Stabilized beta-arrestin 1-V2T subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6ni2
Q-score: 0.348
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- Fab30 light chain (23 kDa, Protein from synthetic construct)
- Beta-arrestin-1 (Complex from Bos taurus)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Beta-arrestin-1 (44 kDa, Protein from Bos taurus)
- Fab30 heavy chain (25 kDa, Protein from synthetic construct)
- Stabilized beta-arrestin 1-v2t subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- Vasopressin 2 tail (Complex from Homo sapiens)
- Fab30 (Complex from synthetic construct)
- Nanobody 32 (Complex from Lama glama)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ni3
Q-score: 0.39
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- B2v2r-gs protein subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- B2v2r-gs protein subcomplex (Complex from Homo sapiens)
- Endolysin,beta-2 adrenergic receptor chimera (57 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- 8-[(1r)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2h-1,4-benzoxazin-3(4h)-one (370 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
Structure of a GPCR-G protein-beta-arrestin mega-complex
Nguyen AH, Thomsen ARB, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]