcus virus 2972)
- Acriia6 (Complex from Streptococcus phage D1811)
- Rna (78-mer) (Complex from Streptococcus thermophilus)
- Rna (78-mer) (37 kDa, RNA from Streptococcus thermophilus)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
Cryo-EM structure of St1Cas9-sgRNA-tDNA59-ntPAM complex.
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6rjd
Q-score: 0.5
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Ntpam (Complex from Streptococcus phage 2972)
- Tdna59 (18 kDa, DNA from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna59-ntpam complex. (Complex)
- Sgrna (78-mer) (Complex from Streptococcus thermophilus)
- Ntpam (7 kDa, DNA from Streptococcus Phage 2972)
- Streptococcus thermophilus 1 cas9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Sgrna (78-mer) (37 kDa, RNA from Streptococcus thermophilus DGCC 7710)
- Streptococcus thermophilus 1 cas9 (Complex from Streptococcus thermophilus DGCC 7710)
- Tdna59 (Complex from Streptococcus phage D1811)
Cryo-EM structure of St1Cas9-sgRNA-AcrIIA6-tDNA59-ntPAM complex.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rjg
Q-score: 0.522
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Ntpam (Complex from Streptococcus virus 2972)
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Ntpam (7 kDa, DNA from Streptococcus virus 2972)
- Cas 9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Cas 9 (Complex from Streptococcus thermophilus DGCC 7710)
- Tdna59 (18 kDa, DNA from Streptococcus virus 2972)
- Tdna59 (Complex from Streptococcus virus 2972)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Acriia6 (Complex from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-acriia6-tdna59-ntpam complex. (Complex)
- Sgrna (Complex from Streptococcus thermophilus)