Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8pm2
Q-score: 0.496
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.07.547762 Pubmed: 37461561 ]
- Trace amine-associated receptor 7f (41 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- A complex of mouse trace-amine associated receptor 7f solubilized in lmng/chs bound to n,n-dimethylcyclohexylamine and coupled to: engineered guanine nucleotide-binding protein g(s) subunit alpha isoform short + guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 + guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 + nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody 35 (16 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
Class D GPCR Ste2 dimer coupled to two G proteins
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ad3
Q-score: 0.455
Velazhahan V, Ma N, Pandy-Szekeres G, Kooistra AJ, Lee Y, Gloriam DE, Vaidehi N, Tate CG
Nature (2021) 589 pp. 148-153 [ DOI: doi:10.1038/s41586-020-2994-1 Pubmed: 33268889 ]
- Guanine nucleotide-binding protein alpha-1 subunit,guanine nucleotide-binding protein alpha-1 subunit (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cholesterol hemisuccinate (486 Da, Ligand)
- Alpha-factor mating pheromone (1 kDa, Protein from Saccharomyces cerevisiae)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein alpha-1 subunit,guanine nucleotide-binding protein alpha-1 subunit (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ste2 dimer coupled to two g proteins (Complex from Saccharomyces cerevisiae)
- Ste4 isoform 1 (46 kDa, Protein from Saccharomyces cerevisiae)
- Ste2 dimer coupled to two g proteins (266 kDa, Complex)
- Guanine nucleotide-binding protein subunit gamma (12 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Alpha-factor mating pheromone (Complex from Saccharomyces cerevisiae)
Structure of the class D GPCR Ste2 dimer coupled to two G proteins
Velazhahan V, Ma N, Pándy-Szekeres G, Kooistra AJ, Lee Y, Gloriam DE, Vaidehi N, Tate CG
Nature (2020) 589 pp. 148-153 [ Pubmed: 33268889 DOI: 10.1038/s41586-020-2994-1 ]
Image sets: