Cryo_EM structure of human FAN1 R507H mutant in complex with 5' flap DNA substrate and PCNA
Aretz J, Jeyasankar G, Salerno-Kochan A, Thomsen M, Thieulin-Pardo G, Haque T, Monteagudo E, Felsenfeld D, Finley M, Vogt TF, Boudet J, Prasad BC
bioRxiv (2024) [ DOI: doi:10.1101/2024.10.09.617442 ]
- Ternary complex of fan1 r507h-pcna-dna (Complex)
- Proliferating cell nuclear antigen (28 kDa, Protein from Homo sapiens)
- Dna (post-nick) (6 kDa, DNA from Homo sapiens)
- Dna (Complex from Homo sapiens)
- Dna (pre-nick) (8 kDa, DNA from Homo sapiens)
- Dna (continuous) (12 kDa, DNA from Homo sapiens)
- Fan1 r507h-pcna (Complex from Homo sapiens)
- Fanconi-associated nuclease 1 (114 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
(CAG)2 DNA-bound MutSbeta in open form with kinked MSH2 clamp
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 8rz7
Q-score: 0.292
Lee J-H, Thomsen M, Daub H, Steinbacher S, Sztyler A, Thieulin-Pardo G, Neudegger T, Plotnikov N, Iyer RR, Wilkinson H, Monteagudo E, Felsenfeld DP, Haque T, Finley M, Dominguez C, Vogt TF, Prasad BC
To Be Published
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna mismatch repair protein (Complex from Homo sapiens)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Dna_1 (20 kDa, DNA from Homo sapiens)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)
- Mutsbeta-dna-adp complex (Complex)
- Double stranded dna (Complex from Homo sapiens)
- Dna_2 (18 kDa, DNA from Homo sapiens)
MutSbeta-ATPgS with straight MSH2 clamp
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 8rz8
Q-score: 0.349
Lee J-H, Thomsen M, Daub H, Steinbacher S, Sztyler A, Thieulin-Pardo G, Neudegger T, Plotnikov N, Iyer RR, Wilkinson H, Monteagudo E, Felsenfeld DP, Haque T, Finley M, Dominguez C, Vogt TF, Prasad BC
To Be Published
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Mutsbeta-atpgs complex (Complex from Homo sapiens)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)
MutSbeta-ATPgS with kinked MSH2 clamp
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8rz9
Q-score: 0.332
Lee J-H, Thomsen M, Daub H, Steinbacher S, Sztyler A, Thieulin-Pardo G, Neudegger T, Plotnikov N, Iyer RR, Wilkinson H, Monteagudo E, Felsenfeld DP, Haque T, Finley M, Dominguez C, Vogt TF, Prasad BC
To Be Published
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Mutsbeta-atpgs complex (Complex from Homo sapiens)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)
cryo-EM Structure of Craf:14-3-3:Mek1
Resolution: 4.25 Å
EM Method: Single-particle
Fitted PDBs: 8chf
Q-score: 0.18
Dedden D, Nitsche J, Schneider EV, Thomsen M, Schwarz D, Leuthner B, Gradler U
J Mol Biol (2024) 436 pp. 168483-168483 [ DOI: doi:10.1016/j.jmb.2024.168483 Pubmed: 38331211 ]
- Raf proto-oncogene serine/threonine-protein kinase (73 kDa, Protein from Homo sapiens)
- Complex of dimer phosphorylated c-raf kinase domain with 14-3-3 dimer and 2 subunits of mek1 containing ligand gdc-0623 (Complex from Homo sapiens)
- 14-3-3 protein zeta isoform x1 (28 kDa, Protein from Spodoptera frugiperda)
- Dual specificity mitogen-activated protein kinase kinase 1 (43 kDa, Protein from Homo sapiens)
- 2-{4-[(1e)-1-(hydroxyimino)-2,3-dihydro-1h-inden-5-yl]-3-(pyridin-4-yl)-1h-pyrazol-1-yl}ethanol (334 Da, Ligand)
MutSbeta bound to (CAG)2 DNA (canonical form)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8olx
Q-score: 0.397
Lee JH, Thomsen M, Daub H, Thieulin-Pardo G, Steinbacher SC, Sztyler A, Dahiya V, Neudegger T, Dominguez C, Iyer RR, Wilkinson HA, Monteagudo E, Plotnikov NV, Felsenfeld DP, Haque TS, Finley M, Boudet J, Vogt TF, Prasad BC
bioRxiv (2024) [ DOI: doi:10.1101/2023.05.08.539930 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Dna (27-mer) (8 kDa, DNA from synthetic construct)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)
- Mutsbeta bound to (cag)2 dna (canonical form) (Complex from Homo sapiens)
- Dna (21-mer) (6 kDa, DNA from synthetic construct)
DNA-free open form of MutSbeta
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 8om5
Q-score: 0.3
Lee J-H, Thomsen M, Daub H, Steinbacher S, Sztyler A, Thieulin-Pardo G, Neudegger T, Plotnikov N, Iyer RR, Wilkinson H, Monteagudo E, Felsenfeld DP, Haque T, Finley M, Dominguez C, Vogt TF, Prasad BC
To Be Published
- Dna-free open form of mutsbeta (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)
MutSbeta bound to (CAG)2 DNA (open form)
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8om9
Q-score: 0.249
Lee J-H, Thomsen M, Daub H, Steinbacher S, Sztyler A, Thieulin-Pardo G, Neudegger T, Plotnikov N, Iyer RR, Wilkinson H, Monteagudo E, Felsenfeld DP, Haque T, Finley M, Dominguez C, Vogt TF, Prasad BC
To Be Published
- Magnesium ion (24 Da, Ligand)
- Dna2 (6 kDa, DNA from Homo sapiens)
- Mutsbeta bound to (cag)2 dna (open form) (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)
- Dna1 (8 kDa, DNA from Homo sapiens)
MutSbeta bound to 61bp homoduplex DNA
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 8oma
Q-score: 0.177
Lee J-H, Thomsen M, Daub H, Steinbacher S, Sztyler A, Thieulin-Pardo G, Neudegger T, Plotnikov N, Iyer RR, Wilkinson H, Monteagudo E, Felsenfeld DP, Haque T, Finley M, Dominguez C, Vogt TF, Prasad BC
To Be Published
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Mutsbeta bound to 61bp homoduplex dna (Complex from Homo sapiens)
- Dna1 (5 kDa, DNA from Homo sapiens)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Dna2 (5 kDa, DNA from Homo sapiens)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)
DNA-unbound MutSbeta-ATP complex (bent clamp form)
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8omo
Q-score: 0.318
Lee J-H, Thomsen M, Daub H, Steinbacher S, Sztyler A, Thieulin-Pardo G, Neudegger T, Plotnikov N, Iyer RR, Wilkinson H, Monteagudo E, Felsenfeld DP, Haque T, Finley M, Dominguez C, Vogt TF, Prasad BC
To Be Published
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna-unbound mutsbeta-atp complex (bent clamp form) (Complex from Homo sapiens)
- Dna mismatch repair protein msh2 (104 kDa, Protein from Homo sapiens)
- Dna mismatch repair protein msh3 (127 kDa, Protein from Homo sapiens)