Cryo-EM map of human symplekin CTD
Sun Y, Zhang Y, Aik WS, Yang XC, Marzluff WF, Walz T, Dominski Z, Tong L
Science (2020) 367 pp. 700-703 [ DOI: doi:10.1126/science.aaz7758 Pubmed: 32029631 ]
Cryo-EM map of the core of human histone pre-mRNA cleavage complex (CPSF73-CPSF100-Symplekin)
Sun Y, Zhang Y, Aik WS, Yang XC, Marzluff WF, Walz T, Dominski Z, Tong L
Science (2020) 367 pp. 700-703 [ DOI: doi:10.1126/science.aaz7758 Pubmed: 32029631 ]
Cryo-EM map of the overall structure of human histone pre-mRNA 3'-end processing machinery
Sun Y, Zhang Y, Aik WS, Yang XC, Marzluff WF, Walz T, Dominski Z, Tong L
Science (2020) 367 pp. 700-703 [ DOI: doi:10.1126/science.aaz7758 Pubmed: 32029631 ]
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6v4x
Q-score: 0.516
Sun Y, Zhang Y, Aik WS, Yang XC, Marzluff WF, Walz T, Dominski Z, Tong L
Science (2020) 367 pp. 700-703 [ DOI: doi:10.1126/science.aaz7758 Pubmed: 32029631 ]
- Zinc ion (65 Da, Ligand)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Symplekin (120 kDa, Protein from Homo sapiens)
- Modified h2a pre-mrna (16 kDa, RNA from Mus musculus)
- U7 snrna (19 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (10 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (16 kDa, Protein from Homo sapiens)
- U7 snrna-associated sm-like protein lsm10 (14 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 3 (77 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 2 (88 kDa, Protein from Homo sapiens)
- U7 snrna-associated sm-like protein lsm11 (28 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- The core of metazoan replication-dependent histone pre-mrna 3'-end processing machinery (Complex from Homo sapiens)
Cryo-EM structure of human CPSF-160-WDR33-CPSF-30-PAS RNA complex at 3.4 A resolution
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6dnh
Q-score: 0.512
Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L
PNAS (2018) 115 pp. E1419-E1428 [ Pubmed: 29208711 DOI: doi:10.1073/pnas.1718723115 ]
- Zinc ion (65 Da, Ligand)
- Cleavage and polyadenylation specificity factor subunit 4 (27 kDa, Protein from Homo sapiens)
- Quaternary complex of human cpsf-160-wdr33-cpsf-30 with aauaaa polyadenylation signal (255 kDa, Complex)
- Cpsf-30 (27 kDa, Complex from Homo sapiens)
- Rna (5'-r(p*ap*ap*up*ap*ap*ap*c)-3') (5 kDa, RNA from Simian virus 40)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Cpsf-160-wdr33 (225 kDa, Complex from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)
- Aauaaa polyadenylation signal (5 kDa, Complex from Simian virus 40)
Cryo-EM structure of human CPSF-160-WDR33 complex at 3.36 A resolution
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 6bly
Q-score: 0.513
Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L
PNAS (2018) 115 pp. E1419-E1428 [ Pubmed: 29208711 DOI: doi:10.1073/pnas.1718723115 ]
- Binary complex of human cpsf-160-wdr33 (225 kDa, Complex from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)
Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6bm0
Q-score: 0.431
Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L
PNAS (2018) 115 pp. E1419-E1428 [ Pubmed: 29208711 DOI: doi:10.1073/pnas.1718723115 ]
- Binary complex of human cpsf-160-wdr33 (225 kDa, Complex from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)